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We narrowed to 954 results for: app

Showing: 31 - 60 of 954 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach has been broadly...gene or projection). Intersectional expression approaches combine multiplexed recombinases, including Cre...broadly applied using multiple recombinase-expression strategies to define cellular sub-populations of interest... Oberhuber M, Conzelmann KK, Staiger JF. 2019. Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing... Dombeck DA, Deisseroth K, Awatramani R. 2018. Mapping projections of molecularly defined dopamine neuron...neuron subtypes using intersectional genetic approaches. Nat Neurosci. 21(9):1260-1271. PubMed (Link opens...

  3. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...generate due to expense and time. An alternative approach is to use synthetically produced recombinant antibodies...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...single domain antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. ...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...tTA-Advanced Takeshi Imai Other Tet Applications Explore some other popular applications of tetracycline systems ... of interest from one plasmid. A single vector approach may not have as much flexibility and control as...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated...154939 dCas13d-dsRBD-APEX2 RNA-protein interaction mapping via Cas13-based APEX targeting Alice Ting 104102...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat Chem...sensor's behavior in the target system. Use care when applying a biosensor in a novel context or modifying an...indicators based on mRuby (jRCaMP1a, jRCaMP1b) and mApple (jRGECO1a) (Constitutive or Cre-dependent) Sensitive... Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Sci Rep....NADP+ ratio or NAD+ Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine...Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry...Positron (Constitutive or Cre-dependent) A general approach to engineer positive-going eFRET voltage indicators...

  6. Antibodies

    Type
    Collection
    ...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...utility for the study of non-coding elements. This approach can be used for efficient loss-of-function studies...example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom (Crick) strand while sgRNA-B... the presence or absence of deletion. For this approach, the internal primers described in step 2.1 may...Phosphorylated oligos may be ordered instead. For this approach, the use of T4 PNK is omitted. Anneal in a thermocycler... to performing this step to reliably obtain approximately 30 cells per 96-well plate. Include 100 μl per...

  8. Bacterial Expression Systems

    Type
    Collection
    ...sequence? Do these two proteins interact? What happens when I overexpress this enzyme at a particular ...cellular activity in a way that’s interesting from an applied perspective, sometimes it’s best to delete genes...bacteria of interest are transformed with the appropriate construct, grown at the permissive temperature...localization of your gene of interest once its appropriate cDNA has been fused to the MS2 binding sites ...

  9. Rett Syndrome

    Type
    Collection
    ...females at an incidence of approximately 1:10,000. It is characterized by apparently normal development through...In females, random X inactivation results in approximately half of cells expressing wild-type MECP2 and...Tools CRISPR technology is a promising therapeutic approach for precise editing of mutations in the MECP2 ...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...advantages and disadvantages depending on the application. Firefly luciferase is considerably brighter ...luciferase-based plasmid systems for many different applications. Browse the Collection Highlights section to...respectively, for increased usefulness in in vivo applications. LuxSit-i : An artificial luciferase desined...desined de novo using deep-learning approaches. Split-Luciferase and Protein-Protein Interaction Assays Cluc-FKBP...

  11. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...insertions — meaning they may not be suitable for applications where orientation of the insert is important... TnsC, and TniQ). Unlike type I, type V-K CASTs appear to exhibit almost unidirectional insertions, but...

  12. Institute for Protein Innovation

    Type
    Collection
    ...Antibody Description Isotype Reactivity Recommended Applications Integrin Antibodies Integrins are a family of...Antibody Description Isotype Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI has developed...Antibody Description Isotype Reactivity Recommended Applications Browse Antibody Plasmids The following plasmids...

  13. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...subject to Addgene’s Terms of Purchase and the applicable material transfer agreements (MTAs) and limited...generally ready for shipment within 8 weeks after the approval of the MTA by your institution. Our final step...contact you directly regarding these charges, if applicable....

  14. Distribution to Industry

    Type
    Collection
    ...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don’t See What You’re Looking For? Please...email to [email protected] . After we review and approve the MTA documents, your order will be processed...

  15. AAVED

    Type
    Collection
    ...useful to help improve this technology or its application? What are some current obstacles that researchers...antibodies and recommended dilutions, etc.), novel applications, or any other useful information. Experimental...take care of that. Note that you will have final approval before anything is released publicly, but please...

  16. Microbiology Resources

    Type
    Collection
    ...our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...Densmore Lab EcoFlex MoClo : Modular cloning for applications like recombinant protein purification and cell-free...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

  17. TALEN Guide

    Type
    Collection
    ...your dissertation. Well, that will probably never happen. Yet with transcription activator-like (TAL) effectors... they still have potential targetable sites approximately every 35bp. Researchers are still determining...arrays from 12-31 repeats. Perhaps one of the most appealing features of the TAL effector arrays is the ease...

  18. Brain Initiative Collection

    Type
    Collection
    ... the human brain through the development and application of innovative tools enabling large-scale real-time.... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting 163909-AAV9 pAAV_hSynapsin_psychLight2...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Additional Resources To learn more, please...

  19. COVID-19 Resources

    Type
    Collection
    ... antibody escape mutants and for comprehensive mapping. Some of the following plasmids are available to... Spike protein RBD mutations for comprehensive mapping (Jesse Bloom). SARS-CoV-2 Spike Receptor Binding... Spike protein RBD mutations for comprehensive mapping (Jesse Bloom, Tyler Starr). External Resources ...

  20. Zhang Lab CRISPR Page

    Type
    Collection
    ... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

  21. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...neurodegeneration research: Anti-MAPT [AT8] and Anti-APP [6E10-A5]. Both are recombinant mouse monoclonal ...Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant antibody in human postmortem... antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene...in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots. But Jae-Yean...efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024). Their optimized strategy uses... wild type (WT), two weeks after herbicide was applied to both plants. Adapted from Vu et al., 2024 bioRxiv...CC-BY-NC-ND 4.0 International license. With this approach, the team was able to drive prime editing in both...

  22. Validated gRNA Sequences

    Type
    Collection
    ...upstream of a 5' NGG 3' PAM sequence). Which CRISPR application is this gRNA sequence compatible with? CRISPR...Resources page have been used to indicate the Cas9 application the gRNA was designed to accomplish. Validated...Gene Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens...

  23. Tags and Other Markers

    Type
    Collection
    ...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

  24. Neuroscience

    Type
    Collection
    ...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

  25. Fluorescent Protein Guide

    Type
    Collection
    ...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins (FPs) and to find the...

  26. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...upstream of a 5' NGG 3' PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR...

  27. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled..., some CRISPR-Cas3 plasmids take an all-in-one approach and include Cas3 and Cascade on one single plasmid...

  28. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene targets... standard desalted oligonucleotides with the appropriate overhangs: The plasmids listed here are provided...

  29. Resolute Plasmid Collection

    Type
    Collection
    ...family of transporters in the human genome with approximately 450 identified members. Many SLCs have mutations... benefit of basic academic research as well as applied research in small biotech and pharmaceutical companies...

  30. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...single round of viral entry and infection. This approach allows researchers to safely study mechanisms ...for SARS-CoV-2 and other coronaviruses using appropriate packaging plasmids. Use the search bar to find...
Showing: 31 - 60 of 954 results