We narrowed to 959 results for: LAT;

...humans. Then, one of them heard about our online platform, called Crowdfight, and filed a request for help...Crowdfight-COVID19 Crowdfight was born as a collaborative platform to help researchers working on COVID-19. Its origins...feeling helpless about the situation. But a few days later we had an idea. And this idea rapidly grew and became... became Crowdfight COVID-19, an online platform to connect researchers working on COVID-19 with scientists... designers and other professionals, joined the platform; while they are a minority, their help has been... to push certain projects. The idea behind the platform was simple: any person working on COVID-19 could...simpler and the volunteers helped with things like translations, bibliographic searches, etc.     Expanding...

...tagless protein purification as well as reversibly regulation of endogenous signaling activity in cells.  Let...spatiotemporal resolution than pharmacological or genetic manipulation of the signaling pathway.  As a proof-of-principle...Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling...results demonstrate that OptoNBs can be harnessed to regulate signaling pathways such as the Ras/Erk pathway...

...Anastasia continued profiling samples, and years later, several whole genome sequencing projects were launched...

...!   Epigenetics nation stuck with methylation correlation  In the 1980s, a lot of things were cool... slightly smaller population, something else was also suddenly cool: DNA methylation. In 1983, Andrew ...these studies report correlations without clearly testing whether a certain methylation state causes a condition...cause demethylation?  Dividing cells depend on DNA methyltransferase 1 (DNMT1) to copy methylation marks...site. So, when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked by ...the original methylated strand is diluted to undetectable amounts: ergo, DNA demethylation! With optimization...allowing one to study the causal relationship between DNA demethylation and gene expression (Sapozhnikov...

...our information updated, export controls and regulations are complex and may change suddenly. Please email...

... Congenital Disorders of Glycosylation (APCDG) and an international platform for CDG research, named the...NFIB- and NFIC-related human syndromes. As therapies are developed for specific NFI-related genetic disorders...on rare diseases through the BeHEARD Award. Congratulations to Paula Videira, Michael McMurray, and Richard...Addgene to advance their research on diseases of glycosylation, arginine mutagenesis, and neural development...development. Paula Videira: Congenital disorders of glycosylation Paula Videira is an Assistant Professor at the...CDG&Allies-PPAIN).  Congenital disorders of glycosylation (CDG) are a group of rare inherited metabolic...Immunological aspects of congenital disorders of glycosylation (CDG): a review. J. Inherit. Metab. Dis. 39,...

...KI-RAS, KRAS1, KRAS2, NS3, RASK2 LAT linker for activation of T cells LAT1, pp36 LCK lymphocyte-specific ...ADRM1 adhesion regulating molecule 1 ARM1, GP110, MGC29536, Rpn13 AP3B1 adaptor-related protein complex...polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related sequence... IREL RFX5 regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated...kinase, regulatory subunit 1 (alpha) GRB1, p85, p85-ALPHA PIK3R2 phosphoinositide-3-kinase, regulatory subunit...JULF2, KPG_004, NOP9 PF4 platelet factor 4 CXCL4, MGC138298, SCYB4 PF4V1 platelet factor 4 variant 1 CXCL4L1...

...system that enables researchers to manipulate the genome with relative ease. Over the past decade, researchers... Select Your Desired Genetic Manipulation Different genetic manipulations require different CRISPR components... donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing...Does the resulting population need to be identical (clonal) or is a mixed population okay? Does the edit...modifications . Cell Population The first step of validation is determining the type of cell population you need ...you will likely need a clonal population. Additionally, clonal populations are useful for determining if...edited alleles. A clonal population is not always required, and a mixed population can be sufficient for ...

...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and is...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...modifying the methylation state of cytosines in a gene’s promoter or by inducing histone acetylation or demethylation...through increased H3K27 acetylation TET1 — activation through cytosine demethylation DNMT3A — repression ...repression through cytosine methylation MQ1 — repression through cytosine methylation with improved kinetics LSD1...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing sequence...

...generate the new phosphorylated protein. Protein Phosphorylation Protein phosphorylation is one of the most...orthogonal translation system to make phosphorylated proteins with one or more phosphorylation sites (Pirman...questions surrounding serine phosphorylation by adding this phosphorylated amino acid to the genetic code... serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library ). The phosphorylation sites (“... most abundant forms of posttranslational modification in cells and research into its many roles in protein... , and an optimized phosphoserine orthogonal translation system, pSerOTS-C1* (V70) (Plasmid #188537) .... with the optimized phosphoserine orthogonal translation system, SepOTSλ (Plasmid #68292) , they were ...

...mRFP-Clc Clathrin-coated vesicles Clathrin light chain mRFP Ari Helenius 54040 mEmerald-Clathrin-15 Clathrin-coated... vesicles Clathrin light chain mEmerald* Michael Davidson 79997 pmiRFP703-Clathrin Clathrin-coated vesicles...vesicles Clathrin light chain miRFP703 Vladislav Verkhusha 21741 Clathrin-LCa-EYFP Clathrin-coated vesicles...vesicles Clathrin light chain EYFP Xiaowei Zhuang 164462 Clathrin-mGreenLantern Clathrin-coated vesicles...vesicles Clathrin light chain mGreenLantern Gregory Petsko 184667 pClathrin-miRFP670nano3 Clathrin-coated vesicles... IX Membranes Palmitoylation tdTomato Connie Cepko 22479 FUmGW Membranes Palmitoylation EGFP Connie Cepko...Membranes Palmitoylation sequence from GAP43 EGFP Connie Cepko 61099 Lck-GFP Membranes Palmitoylation sequence...

