...opportunity to study it and see what happens when technological change happens really fast,” Thompson says. ... groups have generated robust tools that have practical applications in monitoring the accidental or intentional...essential and complementary tools in a time where biological engineering and synthetic biology is expanding...

...while the wet-lab resources, like constructs or biological material, can be deposited in the specific repositories...

...an experimental system comes with fewer ethical and logistical challenges than does working with animals...grown to include local school districts, professional education associations, and local organizations such...Education & Outreach Specialist for the Arabidopsis Biological Resource Center and the Center for Applied Plant...University. Established in 1991, the Arabidopsis Biological Resource Center (ABRC) is one of two global stock...preference for using animal systems to demonstrate biological concepts - and you have yourself a plant publicity...publicity crisis. The Arabidopsis Biological Resource Center (ABRC) set out to remedy that with the establishment...conducting teacher workshops, or staffing a booth at local science festivals, ABRC gets the word out about ...

...activate essentially any gene,” she says. The technical leap from mutating and deactivating a gene or ...Zhang’s lab describe just such a system, which they call CRISPR/Cas9 Synergistic Activation Mediator or SAM... and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating...distinct effector domains that work together synergistically, modeled after natural transcription activation...GeCKO, a lentiviral CRISPR library enabling genome-scale, knockout screening). As the Zhang lab's Nature ... is robust, specific, and can facilitate genome-scale gain-of-function screening when combined with a ...demonstrated in a paper in Cell that they could typically achieve 90-99% knockdown with minimal off-target...

...specific and much faster than any genetic or pharmacological manipulations on cells, and has potential use... platform for visualizing RNA production and localization Article contributed by Tyler Ford  Listen to...set of tools that can be used to visualize RNA localization in mammalian cells. These tools consist of cobalamin-binding...platform at Addgene. Braselmann E, et al. Nature Chemical Biology. 2018 PubMed PMID: 30061719. New tools...expression vector (pRSETB). Fabritius A, et al. Cell Chemical Biology 2018. PubMed: PMID 30220597. ACE system...

...reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a protein...intracellular calcium levels within the cell. When levels of Ca2+ are high in the cell, calcineurin dephosphorylates...attractive drug target. Calcineurin reporters allow scientists to measure calcineurin dynamics and can serve...identify new antifungals that target calcineurin. Current calcineurin gene reporters are based on the bacterial...screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical issues, ...genetic tool for labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe...

...Gordon Fishell Calcium GCaMP5 genetically encoded calcium sensor Optimization of a GCaMP calcium indicator ... Javier García-Sancho Calcium Genetically encoded calcium indicators for optical imaging (GECOs), including...Schreiter Calcium GCEPIA1-SNAP ER for sensing ER calcium A genetically encoded ratiometric calcium sensor...Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded Calcium Indicators (Constitutive or... Loren Looger , GENIE Project Calcium GCaMP3 genetically encoded calcium sensor Imaging neural activity...GCaMP calcium indicators. Nat Methods. 2009 Dec;6(12):875-81. Loren Looger Calcium NEMO calcium sensors...intracellular calcium. Nat Commun. 2021 Dec 9;12(1):7159. Dorus Gadella Calcium Teal genetically encoded calcium...

...-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting 51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato...AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium sensor Ting 83899 pAAV-mDlx-GCaMP6f-Fishell...element Calcium sensor Fishell 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate...jRGECO1a Calcium Sensor Kim , GENIE 100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn jRGECO1a Calcium Sensor ...pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium sensor Kim 104491...Cre-dependent Calcium sensor Kim 104493 pGP-AAV-syn-FLEX-jGCaMP7b-WPRE Syn GCaMP7b, Cre-dependent Calcium sensor...Cre-dependent Calcium sensor Kim 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG GCaMP7f, Cre-dependent Calcium sensor...

