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We narrowed to 71 results for: codon optimized

Showing: 51 - 71 of 71 results

  1. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...amplified for cloning into one of three Zeamays codon-optimized Cas9-containing binary vectors. The system ...follow these same assembly principles, but they’re optimized for different purposes.   Gersbach Lab multiplexing...destination vector based on pCAMBIA. Cas9 is plant-optimized, and plasmids are available for both monocot and... paper at Addgene. 10. Port, Fillip, et al. “Optimized CRISPR/Cas tools for efficient germline and somatic...Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE Recombineering." Sci Rep. 6:19452. PubMed...

  3. Lentiviral Prep Service

    Type
    Collection
    ...Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...

  4. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...pathogenic alleles, or introduce a premature stop codon to render an RNA nonfunctional. In their quest to...directed evolution across the full ADAR2DD to further optimize cytidine deaminase activity. While RESCUE can ...

  6. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...20805792. 7. Stynen B., Van Dijck P., Tournu H. A CUG codon adapted two-hybrid system for the pathogenic fungus...channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions...

  7. Open Enzyme Collection

    Type
    Collection
    ... (Link opens in a new window) designed and codon-optimized the Open Enzymes parts for expression in Escherichia...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...develop an optimized photoactivatable CRISPR gene activation system, but settled on a different optimal fusion...researchers tested a variety of combinations to optimize both fusion proteins. The best performing combination...fusion protein combination. The optimized LACE system consisted of: 1) CIBN-dCas9-CIBN, where CIBN is the... would add the PCK upon reaching the amber stop codon, TAG. (To learn more about site-specific incorporation...

  10. Plasmids for Stem Cell Research

    Type
    Collection
    ...p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive... Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast reprogramming to cardiomyocytes...transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Elife...

  13. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Designing shRNA Oligos for pLKO.1 B.1 Determine the optimal 21-mer targets in your gene B.2 Order oligos compatible... F.1 Recommended materials F.2 Determining the optimal puromycin concentration F.3 Protocol for lentiviral...Designing shRNA Oligos for pLKO.1 B.1 Determining the Optimal 21-mer Targets in your Gene Selection of suitable...silencing: Starting at 25nt downstream of the start codon (ATG), search for 21nt sequences that match the ...plasmid to obtain the ratio that gives you the optimal viral production. d. Create a master mix of FuGENE...located in the “Appendix”. F.2. Determining the Optimal Puromycin Concentration Each cell line responds...Addgene strongly recommends that you determine the optimal puromycin concentration for your cell line before...

  14. Molecular Biology Reference

    Type
    Guide
    ...nucleotides termed a codon. As there are only 20 natural amino acids and 64 codon combinations each amino...multiple codons code for each amino acid. There are 20 amino acids plus a start and stop codon. Below you...ochre) *AUG is the most common start codon. Alternative start codons include CUG in eukaryotes and GUG in...amino acid is encoded for by multiple codons. Plasmids and Recombinant DNA Technology Techniques in chemistry...field of recombinant DNA technology have been optimized for their use of studying and manipulating genes...origin of replication, and MCS. They are small and optimized to help in the initial cloning of a DNA fragment... thymine in RNA molecules. Every 3 nucleotides (codons) in a DNA sequence encodes for an amino acid. The...

  15. CRISPR Guide

    Type
    Collection
    ... an optimized gRNA for your application (see: Plan Your Experiment ). In addition to an optimized gRNA... frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the targeted...structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between PE ...compared to HDR. Prime editing requires more optimization and typically has lower efficiency (< 50%) than.... gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity...shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA repair...sequence ORF O pen R eading F rame; The translated codons that make up a gene PAM P rotospacer A djacent ...

  16. Optogenetics Guide

    Type
    Guide
    ...been created via genetic point mutations, codon optimization, and chimeric fusion of domains from two ...engineered with enhancements such as: Human codon optimization Example: Halo Increased photocurrent amplitude...

  17. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...synaptic glutamate response. They generated variants optimized for different applications (v82 has higher affinity...indicator (GEVI) called JEDI-2P (Liu et al. 2022) optimized for two-photon imaging. They use a unique and ...multiparameter screen for accelerated development and optimization of soluble genetically encoded fluorescent biosensors...variety of imaging conditions (155 μs and <0.1 °C in optimal conditions). While B-gTEMP itself is a significant...viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt    Aggarwal A, et al. Glutamate indicators ...

  18. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...lab modified the nuclear localization signals and codon usage BE4 to create BE4max and AncBE4max with a ...cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nature Biotechnology...

  19. CRISPR Guide

    Type
    Guide
    ... an optimized gRNA for your application (see: Plan Your Experiment ). In addition to an optimized gRNA... frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the targeted...structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between PE ...compared to HDR. Prime editing requires more optimization and typically has lower efficiency (< 50%) than.... gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity...shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA repair...sequence ORF O pen R eading F rame; The translated codons that make up a gene PAM P rotospacer A djacent ...

  20. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...different retroviral vectors using a novel, CoDOn-Optimized gene for chimeric GALV envelope. Viruses , ...

  21. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags GFP FP other Flag GST His TEV... Pickrell 131792 pHAGE-TBK1-C-FLAG-HA TBK1 STOP codon CMV ALS Alicia Pickrell 131889 pDONR221_SLC1A3 SLC1A3...
Showing: 51 - 71 of 71 results