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We narrowed to 658 results for: CHI

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Showing: 21 - 40 of 658 results

  1. Antibody Guide

    Type
    Collection
    ... Special considerations One type of ChIP assay, called native ChIP, does not use cross-linking and instead...accessible to antibodies. Detection of sdAbs is usually achieved by conjugation of a signaling molecule directly...can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to distinguish...detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody is done through...Immunoprecipitation (IP) Chromatin immunoprecipitation (ChIP) Visualization - used to visualize protein location...either X-ray film in a darkroom or an image capture machine. Special considerations The primary antibody must...immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) allow you to isolate specific proteins or protein...

  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NCerulean - Zebrafish/Xenopus/Worm/Sea urchin mCerulean N1 - Mammalian...Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NeGFP - Zebrafish/Xenopus/Worm/Sea urchin pKT0128 - Yeast Expression...Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus/Worm/Sea urchin pET mCitrine LIC cloning...Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NmOrange - Zebrafish/Xenopus/Worm/Sea urchin mOrange-N1 - Mammalian...Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NmCherry - Zebrafish/Xenopus/Worm/Sea urchin pET mCherry LIC cloning...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Angew Chem Int Ed Engl. 2018 Jun 27. Tetsuya Kitaguchi ATP Variants of ATP sensor iATPSnFR1.0 and iATPSnFR1.1.... PLoS One. 2014 Jun 24;9(6):e100252. Tetsuya Kitaguchi cAMP (cyclic AMP) Red fluorescent protein-based...imaging. Sci Rep. 2017 Aug 4;7(1):7351. Tetsuya Kitaguchi cAMP (cyclic AMP) Fluorescent sensor of cyclic...PDE5alpha. ACS Sens. 2017 Jan 27;2(1):46-51. Tetsuya Kitaguchi cGMP (cyclic GMP) FlincG3 (GFP-based cGMP sensor...PLoS One. 2014 Jun 5;9(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic...Anal Chem. 2019 Apr 2;91(7):4821-4830. Tetsuya Kitaguchi Glucose Red fluorescent glucose indicators for...ratio changes in different cellular compartments. Biochim Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519...

  4. CRISPR References and Information

    Type
    Collection
    ...pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF, 108 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF,...fungi, protists, and viruses. Developed by the Joachim Wittbrodt and Juan L. Mateo (Link opens in a new...sequences. Organisms include human, mouse, rat, chicken, frog, zebrafish, fly, worm, Japanese rice fish...design and cloning; injection protocol pU6-BbsI-chiRNA ; phsp70-Cas9 PDF, 109 KB Orkin and Bauer Protocol...

  5. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...particular gRNA. In the enChIP (engineered DNA-binding molecule-mediated ChIP) system, catalytically inactive... used for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing ...researchers have expanded chromatin immunoprecipitation (ChIP) to allow purification of any genomic sequence specified...

  6. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...MDH-related research into their teaching laboratories. They encourage instructors teaching different types of courses...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and...development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor future ...

  7. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ... Description Category PI 157970 pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin...subretinal injection. It was identified by John Chiorini and characterized by Shannon Boye’s lab. AAV44.9...Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26(3):165-76. ...in future publications, please acknowledge John Chiorini and Shannon Boye and cite Boye, et al. 2020. Novel...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...EB1 EGFP Ken-Ichi Takemaru 122871 GFP-hCCDC11 Centriolar satellites CCDC11 EGFP Ken-Ichi Takemaru 54234...Mykytyn *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...Harrison *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...Fanning *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein... Pagano *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...

  9. Deisseroth INTRSECT Collection

    Type
    Collection
    ...nucleus accumbens medial shell and behavioral switching. Neurochemistry International 129:104482. PubMed...opens in a new window) Yu K, Ahrens S, Zhang X, Schiff H, Ramakrishnan C, Fenno L, Deisseroth K, Zhao ...in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons in ...in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1...hypothalamus-habenula circuit controls aversion. Mol. Psychiatry 24(9):1351-1368. PubMed (Link opens in a new ...

  10. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... and its associated proteins or RNAs through ChIP (enChIP) or biotin-streptavidin pulldown (CAPTURE.) ...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun . 439...for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product....Myers RM. 2015. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res . 25(10)...