...dendrite localization tag) Lin Tian 135420-AAV1 pAAV-syn-FLEX-jYCaMP1s Yellow protein calcium sensor expressed...pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P... pAAV-hSyn-JEDI-2P-Kv-WPRE Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P... application of innovative tools enabling large-scale real-time interrogation and monitoring of brain ...pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer Viviana ...

...antibody’s constant region - specifically, it is determined by a region called the “heavy chain constant ...reagents are molecular tools used in the lab to specifically and tightly bind to other proteins. Antibodies...interest. The protein that an antibody recognizes is called an antigen, while the specific binding site is ...referred to as an epitope. Production Antibodies are typically produced in one of three different methods: in...independently manipulated. Antibodies produced this way are called recombinant antibodies. Recombinant antibodies ...are responsible for binding the antigen, and are called the antigen binding fragments (Fab). The tips of...the Y structure and the lower part of the arms is called the constant region, and is shown in dark blue ...

...Anti-CALB2/calretinin [L122/68R-2b] CALB2/calretinin Human Mouse IgG2b 206642 Anti-CALB2/calretinin [L122...Anti-CALB2/calretinin [L122/68R-1] CALB2/calretinin Human Mouse IgG2a 206691 Anti-CALB2/calretinin [L122...Anti-Calbindin [L109/39R] Calbindin Human Mouse IgG2a 177455 Anti-Calbindin [L109/57R] Calbindin Human...Anti-Calretinin [L122/68R] Calretinin Human Mouse IgG2a 177469 Anti-Calretinin [L122/6R] Calretinin Human...Human Mouse IgG2b 206635 Anti-CALB1/calbindin [L109/39R-2b] CALB1/calbindin Human Mouse IgG2b 206636 Anti-Homer1L...Human Mouse IgG1 206686 Anti-CALB1/calbindin [L109/39R-1] CALB1/calbindin Human Mouse IgG1 206687 Anti-Homer1L...220384 Calretinin [L122/110R] Calretinin Human Mouse IgG2a 220385 Calretinin [L122/117R] Calretinin Human...

..., including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors GCaMP8...soma-GCaMP6 Twitch2B Yellow Calcium Sensors jYCaMP1 axon-jYCaMP1 Red Calcium Sensors CaMPARI jRCaMP1 jRGECO1...viral service encoding biosensor tools, including calcium sensors. Viral...jRGECO1 sRGECO Far-Red/NIR Calcium Sensors HaloCaMP1a HaloCaMP1b NIR-GECO Acetylcholine Sensors iAChSnFR..._ACh3.0 Syn GRAB_ACh3.0 none Constitutive 9 Li Calcium Sensor: GCaMP8f 162376 pGP-AAV-syn-jGCaMP8f-WPRE...CAG jGCaMP8f none Constitutive 1, 9, rg* Looger Calcium Sensor: GCaMP8s 162374 pGP-AAV-syn-jGCaMP8s-WPRE...CAG jGCaMP8s none Constitutive 1, 9, rg* Looger Calcium Sensor: GCaMP8m 162375 pGP-AAV-syn-jGCaMP8m-WPRE...

... of identical bases. Browse Plasmids: Prime Edit Large Scale Edits CRISPR can generate large-scale genetic...takes place during the S and G2 phases. Chemically or genetically inhibiting genes involved in NHEJ or upregulating...properties, often called a reeling mechanism. The targeted strand is cleaved non-specifically by Cas3 along...immunoprecipitation (enChIP) using CRISPR. Biochemical and Biophysical Research Communications . 439 (1), 132... generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect...gRNAs will be expressed in the same cell. This is called multiplexing , and increases the likelihood that...DNA with no homology elsewhere in the genome. Realistically, most gRNA targeting sequences will have additional...

