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We narrowed to 154 results for: N-pac

Showing: 21 - 40 of 154 results

  1. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...the pattern AA(N 19 ). If no suitable match is found, search for NAR(N 17 )YNN, where N is any nucleotide...and incubate at 37°C, 5% CO 2 for 24 hours. Day 5: n. Harvest media from cells and pool with media from...control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral particles. pMD2.G ...particles. Note: pLKO.1 can also be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG...tubes VWR: #87003-290 Note: pLKO.1 could also be packaged using pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert...transfection: 1 μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl... may want to vary the ratio of shRNA plasmid, packaging plasmid, and envelope plasmid to obtain the ratio...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...created, which contain a unique asymmetric spacer "NNNTANNN", where "N" indicates which bases may vary from .... I. Recombination between loxP sites. Sternberg, N. and Hamilton, D. 1981. PubMed PMID: 6276558. 2. Site-specific...recombinase of bacteriophage P1. Sauer, B. and Henderson, N. 1988. PubMed PMID: 2839833. PubMed Central PMCID:...repeats separated by an 8-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives... double-strand DNA break occurs within the core spacer of each loxP site and the two strands are ligated...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E. coli Zhang N/A N/A N/A - The protospacers...3rd N/A N/A Retroviral Mouse Genome-wide CRISPR Knockout Library 104861 Knockout Mouse Teichmann N/A (...Mitochondrial DNA (CARM) Library 82480 Knockout Mouse Xie N/A N/A 395 CRISPRa Discontinued Activation Human Weissman...Knockout T. gondii Lourido N/A 10 8,158 TUNEYALI Library 217744 Knockout Yeast Borodina N/A 1 56 Turner Lab Human...Inhibition Library v1 115927 Inhibition E. coli Bikard N/A 19 92,919 Bikard Lab EcoWG1: E. coli Genome-wide...Inhibition Library v2 131625 Inhibition E. coli Bikard N/A ∼5 21,417 Bison sgRNA Library 169942 Knockout Human...Transporter Libraries 153101-153106 Knockout Yeast Borodina N/A 1 Varies Bovine CRISPR Knockout Libraries 213927...

  4. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... S., Lee, W., Walsh, N., Iozza, K., Huang, N., Preston, G., Drouin, L. M., Jia, N., Deng, J., Hebben, ...expression at varying capacities. AAV vectors containing the AAV2 ITRs can be cross-packaged with capsids from...replication and are required for replication, packaging, and vector persistence. They work in conjunction...in the same DNA molecule as the gene that they package. In a transfer plasmid, the transgene is flanked...deleting the B-B' or C-C' arms can reduce the packaging efficiency but increase transgene expression (...Terminal Repeats (ITRs) on the Productivity, Genome Packaging, and Potency of AAV Vectors. Microorganisms, 12...Bonnin, D., Merten, O.-W., & Galibert, L. (2017). Impact of Inverted Terminal Repeat Integrity on rAAV8 ...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...dimerization. Splitting wild-type Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments...2xNLS) and the n-terminal cas9 fragment with FKBP rapamycin binding domain of mTor (Cas9(N)-FRB-NES) resulting...Split Cas9 system The Cas9 protein is composed of an n-terminal DNA recognition domain and a mostly c-terminal...genome editing. Without rapamycin treatment, the Cas9(N)-FRB-NES fragment is actively shuttled out of the ...export sequence. Treatment with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS dimerization and net...activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES). This system provides users greater temporal...for constructing fusion proteins with customizable N- and C-terminal modules   Thanks to the lab of Tomás...

  6. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...s41587-021-01133-w Pandey, S., Gao, X. D., Krasnow, N. A., McElroy, A., Tao, Y. A., Duby, J. E., Steinbeck...efficacy.   Find CHARM plasmids here!   Neumann, E. N., Bertozzi, T. M., Wu, E., Serack, F., Harvey, J. ...., Pirtle, C. P., Coffey, A., Howard, M., Kamath, N., … & Weissman, J. S. (2024). Brainwide silencing .... Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman...evolved optimizations for prime editing CHARM: A compact epigenetic silencer Recombinant antibodies for ...assembly of biotinylated SpyTag-SpyCatcher linkers and spacer proteins ending with a GFP nanobody (Figure 1)....determination. A) Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm...

