We narrowed to 526 results for: sequence

... and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional and lab...to the associated data (spreadsheets, images, sequencing data, etc.) used and/or referenced in the experiment...

...examined approximately 1,900 sgRNAs and identified sequence features and adjustments that improved sgRNA activity...Tanenbaum et al., 2014). The sgRNA or tracrRNA sequences can also be modified to include RNA domains that...

...circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that...of proteins that are highly similar in terms of sequence and thus very difficult to distinguish using standard...

... still able to bind to the upstream activation sequence (UAS) of its target gene, in this case a reporter...downstream of a promoter containing the target sequence of the DBD. The DBD-POI fusion (commonly referred...

...However, iChIP requires insertion of recognition sequences of an exogenous DNA-binding protein such as LexA...to suppress PCR amplification of specific DNA sequences. Thus, we've made some contributions in technology...

...is quality controlled through next generation sequencing and spectrophotometry to test purity (260/280...

... 3-6 letter representation of the gene (or DNA sequence). pBACKBONE-hGene Tip: Often researchers will ...

...data and images from the hiPS cell lines gRNA sequences used in the creation of the hiPS cell lines The...

...than BE3, but APOBEC3G displayed less predictable sequence preferences.  The Liu lab also used natural and...develop five new base editors with distinct PAM sequences, expanding the number of available target sites...evolution efforts yielded an editor with more flexible sequence preferences, evoAPOBEC1-BE4max, which is significantly...editing, has allowed scientists to replace entire sequences of varying lengths. Base editing publication ...

...testing, and sterility testing, plus viral genomic sequencing to confirm the identity of the packaged DNA and...

...for your research.     Improved nucleic acid sequence detection Feng Zhang’s lab recently developed ...

...science and more! All Posts from October 2016: Sequencing Options for CRISPR Genotyping Grad School Advice...

...Addgenies working to ship plasmids, analyze QC sequence, answer scientists' questions, manage our website...

...the University of California, Santa Cruz, offers sequence data on five strains of Ebola representing the...

...and archaeal immune systems has led to extreme sequence divergence that requires a multi-pronged analysis...classification system relies on a combination of sequence similarity, phylogenetic analysis, neighborhood...that are in the same class and type don’t share sequence similarity but are grouped due to mechanisms of...

... replication problem. Iterons are repeated sequences within the origin of replication and are required...how compatible their plasmids will be based on sequence data. However, if these do not provide clarity...

...discussions in my lab about whether or not to keep DNA sequencing singletons (reads that only appear once) in our...analysis pipelines for DNA that group similar sequences together in bins, we thought that binning Singletons...

...the EBNA-1 sequence that encodes for the DNA binding protein EBNA-1 [7]. These two sequences are necessary...

...unable to methylate cytosine within a particular sequence. Browse Addgene's curated list of Bacterial Expression...-deficient; best for plasmids with repetative sequences.  F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) Δ...

...services: We continue to offer high-quality DNA sequencing, viral vector preparations, and antibody production...establish.These funding decisions will have far-reaching consequences throughout the scientific community for years...