  11. E11 Bio PRISM Collection

    Type
    Collection
    ...tissue, these protein barcodes allow for efficient machine-learning segmentation of neurons and proofreading...been used in expansion microscopy workflows to achieve high-resolution optical imaging down to individual... microscopy and reconstruction of neurons with machine learning. Adapted from Park & Sheridan et al. (... Park, S.Y., Sheridan, A., An, B., Jarvis, E., Lyudchik, J., Patton, W., Axup, J.Y., Chan, S.W., Damstra...

  12. Viral Vector Guides and Plasmids

    Type
    Collection
    ...cells in an organism and use the host cellular machinery to produce more virions. B) Viral plasmids have...to packaging cell lines and use their cellular machinery to generate viral vectors for research and gene...viral genome contains only the necessary genes to achieve this goal. When a virus recognizes a host cell,...genome into the cytoplasm and hijacks the host’s machinery to produce more viruses. In this way, viruses ...

  13. Zebrafish Plasmid Collection

    Type
    Collection
    ...zebrafish research. China Zebrafish Resource Center (CZRC) - A resource center based in China focused on collecting...Sean Megason Lab Zebrabow - Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish...lineages and cell types in the vertebrate brain - Alex Schier Lab Other Tools of Interest A few examples of popular...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Lynda Chin 21691 pLenti6/V5/DEST-VPS35 VPS35 V5 CMV Parkinson's Lynda Chin 21827 TLS 1: hTLS.pBSKS...Gabriele Kaminski Schierle 108866 pT7-7 aSyn C141 SNCA T7 Parkinson's Gabriele Kaminski Schierle 108867 pET29b...Kaminski Schierle 108868 pLJM1 EGFP-Tau MAPT GFP CMV Parkinson's, FTD Gabriele Kaminski Schierle 109321 ... mScarlet CMV ALS Kyoko Chiba 184826 pSN838 KIF5A mScarlet CMV ALS Kyoko Chiba 184827 pSN526 KIF5A mScarlet...Strep polH ALS Kyoko Chiba 184832 pSN840 KIF5A mScarlet Strep polH ALS Kyoko Chiba 184894 pMRX-IZ-ANXA11...-2b] - Chimeric FXN CMV Friedreich ataxia James Trimmer 227032 Anti-Pink1 [N4/15-2b] - Chimeric PINK1 ...pMXs-mPKR-FLAG-IG EIF2AK2 Flag LTR VWM disease Seiichi Oyadomari 101848 pTO_CHMP2B_LAP CHMP2B LAP CMV ...

  15. Tetracycline Inducible Expression

    Type
    Collection
    ...simplicity, other promoter elements and transcription machinery are not shown. Tetracycline Off (Tet-Off) The ...doxycycline (Zhou et al., 2006). Tet-On 3G systems can achieve >10,000-fold induction of the gene of interest....cell lines or transgenic animals can be made to achieve greater control over expression with flexibility...Galla, M., Maetzig, T., Baum, C., Loew, R., & Schiedlmeier, B. (2011). Retroviral and transposon-based ...

  16. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... or 20 matching nucleotides, the second binds to mcherry with either 0, 11, 18, or 20 matching nucleotides...possible to study the effect of the amount and stoichiometry of enzymes. The pLC97 plasmid allows for quick...

  17. Antibody Plasmid Collection

    Type
    Collection
    ...Fab fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments...antibodies as Fab' fragments, isotype-switched chimeric antibodies, and Fc-silent mutants. Functional ...

  18. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...-Puro 73039 Expresses Nluc and copGFP from the chicken beta-actin CMV enhancer pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro... the EF1 promoter while pICPIS-CB contains the Chicken-beta actin promoter with CMV enhancer. pAdx-CMV-LacZ...polyA pICPIS-CB 73356 AdenoX shuttle vector with chicken-beta actin CMV enhancer promoter SV40 polyA Addgene...

  19. Viral Production

    Type
    Collection
    ...ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method. Titering by the...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...Transfer Plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...

  20. TALEN Plasmids and Kits

    Type
    Collection
    ...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...is driven by the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...
Showing: 21 - 40 of 658 results