Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 253 results for: Mac

Showing: 41 - 80 of 253 results

  1. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...The most commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as ...the system. Since then, multiple biotech and pharmaceutical companies (along with some research laboratories...the expression of recombinant proteins by the pharmaceutical and biotech industries as a protein expression...specifically glycosylation. The native glycosylation machinery of K. phaffii lacks certain terminal mannose glycopeptides...

  2. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...therapeutic proteins. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft...further differentiated into dentritic cells or macrophages through cytokine induction (Groell et.al, 2018...Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis: From Manufacturing to Preclinical...

  3. dTAG - You're it!

    Type
    Blog Post
    ...Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close proximity with the target protein, ...degraders provide distinct advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest...to co-opt the endogenous ubiquitin proteasome machinery to degrade any protein. We are particularly excited...FKBP12F36V-fusion and the ubiquitin proteasome machinery through binding with cereblon, leading to rapid...

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ...repair-mediated Kras G12D mutations, leading to macroscopic tumors of adenocarcinoma pathology. These plasmids...

  7. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Kätzel D, Nissen W, Pekcec A (2019) Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide, clozapine...et al., 2019). Using PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules, were developed...be specifically and potently activated by pharmacologically selective effector molecules (PSEMs). Higher...

  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ... efficiency and building prediction tools with machine learning (PRIDICT, PRIDICT2.0).   How to apply...chromatin-based prediction tool (ePRIDICT). In summary, machine learning-based prime editing prediction models ...computational biology. His expertise includes applying machine learning techniques to make sense of biology, with...B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction of prime editing efficiency...

  9. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... will be recognized by its host’s replication machinery. A shuttle vector designed for unrelated bacteria...requires compatible cell lines that express viral machinery. Alternatively, some slow-growing cells, particularly...nutrient. The vector provides the missing synthesis machinery, and the cells are switched to minimal media, ...transfection into producer cells, which have the machinery to manufacture infectious viral particles. Those...

  10. The Challenges of Cell Culture

    Type
    Blog Post
    ...oncology, virology, immunology, microbiology, and pharmacology (many of which are represented in Addgene's ...cell culture over the last few decades some pharmacologists have tended to avoid cell culture in favour...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...

  11. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...., & MacRae, I. J. (2009). The RNA-induced Silencing Complex: a versatile gene-silencing machine. Journal... plasmids, shRNAs hijack the endogenous miRNA machinery for processing (Figure 1). The shRNA sequence ...the bound miRISC can simply block translation machinery. The composition of the RISC varies, but at the...

  12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...typical PCR machines, the authors use affordable, field-ready, Bluetooth-enabled PCR machines that can even...approximately 1-2 hours Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer...mentioned, likely ~1-2 hours Equipment needed: PCR machines/Heat blocks, Lateral flow strips, Agarose gel ...

  14. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...greater than the one that can be achieved using pharmacological or electrophysiological methods (Deisseroth...depression-like behaviors: a critical analysis." Pharmacology Biochemistry and Behavior 122 (2014): 144-157...circuits in response to drug exposure." Neuropsychopharmacology 41.10 (2016): 2424. PubMed PMID: 26934955...applications of optogenetics." Current protocols in pharmacology 75.1 (2016): 11-19. PubMed PMID: 27960028. Van...

  15. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...phagocytosis (ADCP), Fcγ receptors on macrophages bind IgG antibodies. The macrophages then engulf and digest the...antibody-dependent cellular phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV Parkinsonism Pietro De Camilli 187767 MAC_C_CSF1R CSF1R MAC Hereditary diffuse leukoencephalopathy Markku... GFP U6, Cbh Spinocerebellar ataxia 6 Harold MacGillavry 131791 pHAGE-N-FLAG-HA-TBK1 TBK1 Flag, HA CMV...

  17. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...chromosomal DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally...lower temperatures and allows the replication machinery room to come in and get busy making copies.   ...incompatible because they will compete for the same machinery, creating an unstable and unpredictable environment...