...produces a colored product that accumulates over time which is relative to the amount of enzyme present...quantify relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific ... one type of antibody. Monocultures of B cells isolated from animals immunized as described above are ... regions of the antibody to be independently manipulated. Antibodies produced this way are called recombinant... used while suspended in the original media or isolated and placed in a buffer. Antibody Structure Figure...antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain...instead of modified antibodies and are not closely related to antibodies. Monobodies - Monobodies are synthetic...

...enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many cancers....RRAGB RRAGC RRAGD Ras-related GTP binding Raptor Also known as RPTOR; Regulatory associated protein of...arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation...determine the nutritional state of the cell and regulates the switch from anabolic to catabolic processes...Enhanced signaling through either mTORC1 or mTORC2 correlates with poor prognosis in many cancers, including...Akt and MAPK/ERK, both of which are commonly dysregulated in cancer. Loss of the tumor suppressor p53 ... caused by inactivating mutations in negative regulators of mTORC1. The hyperactivation of mTORC1 in cancer...

...(ras related) RALBP1 RalA binding protein 1 RALGDS Ral guanine nucleotide dissociation stimulator RAPGEF...have an active (GTP) and inactive (GDP) state, regulated by the addition and removal of a phosphate group...GTPases play prominent roles in signaling pathways regulating cell migration, survival, adhesion, growth, and...differentiation and survival. Ras is essential for regulating the growth of the cell, however when mutations...for clarity and does not indicate a specific relationship. The content and map for this page were generated...EIF4EBP EIF4EBP1 EIF4EBP2 EIF4EBP3 Eukaryotic translation initiation factor 4E binding protein ERBB2 Erb-b2...

...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135630-AAV9 pAAV-S5E2...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135630-PHPeB pAAV-S5E2...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135631-AAV1 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135631-AAV9 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135631-PHPeB pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135632-AAV1 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135632-AAV9 pAAV-S5E2...

...NTR 2.0/MTZ-based cell ablation system that allows sustained cell loss and ablation of previously...localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly model potential cancer...embryo and larvae, it is easy to culture large populations that are amenable to genetics, high-throughput...emerged as a model system for human disease and translational research, including the study of cancer, inflammation... enabling CRISPR/dCas9-interference (CRISPRi) modulation of enhancers and non-coding RNAs in zebrafish...organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure Lab Simultaneous...droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics in vivo. Bimolecular fluorescence...

... of treatments and cures for Rett Syndrome and related MECP2 disorders. The RSRT has a comprehensive, ...diagnostic criteria, defined as the presence of relatively normal early development, regression with a loss...The MECP2 protein is a global transcriptional regulator of thousands of genes and studies have suggested...is not clear, however, the ability to bind to methylated DNA and recruit known co-repressors or other ...modifiers suggests the major function of MECP2 is to regulate gene expression either locally or globally. The...NID demonstrating the importance of binding to methylated DNA and the recruitment of the NCoR complex for... MECP2 null alleles or common mutations that recapitulate Rett syndrome in humans. MECP2 knockout rats...

...available at Addgene. Amyotrophic Lateral Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes referred...individuals develop late-onset AD. There is no clear association between a gene and late-onset AD. It is ... between a person’s thirties and mid-sixties, or late-onset, where symptoms appear during or after a person's...APOE), such as the ε4 allele, are a risk factor for late-onset AD. Many more genes have been associated with... tag gene variants for Alzheimer's disease and related dementias with plasmids from the iPSC Neurodegenerative...human iPSC-derived neurons with a CRISPRi-based platform . (Tian et al. Neuron. 2019) See More AAV Viral...Enhancer Collection to target different cell populations in many brain regions. See More iPSC Differentiation...

...interneurons under the control of the E2 regulatory element Viral manipulation of functionally distinct interneurons...calcium sensors for imaging activity in neuronal populations and microcompartments. Nat Methods. 2019 Jul;...GCaMP6f Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator. Neuron...calcium indicator for in vivo imaging of neuronal populations at single-cell resolution. PLoS Biol. 2025 Apr...) Improved methods for marking active neuron populations. Nat Commun. 2018 Oct 25;9(1):4440. Eric Schreiter...ER-targeted CEPIA1er ER-mitochondria tethering by PDZD8 regulates Ca 2+ dynamics in mammalian neurons. Science. ...sensitivity Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo. Nat ...

...through both phosphorylation and acetylation, which disrupt its interaction with negative regulators, increase...Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related cysteine peptidase CASP9...allow it to bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional...for clarity and does not indicate a specific relationship. p53 Pathway Color is used for clarity and does... does not indicate a specific relationship. The content for this page was generated with the help of .... CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3 Cyclin B1, 2, or 3 Cyclin...proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-...