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Showing: 21 - 40 of 467 results

  1. p53 Pathway

    Type
    Collection
    ...suppressor activated by cellular stresses like DNA damage, oxidative stress, and nutrient deprivation. p53...cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose from its observed molecular...Gadd45 GADD45A GADD45B GADD45G Growth arrest and DNA-damage-inducible; alpha, beta, or gamma IGF-BP3 Insulin-like...2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific DNA binding protein 2, 48kDa p53 Tumor...

  2. Zebrafish Plasmid Collection

    Type
    Collection
    ...aggregates in real time. Live imaging of astroctyes - Kelly Monk Lab. Live imaging tools to study the development...high-throughput screening and non-invasive live imaging. A standard toolbox of genetic methods, such as...Lipid droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics in vivo. Bimolecular...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Doench and Root 3rd 4 78,637 Broad GPP Humagne Set C and Humagne Set D 172650 (Set C) 172651 (Set D) Knockout...Inhibition Mouse Weissman 3rd 5 10 107,415 214,830 DNA Damage Response MKOv4 Library 140219 Knockout Human J....

  4. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...-95 Human Mouse IgG2a 177440 Anti-Pan-MAGUK [K28/86R] Pan-MAGUK Human Mouse IgG2a 177441 Anti-Kvbeta1.2...TrpC6 Human Mouse IgG2a 211591 Anti-Pan-MAGUK [K28/86R-2b] Pan-MAGUK Human Mouse IgG2b 211592 Anti-Pan-Na...IgG2a 227070 Pan-MAGUK (Homo sapiens) recombinant mouse monoclonal antibody. Pan-MAGUK Human Chimera: M...

  5. Antibody Production

    Type
    Collection
    ...blue. Protein band intensities are measured using ImageJ software and a standard curve is generated. The...concentration is calculated from the standard curve. Using ImageJ software, the band intensities of both the antibody...

  7. Deisseroth INTRSECT Collection

    Type
    Collection
    ...transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image from Fenno et al., 2014. INTRSECT INTRSECT (intronic...opens in a new window) Wick ZC, Tetzlaff MR, Krook-Magnuson E. 2019. A novel population of long-range inhibitory...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...gRNAs that bind fluorescent proteins can be used to image genomic loci in live cells. RNA targeting : Cas13...EH, Weissman JS, Qi LS, Huang B. 2013. Dynamic imaging of genomic loci in living human cells by an optimized...

  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ...LY57, MGC111051, SLP-65, SLP65 BTK Bruton agammaglobulinemia tyrosine kinase AGMX1, AT, ATK, BPK, IMD1...ILT5, LIR-3, LIR3, MGC138403, PIRB LYN v-yes-1 Yamaguchi sarcoma viral related oncogene homolog FLJ26625...cytoplasmic domain, (semaphorin) 5B FLJ10372, KIAA1445, SEMAG, SemG SEMA6A sema domain, transmembrane domain (...C19orf10 chromosome 19 open reading frame 10 EUROIMAGE1875335, IL25, IL27, IL27w, R33729_1, SF20 C3 complement...K-FGF, KFGF FGF5 fibroblast growth factor 5 HBGF-5, Smag-82 FGF6 fibroblast growth factor 6 HBGF-6, HST2 ...

  10. Brain Initiative Collection

    Type
    Collection
    ...GRAB_rDA-mut in neurons Yulong Li 158757-AAV8 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter... GCaMP6f. Edward Boyden 158757-AAV9 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter...

  11. Chemogenetics Guide

    Type
    Guide
    ...activation Magnus et al., 2019 PSAM Q79G,Q139G Gly PSEM 22S Cl - influx Neuronal inhibition Magnus et al.,...activation Magnus et al., 2011 PSAM L141F,Y115F Gly PSEM 89S Cl - influx Neuronal inhibition Magnus et al.... 792, uPSEM 817 Cl - influx Neuronal inhibition Magnus et al., 2019 PSAM4 5HT3 Varenicline, uPSEM 792,... PSEM 89S Na + , K + influx Neuronal activation Magnus et al., 2011 Browse Addgene's collection of PSAM...activated through chemogenetic or optogenetic routes. Image courtesy of Ute Hochgeschwender . Table 3. Descriptions...Huntington's Disease Pathogenesis. iScience , in press. Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, and Sternson...96. PMID: 21885782 (Link opens in a new window) Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez...

  12. Antibody Guide

    Type
    Guide
    ...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...a substrate kit. Image the membrane using either X-ray film in a darkroom or an image capture machine....reaction can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...antibody, incubate, and wash. Preserve sample and image using a microscope. Special considerations Many ...

  13. CRISPR Guide

    Type
    Guide
    ...repress target genes, purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA...antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9-VPR...Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including the simplicity...detect multiple targets simultaneously. CRISPR imaging can visualize chromatin and RNA dynamics in live...RNA-binding proteins (RBPs). Multicolor CRISPR imaging can be achieved by using orthogonal dCas9s (e.g... stability and amplifies the signal for better imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent...Cas13 fusions have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: ...

  14. Cloning

    Type
    Guide
    ...gene of interest (YGOI) into a recipient plasmid. (Image from Plasmid 101: Restriction cloning ) Back to ...Gateway® Recombination Cloning Gateway cloning (Image from Plasmid 101: Gateway Cloning ) Gateway® cloning...gateway cloning. Back to Top TOPO® Cloning TOPO (Image from Plasmid 101: TOPO Cloning ) Toposiomerase based...chewed back by the 5' exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top ...your next cloning project. Golden Gate Cloning (Image from Plasmid 101: Golden Gate Cloning ) Back to ...

  16. Depositor Collections

    Type
    Collection
    ...Science Plasmid Collection New England BioLabs Cell-Imaging Plasmid Collection Botman-Teusink Yeast FP Collection...

  17. MAPK Plasmids

    Type
    Collection
    ...PRKM15, RK, SAPK2A, p38, p38ALPHA MAPK15 ERK7, ERK8 *Image is licensed under the GFDL. Source: Wikipedia, with...

  20. Antibody Plasmid Collection

    Type
    Collection
    ... the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their applications...
Showing: 21 - 40 of 467 results