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We narrowed to 210 results for: cycs

Showing: 31 - 60 of 210 results

  1. An Introduction to Adenovirus

    Type
    Blog Post
    ...exist. The life cycle of AdVs is dependent on modulation of the host cell’s cell cycle; inhibition of ...., 2003).        Figure 1: The adenovirus life cycle. Image reused from Kremer and Nemerow 2015 under...

  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...stimulate quiescence, the resting phase of the cell cycle; quiescent cells do not take up nucleic acid or ...lentiviral transduction. The effect of freeze-thaw cycles on lentiviral stability depends on a variety of...produced at pH 6 are resistant to multiple freeze thaw cycles. Consequently, it is best to avoid freeze-thawing...

  3. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...not recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG...T7RNAP-tetA General protein expression  BLR (DE3) Tetracycline RecA-deficient; best for plasmids with repetative...proteins Origami2 (DE3)** Streptomycin and Tetracycline Contains highly active thioredoxin reductase...

  4. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP." Journal of the American Chemical...et al., 2017) and levels of the second messenger cyclic di-AMP in Gram-positive bacteria (Kellenberger ...

  5. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... streptomycin Tn10   Confers resistance to tetracycline Additionally, Table 2 provides a quick reference...galE15 galK16 rpsL(StrR) endA1 λ- XL1 Blue Tetracycline Blue/white screening and routine cloning, nalidixic...lacIq Δ(lacZ)M15] hsdR17(rK- mK+)  XL10 Gold Tetracycline and Chloramphenicol High competency cloning ...

  6. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... against the Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene...expression by administering an inducing agent like doxycycline (Favre et al., 2002). They are useful when you...

  7. What's New in CRISPR - March 2020

    Type
    Blog Post
    ... model organism for studying the bacterial cell cycle, asymmetric cell division, and cellular differentiation...repressed ctrA, a gene encoding an essential cell cycle regulator. The lab expressed both dCas9 and the ...

  8. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...dynamics, and how they change in response to the cell cycle or other stimuli.   To create a colorful Cas9 toolbox...technique will be applicable to studies of cell cycle progression, epigenetics and cellular reactions ...

  9. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...and Paul Greengard. "Dopamine-sensitive adenyl cyclase: possible role in synaptic transmission." Science... Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its similarity...

  10. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...techniques: unwanted mutations due to excessive PCR cycles or low fidelity Taq DNA polymerase, the construction...high fidelity DNA polymerases like phusion. An 18-cycle PCR leads to the most colonies after transformation...

  11. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ... of XFPs in the original method, we used the Tetracycline trans-activator system to boost the expression...expression levels, and we named the system Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al.,...

  14. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...reporter mRNAs. Using translation inhibitors cycloheximide and puromycin, Halstead et al. verified that...effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing that ~80% of transcripts with...

  16. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...you’re working with them, but avoid freeze-thaw cycles, as they’ll degrade your antibody.  At some point...been left out, gone through a freeze-thaw-freeze cycle before, or otherwise been subject to mishandling...

  17. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...for infection of quiescent cells or infrequently cycling cells but is fine for experiments with immortalized...integration to express its cargo, in fact its viral life cycle does not include genomic integration. While the ...

  20. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...some viral preps are very sensitive to freeze thaw cycles; the titer measured with a freshly collected lentiviral...aliquot that has been subjected to a freeze/thaw cycle for titering as this will better represent future...

  22. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ... to increase yield. The collection process is a cycle of collecting the media and adding fresh media every...Baculovirus replicates inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the...

  23. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ... target cells and can be altered by freeze/thaw cycles (Lock et al., 2010). It is recommended that you...are quite stable and will withstand freeze/thaw cycles and dehydration (Howard & Harvey, 2017). You can...

  25. MXS Chaining

    Type
    Blog Post
    ..., one can generate constructs to visualize cell cycle dynamics, titrate inducible transgene expression...later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used ...

  26. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...few things you can try: Increase the number of cycles. After additional rounds of amplification, your...positive and negative droplets. No more than 50 cycles is recommended. Decrease your ramp rate. A rate...

  27. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...generally aliquoted to reduce the number of freeze-thaw cycles needed when using the virus. A small aliquot is...lentivirus. Since viruses are sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests...

  28. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...is tightly coupled to the bacterial host's cell cycle, maintaining a stable concentration of plasmid. ...copy number of pSC101 through a negative feedback cycle. As with DnaA, high concentrations of RepA negatively...

  29. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...splice acceptor. TRE General expression mRNA Tetracycline response element promoter Inducible with Tetracyline...promoter with low basal activity and several tetracycline operators. Transcription can be turned on or...

  30. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...observed in humans. Thus, understanding their life cycle and processes with their regenerative abilities ...observing and manipulating the entire developmental cycle. A majority of studies involving axolotls focus ...
Showing: 31 - 60 of 210 results