  7. Kit Free RNA Extraction

    Type
    Protocol
    ...Link opens in a new window) Chomczynski P and Sacchi N, 2006 and (Link opens in a new window) TRIzol® User...Thiocyanate 25 mM Sodium Citrate, pH 7.0 0.5% (wt/vol) N-laurosylsarcosine (Sarkosyl) 0.1 M 2-mercaptoethanol...hazards for these reagents. Work in a well-ventilated space and under a fume hood when working with the volatile...

  8. CRISPR Guide

    Type
    Collection
    ... Silverstein, R. A., Amrani, N., Peng, C., Kramme, C., Savic, N., Pacesa, M., Rodríguez, T. C., Stan, ...34478496 Yarnall, M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, R. N., Lim, J., Villiger, L.,...Ter-Ovanesyan, D., Braff, J. L., Davidsohn, N., Housden, B. E., Perrimon, N., Weiss, R., Aach, J., Collins, J. ...Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N., Kim, J. Y., Chung, A....Dy, A. J., Joung, J., Verdine, V., Donghia, N., Daringer, N. M., Freije, C. A., Myhrvold, C., Bhattacharyya...Yachie, N., . . . Nureki, O. (2018). Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science...1964. PMID: 29891532 Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson,...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Sanchez, A. D., Udeshi, N. D., Svinkina, T., Carr, S. A., Feldman, J. L., Perrimon, N., & Ting, A. Y. (2018...Flexible tagging: Choose untagged or add a versatile N- or C-terminal HA tag. Selectable and stable: A puromycin... Figure 2: Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV ...needed to replicate inside a host cell but with space to insert your genetic cargo of choice. After hearing...taken them for a test drive! We validated viral packaging and strong expression after inserting EGFP and...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...acrIIA3   Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes  pCSW21...Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants on iPSC generation Deriving...fluorophores with nonoverlapping emission spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus...discrepancies, which they traced to a 9 amino acid N-terminal variation in the Klf4 isoform (KLF4s or KLF4L...pJH376 Yes   acrIIA3 Streptococcus pyogenes  pCSW24  N/A (Toxic) pJH375  No    ... expression levels; this altered stoichiometry impacted reprogramming and global gene expression patterns...

  11. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...hgtb.2011.034 Benyamini, B., Esbin, M. N., Whitney, O., Walther, N., & Maurer, A. C. (2023). Transgene Expression...  References  Aurnhammer, C., Haase, M., Muether, N., Hausl, M., Rauschhuber, C., Huber, I., Nitschko,...adenoviral helper genes are supplied on two different packaging plasmids: the pHelper plasmid containing Ad E2A... your infectious titer, since it will have more impact on your experimental outcome and planning.  Both... of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production of recombinant...resources on the Addgene blog Parts of the AAV Packaging PlasmidParts of the AAV Transfer PlasmidAAV Variables...

  12. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Terminus 79629 (N) and 79628 (C) Cv (capsid) N and C 79633 (N) and 79641 (C) NS1 (var: W98G) N and C 79635...NS2B C 79636 NS4A N 79640 NS4B C 79639 NS5 C 79632 (N) and 79631 (C) PrM N and C   Detecting...effects. The library was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses.... The Roux lab has deposited HA tagged BioID2 for N-terminal fusions and Myc tagged BioID2 for C-terminal... (< 100 bp) and large (up to 120 kb) deletions in N. benthamiana and Arabidopsis. The authors found that...be found here). To use the library, the authors packaged it into lentivirus and used the resulting lentiviral...large size of dCas9-VPR limits one’s ability to package it into lentivirus and later create stable cell...

