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Showing: 21 - 40 of 1087 results

  1. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    Blog Post
    ...DNA concentration. It’s important to consider the advantages and disadvantages of each method and when...using UV absorbance to quantify DNA Fluorescence dyes Another way to quantify DNA would be to use fluorescent...electrophoresis Quantifying DNA using capillary electrophoresis is similar to quantifying DNA using gel ...fragments are quantified first before larger fragments. Figure 1: The quantification of a RNA ladder...Some last reminders about quantifying DNA In choosing your DNA quantification method there’s many things...UV absorbance is one of the most common ways to quantify DNA. This method involves measuring the absorbance...your DNA prep and whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally...

  2. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    Blog Post
    ...-type. However, variants such as the P.1 (Gamma variant) and the B.1.351 (Beta variant) were much less...B.1.351 variant neutralization escape The lab saw that the B.1.351 variant was very resistant to vaccine...differed depending on the variants (Garcia-Beltran et al., 2021). Antibodies from individuals who received...vaccine could neutralize many variants, including the B.1.1.7 (Alpha variant), B1.1.298, and B.1.429 (Epsilon...on the variant. The lowest levels of cross-neutralization occurs in the P.1 and B.1.351 variants tested...circulating variants. This allowed us to create pseudoviruses that represent lineages and variants that are... distinct from SARS-CoV-2 and its variants. Yet, the B.1.351 variant, which has only 9 different mutations...

  3. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post
    ...Many Benefits of Recombinant Antibodies Recombinant antibodies offer numerous advantages over polyclonal...Fvasconcellos.     How are Recombinant Antibodies Made? To make recombinant antibodies, you first need to know...definition of recombinant antibodies are essential for good reproducibility. Non-recombinant antibodies are notorious...I predict a similar revolution for antibodies. Recombinant antibody technology makes it easy for scientists...the scientific toolbox. What are Recombinant Antibodies? Antibodies are used by scientists around the...Bottom) Recombinant monoclonal antibodies are encoded in plasmids and produce a single antibody recognizing...recognizing one epitope.   Recombinant antibodies are monoclonal antibodies that are generated in vitro ...

  4. CRISPR Antimicrobials

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    Blog Post
    ... infected by antibiotic resistant bacteria. Even when researchers develop new antibiotics, the onset of...there is no antibiotic strategy available to target only virulent or antibiotic resistant bacteria, and... fight against antibiotic resistance. Advantages of CRISPR over traditional antibiotics The potential ...CRISPR antimicrobials successfully resensitized a beta-lactam resistant population to the antibiotic, despite... the antimicrobial must reach essentially every cell or else small pools of antibiotic resistant cells...bacteria that are antibiotic resistant. Once these bacteria have been killed, antibiotics can be used to ...resensitize bacteria to antibiotics. A lysogenic phage infects antibiotic resistant bacteria, causing them...

  5. Antibodies 101: Validation

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    Blog Post
    ...does not guarantee recognition of the target antigen. You want to make sure that your antibody binds to... a great antibody signal in the non-mutant line but no signal in the mutant line! Recombinant Strategies... an antibody.  Antibodies and Reproducibility First, let’s review why validation is so important. Antibodies...expression of antibody-independent approaches to your antibody-dependent approach. Antibody-independent ...by your antibody. You perform immunocytochemistry on these mutant cells as well as a non-mutant line and...means, such as through antibody databases like Antibodypedia or through your antibody provider(s) if they...choose an antibody? Check out our Selecting an Antibody blog post! Learn more about Antibodies and their...

  6. Antibodies 101: ChIP

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    Blog Post
    ...s blog Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction...DNA Incubate your antibody, or antibodies, with your samples, and then use an antibody capture method such...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene... ChIP works by using antibodies to capture protein:DNA complexes with antibodies specific to your protein...mask the epitopes antibodies recognize. It is therefore best to either select antibodies validated for ChIP...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP. If...your antibody:protein:DNA complexes and finally (5) isolated the DNA from your antibody:protein:DNA complexes...

