We narrowed to 821 results for: EED

...process may need to be optimized for individual assays. Clear cell debris by sedimentation. Proceed with IP..., an antimicrobial agent, can block some amines needed for conjugation reactions and should be removed...chosen. Signal Amplification The amount of signal needed from a specific antibody can vary depending on ...category, including an outline of the basic steps needed for each application, important controls, and any...samples to make proteins available. Tissue samples may need additional processing steps, such as sonication....signal levels. In ChIPseq, an input control will need to be collected before immunoprecipitation. Antibody...from. Then consider the following questions: Do you need polyclonal or monoclonal antibodies? Will you be...

...human transmembrane transporters. Feedback Please send your feedback to the Resolute Partnership using...RESOLUTE Plasmid Collection Plasmids Pooled Libraries Feedback Resources Solute carrier proteins (SLCs) are the...using their RESOLUTE repository feedback form (Link opens in a new window) . If you have further questions...

...requirements for a CRISPR knockout assay are simple. You’ll need a Cas enzyme and a gRNA specific to the gene of ...Cas9-gRNA complex binds a putative DNA target, the seed sequence (8–10 bases at the 3′ end of the gRNA targeting...) will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...Mismatches between the target sequence in the 3′ seed sequence inhibit target cleavage, while mismatches...NHEJ directly ligates the break ends without the need for a homologous template. Both NHEJ and HDR have... the depositing lab. After amplification, you’ll need to use next-generation sequencing (NGS) to verify...matches that of the original library. Next, you’ll need to generate lentivirus containing the entire library...

...affordable prices. While our repository serves the needs of many researchers, scientists sometimes require... our current in-stock capabilities. To meet that need, our Packaged on Request service lets scientists...Troubleshooting If your answer isn’t in the Help Center or you need more assistance with troubleshooting, we want to...

...the tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru...plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what you would...

...modifications in endogenous loci with unprecedented speed and specificity. Figure 2: An overview of CRISPR...a DSB at the target genomic locus. Repair will proceed through NHEJ or HDR depending on the experimental... model system or biological topic, and you don’t need to be an expert to see results! Getting Started ...

...designed based on the target site sequence (20bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence...designed based on the target site sequence (30bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence... based on the target site sequence (21-22bp) and need to be followed on the 3' end by NGRRT or NGRRN PAM...

...transactivator may be needed. Or, when inducing a potentially toxic protein you may need extremely minimal...window) Resnitzky, D., Gossen, M., Bujard, H., & Reed, S. I. (1994). Acceleration of the G1/S phase transition...

...with each shipment. If you can't find the prep you need, try Addgene's AAV Packaged on Request service. ...search of viral preps and filter by the serotype needed....

...multiple orders would need to be placed through our website. Every order will need to be completed independently...

...community resource that is growing! We welcome your feedback and suggestions for new antibodies to add to the...official views of the National Institutes of Health. Need Some Help? Browse some of our other resources to...

...spectrophotometry using a NanoDrop spectrophotometer. If needed, the sample is adjusted by diluting or concentrating... shipping conditions will be shown in your cart. Need Some Help? Browse some of our other resources to...

... an Account: Before placing your order, you will need to create an account and affiliate it with your ...has not ordered from Addgene in the past, we will need to verify your account. We will notify you by email...

... to produce recombinant AAV vectors, researchers need to use three plasmids: A transfer plasmid (or cis... helper genes (E4, E2a, and VA), eliminating the need for a separate helper plasmid during AAV production...

...for targeted gene mutation. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for..., preserves, reproduces, and distributes diverse seed and other stocks of Arabidopsis thaliana and related...

...techniques, as well as the plasmids and protocols needed for using them, are described below. Read our CRISPR...gene of interest is currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9...

...editing at a given locus. To use the tool, you'll need Sanger sequencing reads from PCR amplicons that ... Program for designing optimal gRNAs. Provides feedback on number of potential off-targets, target's genomic...

...backbones are plasmids containing only the elements needed for replication in the host cell and are designed...drive expression of your favorite gene, you will need a plasmid with a promoter that will be functional...

...Already know what gene or regulatory element that you need a luciferase reporter for? Browse or use the search...flexibility in emission colors and imaging without the need for an illumination source. BRET can be particularly...

...preparation. Systemic Delivery AAV Capsid Choice Need help deciding which capsid to choose? The following...plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what you would...