...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...MaCPNS1, MaCPNS2, CAP-B10, CAP-B22 University of Florida Serotype Testing Panel for the Eye and Brain For...

...nucleotides. Together, these plasmids allow to explore the whole range of expression levels for these .... Each point represents a single cell, and each color represents the population obtained with one CRISPR...

...watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal, mitochondrial, peroxisomal...window) — Learn how to get started with a CURE, explore MDH projects, find teaching resources and protocols...

...individual links to each gene page are provided below. Color is used for clarity and does not indicate a specific... with the help of David Sabatini . mTOR Pathway Color is used for clarity and does not indicate a specific...

...Geobacillus sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium sp. Neisseria...plasmid collection is organized by application and by color. External Resources European Saccharomyces Cerevisiae...

...below. Color is used for clarity and does not indicate a specific relationship. p53 Pathway Color is used...

...translational technologies. Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling of chromosomal loci in human ...

...pyogenes Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol Marraffini...

...mL RNase A TE or water-saturated phenol-chloroform Chloroform 100% ethanol or isopropanol 90% ethanol ...: Perform phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction removes...Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the...volume of chloroform to the recovered aqueous DNA layer. Repeat steps 2-4. Note: Phenol-chloroform is a hazardous...aqueous DNA sample. Pro-Tip Water-saturated phenol-chloroform can be used if TE-saturated is not available....without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant after step 6 and...

...56 °C for 30 min. 25 mM chloroquine diphosphate Dissolve 0.129 g of chloroquine diphosphate salt into 10...slowly add hydrochloric acid until the solution clears. Check the pH of the solution Use hydrochloric acid ... Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear MW 25,000 Da Microcentrifuge....X 10 cm tissue culture dish, Corning 430167 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone... will be basic and will need adjustment with hydrochloric acid first. Pro-Tip The pH of this solution ...add 10 mL fresh DMEM complete containing 25 µM chloroquine diphosphate and incubate ~5 h For 10 mL of DMEM...DMEM complete, add 10 µL of 25 mM chloroquine diphosphate. Dilute 18.9 µg of DNA into 500 µL of Opti-Pro...

...are also included: using Isopropanol or Lithium Chloride. This protocol was adapted from (Link opens in...Water-saturated Phenol 2 M Sodium Acetate pH 4 Chloroform/Isoamyl alcohol (49:1) 75% Ethanol RNase-free...for precipitation step, Option A) 7.5 M Lithium Chloride (for precipitation step, Option B) Glycogen (Optional...phenol, mix thoroughly by inversion. Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) and then shake vigorously...(s). You can use either Isopropanol or Lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...aqueous layer. Incubate at -20°C for 1 hour. Lithium Chloride (Option B): LiCl selectively precipitates RNA ...from the homogenized sample(s). Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) per 1 mL of TRIzol® used...

... add hydrochloric acid until the solution clears. Check the pH of the solution. Use hydrochloric acid ... Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear MW 25,000 Da Heat-inactivated...Corning 430167 15 mL conical tubes, VWR 21008-216 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone... will be basic and will need adjustment with hydrochloric acid first. Pro-Tip The pH of this solution ...mL conical and an appropriate amount of 25 mM chloroquine to the conical so that the final concentration...transfection mixes are added) will be 25 uM of chloroquine. After the incubation, add the DNA:PEI-Max mix...mix to the conical containing the media and chloroquine and mix well. Gently aspirate the media out of...

...marking of mouse neurons with multiple distinct colors Mammalian Sanes See article Multibow constructs...dependent tdtomato expression. See article for more colors. Mammalian Zeng 24334 hsp70l-loxP-mCherry-STOP-...

... Optogenetics The lab of Michael Davidson from Florida State University has contributed their comprehensive...

...Pro-Tip We batch label our plates with colored marker - particular colors correspond to particular antibiotics...extract 10.0 g peptone from casein 10.0 g sodium chloride 12.0 g agar-agar 1 L Sterile H 2 O Sterile plates...5 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL (dissolve in EtOH) 25 µg/mL Coumermycin...

...periodically to determine when the desired color change has occurred. The color will change from yellow to blue ...optimize experimental conditions. When the desired color change has occurred, gently remove the plate seal...generate a standard curve . If you don’t see any color change or dose response, your antibody concentration...

...anti-glypicans that accurately detect glypican presence and explore glypican function, providing a reliable and accessible...

... cells. Other microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins...

...add hydrochloric acid until the solution clears. Check the pH of the solution and use hydrochloric acid... pH 8.5 + 60 mL of 5 M Sodium Chloride + 4 mL of 1 M Magnesium Chloride Close the bottle and mix by inverting...

...George N, Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell surface proteins. J Am Chem Soc...