... beta CALC2, CGRP-II, CGRP2, FLJ30166 CALCR calcitonin receptor CRT, CTR, CTR1 CALCRL calcitonin receptor-like...CD88 CALCA calcitonin-related polypeptide alpha CALC1, CGRP, CGRP-I, CGRP1, CT, KC, MGC126648 CALCB calcitonin-related...beta-2-microglobulin - CALR calreticulin CRT, FLJ26680, RO, SSA, cC1qR CANX calnexin CNX, FLJ26570, IP90,...formerly 2B), catalytic subunit, alpha isoform CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B PPP3CB protein phosphatase...guanyl releasing protein 1 (calcium and DAG-regulated) CALDAG-GEFI, CALDAG-GEFII, MGC129998, MGC129999...

...mitochondrial localization 101124 pSNAPf-H2B Control Plasmid SNAP-tag Control for nuclear localization 101123...mitochondrial localization 101125 pCLIPf-NK1R Control Plasmid CLIP-tag Control for cell surface localization 101127...surface localization 101131 pACP-GPI Control Plasmid ACP/MCP-tag Control for cell surface localization 101128...Control plasmids with a recognizable sub-cellular localization (membrane, nucleus, etc.) are also available...-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine...visualization of complementary protein function or localization. ACP and MCP tags ACP- and MCP-tags are small...SNAP-tag Control for endomembrane (CaaX box) localization 101129 pSNAPf-Cox8A Control Plasmid SNAP-tag...

...interacting domain (NID) a nuclear localization signal (not essential for localization) the C - t erminal D omain... opens in a new window) ClinicalTrials.gov - a list of Rett syndrome clinical trials (Link opens in a ...window) PMID: 16615965 Leonard et al. 2017. Clinical and biological progress over 50 years in Rett syndrome...months of age. A defining feature, with onset typically between 18 to 30 months, is regression of previously...of Rett syndrome is currently based on specific clinical diagnostic criteria, defined as the presence of...MECP2 gene. Causative mutations for Rett syndrome typically arise spontaneously, although in rare cases Rett...of MECP2 is to regulate gene expression either locally or globally. The MECP2 protein contains several...

...collection contain fluorescent protein tags whose localization you can monitor in live cells (beware of artifacts...staining, and RNA transcript tags to monitor RNA localization. Using these plasmids you’ll hopefully get a...coat protein binding sites Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence... that found in pZS*12-MS2-GFP ) to track RNA localization of your gene of interest once its appropriate... inducible) MBP, 6x His, signal sequence for localization to the periplasm David Waugh Empty lactose/IPTG...

...small biomolecules or other physiological intracellular processes with genetically encoded fluorescent biosensors...mammals. Regulate Biological Activity Use fluorescent proteins to modulate biological activity, like transcription...Plasmids by Category Empty Backbones Understand the localization and/or function of your protein of interest ...Plasmids | Guide Use small molecules to activate genetically engineered cellular receptors that affect signalling...signalling pathways within cells. Subcellular Localization Determine where your protein of interest resides...Resources FPbase from Talley Lambert at Harvard Medical School Fluorescent Biosensor Database designed ...

...Yale have made improvements to this system by genomically recoding E.coli (Lajoie et al., Science 2013)...of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1* ( 188537 ) is a ROP minus plasmid...of interest by mass spectrometry in a technique called serine-oriented library/kinase-library reactions...Barber et al, Biochemistry 2018). Iterative Synthetically Phosphorylated Isomers (iSPI). This new resource...proteomics search algorithms, proteomics phosphosite localization algorithms, or any method confounded by phosphosite...WW2 domain) References & Protocols Iterative Synthetically Phosphorylated Isomers (iSPI) described in: ...: A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics. Gassaway...

...engineering, offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful...to destroy the phages upon re-exposure. More specifically, specialized Cas proteins snip foreign DNA into...editing with CRISPR is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be...robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology development has occurred...programmable platform well-suited for various biological and translational technologies. Biotechnology...agricultural and therapeutic applications. Despite the ethical controversies surrounding non-research applications... it’s adaptable to almost any model system or biological topic, and you don’t need to be an expert to ...