  7. Antibodies 101: Immunoprecipitation

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    Blog Post
    ...primary antibody interfering with downstream assays. This is particularly advantageous if the antibody used...specificity of anti-epitope antibodies make this approach appealing, but the disadvantages must be carefully...Addgene's antibody posts  Resources on Addgene.org: Find plasmids encoding recombinant antibodies at ... the same basic strategy: immobilize the antibody or antibody-target protein immune complexes, wash away... this method, the free antibody is incubated with the protein mix and antibody-target protein immune complexes... the process of anchoring an antibody, often called the capture antibody, to agarose or magnetic beads...and isotype of their primary antibody. To circumvent this, a recombinant protein, Protein A/G, was developed...

  8. Antibodies 101: Isotypes

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    Blog Post
    ...Why are antibody isotypes important? To your immune system, an antibody’s isotype is important because...by its heavy-chain constant region (see Antibodies 101: Introduction to Antibodies for a refresher). There...Transportation of antibodies to places they can’t reach on their own. The antibody’s heavy-chain constant domains... the same constant heavy chain domain as your primary antibody but doesn’t bind your antigen of interest...valency of 10 antigens. An extra constant domain replaces the hinge region in IgM antibodies. IgM has no...blog Learn about the advantages and disadvantages of using monoclonal antibodies Read about other techniques...that you can use antibodies for in our Intro to Antibodies post Check out our Antibodies 101: What is an...

  9. New Antibody Animation!

    Type
    Blog Post
    ...about antibodies? Our latest animation is here to help! Join Abi as they explain what antibodies are, ...role in the immune response, and the different antibody isotypes found in mammals.      Subscribe to...

  10. The Twelve Days of Antibodies

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    Blog Post
    ...online Antibody Guide!    🎶On the fourth day of Antibody, Addgene gave to me…  A recombinant Anti-Arl13b...me… A recombinant Anti-PSD-95!    🎶On the seventh day of Antibody, Addgene gave to me… Antibodies developed...day of Antibody, Addgene gave to me…  A recombinant Anti-DYKDDDDK!   🎶On the ninth day of Antibody, Addgene...neuroscience antibody collection!   🎶On the tenth day of Antibody, Addgene gave to me…  A recombinant Anti-APP...day of Antibody, Addgene gave to me…  An Antibodies 101 eBook!  🎶On the second day of Antibody, Addgene...Addgene gave to me…  A recombinant anti-GFP!    🎶On the third day of Antibody, Addgene gave to me…  An online... of Antibody, Addgene gave to me… The Addgene Antibody Data Hub!    🎶On the sixth day of Antibody, Addgene...

  11. Antibodies 101: Conjugation

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    Blog Post
    ...commercially conjugated antibodies available, that never guarantees the antibody you need will be conjugated... the methods for antibody conjugation can be quite variable from antibody to antibody or batch to batch...research antibodies. Lysine residues are found throughout antibody sequences, up to 80 per antibody (Mueller...abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide conjugates... conjugate antibodies directly to beads for purification purposes.  Coupling up - antibody conjugation...mechanisms. Most take advantage of chemical groups intrinsically present on antibodies, such as the primary...disulfide bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies post-translationally...

  12. 6 Tips for Grant Writing

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    Blog Post
    ...improve your grant writing and hopefully increase your success rate. 1. Submit your grant early! First... 2. Originality is essential for grant success The two most important things reviewers will be looking...in writing small travel grants to attend conferences and a seed funding grant for a proof-of-principal...ones. With grant applications there’s going to be an element of luck involved so it’s important not to throw...www.assaygenie.com. No matter what facet of academia you are in, grant writing can be the lynch pin that results in your...First off, and perhaps the most important bit of advice I can suggest, is to give yourself plenty of time..., give it back to you, and for you to make the relevant alterations before submitting - handing them your...

  13. Corralling and Cornering the Goldilocks Antibody

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    Blog Post
    ...their recombinant antibodies. Since recombinant antibodies are a newer technology, it is important to show... blog Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ...and cost-effective) antibody for my experiment – the “goldilocks antibody.” Antibodies are one of the most... the antibody crisis, one must corral and corner the goldilocks antibodies. A goldilocks antibody is highly... popular and useful antibodies are, they are in a midst of a crisis. The “antibody crisis” came to light...light as antibodies became commercialized, revealing the unreliability of antibodies. There are three main...ability – i.e., the antibody can no longer bind to the target). Unreliable antibodies cause research that...

