... 6 Tips for Analyzing and Troubleshooting DNA Sequencing Results ICYMI: Addgene’s Plasmids 101 eBook Protocols...

...Addgenies working to ship plasmids, analyze QC sequence, answer scientists' questions, manage our website...

...even be able to alter the type of nucleic acid sequences that Cas9 can target." As further proof of just...

...generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are...

...constructing TALE repeat arrays of any desired sequence, referred to as the REAL (Restriction Enzyme And...

...accessibility in research and therefore makes the sequences of these affinity reagents available to scientists...

...repeat arrays with identical DNA and amino acid sequences (i.e. only the details of the assembly are different...

...the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This well-known motif serves... of the 11 IPI antibodies contain RGD-mimetic sequences that imitate this motif and compete with both ...

...phosphoserine incorporation Extensive genomic sequencing of prokaryotic lineages revealed a curious anomaly...translation in response to the presence of TAG sequences in ORFs. In this bacterial strain, TAG is an ‘...

...for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher’s collaborator...

...for plasmid mapping, DNA/protein databases, DNA sequence manipulation, and primer design. Best wishes ...

...promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express...

...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system...

...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...

... 5’ end to base pair with a target genomic DNA sequence and a nuclease (e.g.—the Cas9 endonuclease) that...

...DNA strand (in cis) in the same direction, the sequence between the sites is deleted as a circular piece... allows for recombination of LoxP flanked DNA sequences. Use the search box below to find the perfect ... 25 nucleotide extracellular vesicle targeting sequence and CFP UBC Mammalian van Rheenen 65795 pBBI iCre...Cre activates gene of interest by removing Stop sequences; Puro selection. See plasmid 35688 for Hygro selection...Cre activates gene of interest by removing Stop sequences. Can be used for injection into fertilized zygotes...activates gene of interest by removing neoR and stop sequence; used for making transgenic mice. Plasmid 15037...

...Each isoform is uniquely barcoded with a 24 bp sequence, to simplify identification during screening. ...

...using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive genomic locus...

...control over gene expression that go beyond genomic sequence. Dysregulation of the epigenome (the sum of epigenetic...epigenetic modifier could be targeted to specific DNA sequences. Non-editing CRISPR applications direct catalytically...

...subtilis, contains the MISTIC (membrane-integrating sequence for translation of integral-membrane protein constructs...