  18. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...organelles, such as mitochondria, as well as macromolecules like actin and myosin, and enzymes for transmitter...two pathways also use different cytoskeletal machineries to facilitate transport: retrograde transport...interaction with uptake or intracellular transport machinery. Addgene offers viral preparations of AAV2-retro...serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex, Neurosci Res 93, 144-157. PubMed...

  19. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab found that mismatch... developed a system in which the cellular MMR machinery is disrupted via a dominant negative mutation ...intended edit further weakened recognition by MMR machinery and improved editing efficiency. The lab also ...

  20. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...Bhang, a research investigator in the In Vivo Pharmacology group at Novartis Oncology, is an exciting new...her new role as an investigator in the In Vivo Pharmacology group at Novartis Oncology, Bhang has been working... Bhang is a research investigator in In Vivo Pharmacology at Novartis Oncology and is particularly interested...

  21. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ... which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...compacted it becomes. This prevents the cellular machinery from physically interacting with the genes in ... transposon methylation underlies the mantled somaclonal variant of oil palm." Nature 525.7570 (2015):...

  22. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ... it has been challenging to introduce CRISPRi machinery into diverse bacteria species. Existing protocols...bacteria with a chromosomal copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient...contain a chromosomal copy of the RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the...

  23. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...and departments will have qPCR machines available for use. ddPCR machines are not as commonly available.... PMID: 21554069. Haridan, U. S., Mokhtar, U., Machado, L. R., Abdul Aziz, A. T., Shueb, R. H., Zaid, ...

  24. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...doesn't require homology-directed repair (HDR) machinery, and could thus be used in non-dividing cells....convert adenine to inosine, which the translational machinery treats like guanine. This RNA editor would permit...as assessed using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral...

  25. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...This post was contributed by Rory Macneil, founder of Research Space. There are many types of electronic...equal. Many thanks to our guest blogger, Rory Macneil!  Rory is founder and CEO of Research Space, which...in Edinburgh. You can follow Rory on Twitter @rory_macneil.     Additional resources on the Addgene blog...

  26. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...temporal control than is achieved by traditional pharmacologic methods. A number of strategies have been developed...where to activate the pathway than traditional pharmacology techniques allow. Multiple photoswitches can...doi.org/10.1038/nn.2495 Marshel, J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja...

  27. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... Modification. Pharmaceutics, 13(5), 750. https://doi.org/10.3390/pharmaceutics13050750 Murlidharan, G...serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. Neuroscience Research, 93, 144...

  28. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...resulting in processing by the endogenous RNAi machinery. Alternatively, endogenous miRNA precursors can...initial state of the dsRNA upon activating the RNAi machinery (long dsRNA, typically hairpin RNA, or artificial...-2005-837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based ...

  29. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...2. Fluorescence Activated Cell Sorter (FACs) A machine that sorts cells according to whether or not they... deflect them into appropriate containers. The machine is useful for distinguishing cell populations that...PMCID: PMC2569002. 3. Egen, Jackson G., et al. "Macrophage and T cell dynamics during the development and...

  30. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...were included in the CGBE. They also developed a machine learning model capable of predicting editing outcomes... CRISPRi screens, target-library analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425...with sequence context preference predictable by machine learning methods. Nature Communications, 12(1),...

  32. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Neuroligin-1 Rat Mouse IgG2a 114555 Anti-MMACHC [N289/16R] MMACHC Human Mouse IgG2a 114556 Anti-SUR1 [N212...

  33. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...cheer, and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis (@AlexDainis...

  34. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing...

  35. Science as Art

    Type
    Blog Post
    ...non-academic people.” Daniel went on to discuss his work: “Macro photography has been my passion for a while - a ...teacher of dental radiography, had focused his x-ray machine not on teeth, but on blossoms, thus revealing the...

  37. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. His work experience is in...

  38. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This...

  39. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...studies showed that iTOP functions through macropinocytosis. D’Astolfo et al. tested iTOP in a series...Protein Delivery Techniques: Introduction of macromolecules into cultured mammalian cells by osmotic lysis...

  40. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...
Showing: 41 - 80 of 253 results