  14. Antibodies 101: Affinity Reagents

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    Blog Post
    ....org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant antibody plasmid...as is antibody recognition, since antibody-specific antibodies bind to and recognize the constant region... (the antibody region that binds to the antigen) from existing antibodies. Each cleaved antibody will ...from camelid antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an...co-crystalize, a major advantage over antibodies (Kwon, 2019).  Single-domain antibodies (sdAbs), sold under...colors.  To antibody or not to antibody?  That is the question… or is it? This post isn’t meant to convert...101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction to Antibodies Additional resources...

  15. Our New Antibody Mascot is....

    Type
    Blog Post
    .... Name Our New Antibody Friend We need your help naming our latest mascot, an antibody joining us on ...following along on our journey to name our new antibody mascot, who's joining us in preparation for our...for Abi as they help us prepare for our upcoming antibody launch in late March!      *If you submitted ...Blugene and Aavery's newest friend, our Addgene antibody mascot, here to help us with our upcoming affinity...suggestions here or by using the hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we...

  16. Antibodies 101: Epitope Availability

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    Blog Post
    ...s blog Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...collection of antibodies in your lab. Perhaps you’re even in that mythical place where antibodies are well-...hides the epitope that the antibodies used to recognize the protein, your antibody will no longer be effective...immunohistochemistry assay, you may want to rule out monoclonal antibodies that have been validated solely...Validation  Once you’ve selected an antibody, or multiple antibodies, you’ll need to validate them for ...which antibodies are suitable for which assays and conditions when you’re building your antibody library...

  17. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...single antibody to visualize their target. These antibodies are generated against a specific antigen and ...two antibodies: a primary and secondary. For indirect IF, the primary antibody targets the antigen of ...each antibody performs differently and some antibodies impact the performance of other antibodies. But...secondary antibodies on hand to mix and match with a larger collection of primary antibodies. This strategy...target and sample type. Primary Antibody This is the first antibody applied to your sample, which will...incubation time. Secondary Antibody Unsurprisingly, this is the second antibody you apply, which binds ...experimental context.  Choosing antibodies for your assay The antibodies you choose for your experiment...

  18. Antibodies 101: Flow Cytometry

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    Blog Post
    ...expression, measured using antibody conjugated fluorophores Fluorescent antibodies get around the challenges...staining a primary antibody binds to a target and a fluorochrome-conjugated secondary antibody binds to the ...are low as multiple secondary antibodies can bind to a primary antibody thus amplifying the signal.   ...should also include a secondary antibody control in which the primary antibody has been omitted from the staining... about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody plasmids...Have you ever wanted to measure expression of your protein of interest in a single cell? Or perhaps, ...surface receptor are stained with a fluorescent antibody against the receptor and analyzed on a flow cytometer...

  19. Luminescent Imaging with Nano-lanterns

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    Blog Post
    ... developed Nano-lantern technology. Nano-lanterns contain a Renilla luciferase variant fused to an FP;...contexts. Improving Nano-lanterns In 2015, Takai et al. expanded the Nano-lantern color palette to include...producing a constant supply of substrate. Using this compound to excite the Nano-lanterns, Takai et al... Nano-lanterns (eNL) described in Suzuki et al. improve the brightness of previous Nano-lanterns by 2-...appropriate for use with Nano-lantern technology. Multicolor imaging using Nano-lanterns requires a linear unmixing... signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used a linear...explained further in Takai et al. Nano-lanterns and ready-made Nano-lantern biosensors are available from Addgene...

  20. Antibodies 101: Affinity Tags

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    Blog Post
    ...Resources on Addgene.org Addgene antibodies Addgene’s antibody guide Antibody protocols  Resources on the...especially if downstream antibody detection is desired… and yes, we have an antibody for that!   Fig....of these tags also have commercially available antibodies, resins, beads, and other purification aids readily...are trying to purify The expression system If you want the tag to remain on your protein for downstream... to remove the cleaved tag from solution. MBP antibodies are available for detection and/or purification...purification of MBP-tagged proteins. The antibody is an alternative to amylose resin and also provides a visual...you are having solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often ...
Showing: 21 - 40 of 1087 results