We narrowed to 538 results for: sequence
-
TypeCollection...accessibility in research and therefore makes the sequences of these affinity reagents available to scientists...
-
TALEN Expression Vectors
TypeCollection...repeat arrays with identical DNA and amino acid sequences (i.e. only the details of the assembly are different... -
Typing CRISPR Systems
TypeBlog Post...and archaeal immune systems has led to extreme sequence divergence that requires a multi-pronged analysis...classification system relies on a combination of sequence similarity, phylogenetic analysis, neighborhood...that are in the same class and type don’t share sequence similarity but are grouped due to mechanisms of... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...the EBNA-1 sequence that encodes for the DNA binding protein EBNA-1 [7]. These two sequences are necessary... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post... replication problem. Iterons are repeated sequences within the origin of replication and are required...how compatible their plasmids will be based on sequence data. However, if these do not provide clarity... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...discussions in my lab about whether or not to keep DNA sequencing singletons (reads that only appear once) in our...analysis pipelines for DNA that group similar sequences together in bins, we thought that binning Singletons... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...unable to methylate cytosine within a particular sequence. Browse Addgene's curated list of Bacterial Expression...-deficient; best for plasmids with repetative sequences. F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) Δ... -
Addgene Stands with the Scientific Community
TypeBlog Post...services: We continue to offer high-quality DNA sequencing, viral vector preparations, and antibody production...establish.These funding decisions will have far-reaching consequences throughout the scientific community for years... -
More Data for You: Find Articles Citing Addgene Plasmids
TypeBlog Post... 6 Tips for Analyzing and Troubleshooting DNA Sequencing Results ICYMI: Addgene’s Plasmids 101 eBook Protocols... -
500,000 Plasmids Shipped and Counting!
TypeBlog Post...Addgenies working to ship plasmids, analyze QC sequence, answer scientists' questions, manage our website... -
Keeping Up With CRISPR/Cas9
TypeBlog Post...even be able to alter the type of nucleic acid sequences that Cas9 can target." As further proof of just... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection...promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express... -
CRISPR Plasmids - RNA Editing
TypeCollection...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system... -
CRISPR Plasmids - Activate Gene Expression
TypeCollection...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection...Each isoform is uniquely barcoded with a 24 bp sequence, to simplify identification during screening. ... -
CRISPR Plasmids for Genomic Visualization
TypeCollection...using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive genomic locus... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...phosphoserine incorporation Extensive genomic sequencing of prokaryotic lineages revealed a curious anomaly...translation in response to the presence of TAG sequences in ORFs. In this bacterial strain, TAG is an ‘... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This well-known motif serves... of the 11 IPI antibodies contain RGD-mimetic sequences that imitate this motif and compete with both ... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog Post...for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher’s collaborator... -
Addgene's Top Blog Posts from 2020
TypeBlog Post...for plasmid mapping, DNA/protein databases, DNA sequence manipulation, and primer design. Best wishes ...