  13. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...Personal Perspective: Plasmid (1952-1997) Cohen, Stanley N. “DNA Cloning: A Personal View after 40 Years.”Proceedings... PubMed Central PMCID: PMC2134509. Cohen, Stanley N. et al. “Construction of Biologically Functional Bacterial...plasmids and new cloning vectors such as pBR322, pACYC, and pUC were developed to provide higher copy number... discovery in the 1950s, plasmids have greatly impacted many areas of biological research and have been...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ... tag and an SV40 NLS at the N terminus, a 152-residue deletion from the N terminus of the wild-type TALE... The GoldyTALEN scaffold is truncated at both the N and C terminus and induces mutation at rates much ...into a pCS2 expression vector resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA...the T7 promoter. Truncations were introduced to the N- and C-terminus of the pTAL3 TALEN backbone, which.../mRNA synthesis vectors containing unique shorter N- and C-terminal domains (N153AA, C47AA). The reporter...Construction and Evaluation Accessory Pack Takashi Yamamoto This accessory pack contains modified pFUS array vectors...

  15. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...inevitable. Most FP fusions for FRET studies are at the N- or C-terminus of your target protein, but even these... Royal Society of Chemistry. 3. Ermolenko, Dmitri N., et al. "Observation of intersubunit movement of ...272. PubMed PMID: 9657724. 6. Kapanidis, Achillefs N., Yon W. Ebright, and Richard H. Ebright. "Site-specific...fluorescent labeling with (Ni2+: nitrilotriacetic acid) n-fluorochrome conjugates." Journal of the American ... a bit like a Fedex delivery. Your fluorophore package needs to go to the proper mailbox (i.e., a binding...that can nonspecifically soak up your fluorophore package. One important concept in FRET experimental design...synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase Mammalian ...

  16. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C. N., Avants, B. W., & Robinson, J. T. (2019). Magnetic...andrewgyork.github.io/gfp_magnetofluorescence. Karki, N., Vergish, S., & Zoltowski, B. D. (2021). Cryptochromes...https://doi.org/10.1038/nchem.2447.  Lee, H., Yang, N., & Cohen, A. E. (2011). Mapping Nanomagnetic Fields...-up studies. “We don’t have a vision of passive impact,” he says, noting that his team regularly shares...

  17. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Stachelhaus et al. (1998) References George N, Pick H, Vogel H, Johnsson N, Johnsson K. 2004. Specific labeling...PMID:9712910 Vivero-Pol L, George N, Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell... 101131 pACP-GPI Control Plasmid ACP/MCP-tag Control for cell surface localization 101128 pACP-ADRβ2 Control...backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for...

  18. An Introduction to Adenovirus

    Type
    Blog Post
    ...10400791. Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune...Foresythe, A., Huh, H. J., Bazinet, A. L., Bergman, N. H., Bull, R. L., Garcia, K. Y., Goodrich, J. S., ....1038/s41586-023-05949-1. PMID: 36996871. Tatsis, N., Fitzgerald, J. C., Reyes-Sandoval, A., Harris-McCoy..., including the E1 region). The space in the viral particle’s capacity that is made available by this ...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mCherry Ari Helenius 37533 cfSGFP2-N Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1-COXIV-COX8...Davidson 98822 Lck-mTurquoise2 Plasma Membrane Lck N-terminal lipidation signal mTurquoise2 Dorus Gadella...Gadella 98821 Lck-mScarlet-I Plasma Membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057...Membrane Mff acGFP1 Gia Voeltz 158007 pCMV-mGold-Mito-N-7 Mitochondria Cox8A mGold Francois St-Pierre 49151...Peroxisome SRL mScarlet Dorus Gadella 55146 mCherry-TOMM20-N-10 Mitochondria-Outer Membrane TOMM20 mCherry* Michael...CCDC11 EGFP Ken-Ichi Takemaru 54234 mEmerald-PLK1-N-16 Centrosome Plk1 mEmerald* Michael Davidson 54491.../ GJA1 sfGFP David Spray 54959 mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson...

  20. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...strain. In our metabolic engineering case study of N-acetylglucosamine biosynthesis, we constructed a strain...) with 4 rounds of transformation to complete the N-acetylglucosamine strain library construction in 8...cargo copy numbers. (D) The effect of gene dosage on N-acetylglucosamine concentration. Image from the Yang... clearance. It is more convenient to update the spacer(20nt) on pEcgRNA plasmid as only two 24 nt oligos... proteins Cas6, Cas7, Cas8, and the crRNA. The spacers of the crRNA can be customized as well, including...
Showing: 21 - 40 of 154 results