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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 379 results for: Lor;

Showing: 21 - 40 of 379 results

  1. Fluorescent Protein Guide

    Type
    Collection
    ...Should I Use? Blog: How to Choose a FP For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent...and Digital Imaging Olympus Fluorescent Protein Color Palette...

  2. AAV Viral Preps

    Type
    Collection
    ...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...MaCPNS1, MaCPNS2, CAP-B10, CAP-B22 University of Florida Serotype Testing Panel for the Eye and Brain For...

  3. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...nucleotides. Together, these plasmids allow to explore the whole range of expression levels for these .... Each point represents a single cell, and each color represents the population obtained with one CRISPR...

  4. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal, mitochondrial, peroxisomal...window) — Learn how to get started with a CURE, explore MDH projects, find teaching resources and protocols...

  5. mTOR Pathway

    Type
    Collection
    ...individual links to each gene page are provided below. Color is used for clarity and does not indicate a specific... with the help of David Sabatini . mTOR Pathway Color is used for clarity and does not indicate a specific...

  6. Microbiology Resources

    Type
    Collection
    ...Geobacillus sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium sp. Neisseria...plasmid collection is organized by application and by color. External Resources European Saccharomyces Cerevisiae...

  7. p53 Pathway

    Type
    Collection
    ...below. Color is used for clarity and does not indicate a specific relationship. p53 Pathway Color is used...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...translational technologies. Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling of chromosomal loci in human ...

  9. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...mL RNase A TE or water-saturated phenol-chloroform Chloroform 100% ethanol or isopropanol 90% ethanol ...: Perform phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction removes...Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the...volume of chloroform to the recovered aqueous DNA layer. Repeat steps 2-4. Note: Phenol-chloroform is a hazardous...aqueous DNA sample. Pro-Tip Water-saturated phenol-chloroform can be used if TE-saturated is not available....without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant after step 6 and...

  10. General Transfection

    Type
    Protocol
    ...56 °C for 30 min. 25 mM chloroquine diphosphate Dissolve 0.129 g of chloroquine diphosphate salt into 10...slowly add hydrochloric acid until the solution clears. Check the pH of the solution Use hydrochloric acid ... Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear MW 25,000 Da Microcentrifuge....X 10 cm tissue culture dish, Corning 430167 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone... will be basic and will need adjustment with hydrochloric acid first. Pro-Tip The pH of this solution ...add 10 mL fresh DMEM complete containing 25 µM chloroquine diphosphate and incubate ~5 h For 10 mL of DMEM...DMEM complete, add 10 µL of 25 mM chloroquine diphosphate. Dilute 18.9 µg of DNA into 500 µL of Opti-Pro...

  11. Kit Free RNA Extraction

    Type
    Protocol
    ...are also included: using Isopropanol or Lithium Chloride. This protocol was adapted from (Link opens in...Water-saturated Phenol 2 M Sodium Acetate pH 4 Chloroform/Isoamyl alcohol (49:1) 75% Ethanol RNase-free...for precipitation step, Option A) 7.5 M Lithium Chloride (for precipitation step, Option B) Glycogen (Optional...phenol, mix thoroughly by inversion. Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) and then shake vigorously...(s). You can use either Isopropanol or Lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...aqueous layer. Incubate at -20°C for 1 hour. Lithium Chloride (Option B): LiCl selectively precipitates RNA ...from the homogenized sample(s). Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) per 1 mL of TRIzol® used...

  12. Lentivirus Production

    Type
    Protocol
    ... add hydrochloric acid until the solution clears. Check the pH of the solution. Use hydrochloric acid ... Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear MW 25,000 Da Heat-inactivated...Corning 430167 15 mL conical tubes, VWR 21008-216 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone... will be basic and will need adjustment with hydrochloric acid first. Pro-Tip The pH of this solution ...mL conical and an appropriate amount of 25 mM chloroquine to the conical so that the final concentration...transfection mixes are added) will be 25 uM of chloroquine. After the incubation, add the DNA:PEI-Max mix...mix to the conical containing the media and chloroquine and mix well. Gently aspirate the media out of...

  14. Cre-lox system

    Type
    Collection
    ...marking of mouse neurons with multiple distinct colors Mammalian Sanes See article Multibow constructs...dependent tdtomato expression. See article for more colors. Mammalian Zeng 24334 hsp70l-loxP-mCherry-STOP-...

  15. Pouring LB Agar Plates

    Type
    Protocol
    ...Pro-Tip We batch label our plates with colored marker - particular colors correspond to particular antibiotics...extract 10.0 g peptone from casein 10.0 g sodium chloride 12.0 g agar-agar 1 L Sterile H 2 O Sterile plates...5 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL (dissolve in EtOH) 25 µg/mL Coumermycin...

  16. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...periodically to determine when the desired color change has occurred. The color will change from yellow to blue ...optimize experimental conditions. When the desired color change has occurred, gently remove the plate seal...generate a standard curve . If you don’t see any color change or dose response, your antibody concentration...

  17. AAV Production in HEK293 Cells

    Type
    Protocol
    ...add hydrochloric acid until the solution clears. Check the pH of the solution and use hydrochloric acid... pH 8.5 + 60 mL of 5 M Sodium Chloride + 4 mL of 1 M Magnesium Chloride Close the bottle and mix by inverting...

  18. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...CRISPR to label multiple loci in distinct colors. To create a colorful Cas9 toolbox, Ma et al. turned to SpCas9...nucleome. CRISPRainbow: Scaling up to 6 colors + white Color Hairpin Blue MS2-MS2 Green PP7-PP7 ...number of limitations. Since color is specified by PAM sequence, each color requires a different Cas9 ortholog...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...track multiple genomic loci over time using all the colors of the CRISPRainbow. Figure 1: CRISPRainbow...chromosome-specific loci in the U20S cancer cell line./ Each color represents a gRNA targeting a specific chromosome...different fluorescent protein to create three distinct colors. The specificity of these orthologs is key: since...

  20. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...later. Single Colors Single color controls contain, as the name suggests, a single color from your antibody... single color, fluorescence minus one (FMO), and isotype. You should always have single color controls...single color control for every fluorophore you intend to use in your panel. Using single color controls...from your single color controls may not be accurate for your samples. The single color controls should ... flow plots for a single color control. A) A flow plot showing a single color control for the APC channel...can bind to any primary antibody you add. Single color controls are used to help you set up your voltages...achieved by adjusting your voltage using your single color controls (Figure 1A), instead of using your actual...
Showing: 21 - 40 of 379 results