We narrowed to 657 results for: PPL
-
TypeCollection...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...
-
New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection...Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England Biolabs offers non-toxic...Single constructs are compatible with multiple applications (different fluorophores, biotin, bead purification...MCP-tag Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP...purchasing coenzyme A disodium salt from a commercial supplier and conjugating it to the label of their choice... -
Microbiology Resources
TypeCollection...our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology... cloning and expression systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors...Densmore Lab EcoFlex MoClo : Modular cloning for applications like recombinant protein purification and cell-free...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces... -
Luciferase Plasmid Collection
TypeCollection...advantages and disadvantages depending on the application, and different luciferases require different ... course experiments, but is too dim for some applications. NanoLucĀ® is small (19 kDa), very bright, and... available, making it ideal for a variety of applications. To learn more and plan your experiment, check...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored version... -
Tetracycline Inducible Expression
TypeCollection...tTA-Advanced Takeshi Imai Other Tet Applications Explore some other popular applications of tetracycline systems ...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated... -
Institute for Protein Innovation
TypeCollection...Antibody Description Isotype Reactivity Recommended Applications Integrin Antibodies Integrins are a family of...Antibody Description Isotype Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI has developed...Antibody Description Isotype Reactivity Recommended Applications Browse Antibody Plasmids The following plasmids... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...Bacterial Expression mApple 568 592 37 6.5 30 min Monomer mApple-N1 - Mammalian Expression mApple-C1 - Mammalian...Mammalian Expression mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-... -
Antibody Plasmid Collection
TypeCollection...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing... -
Fluorescent Protein Guide: Biosensors
TypeCollection...sensor's behavior in the target system. Use care when applying a biosensor in a novel context or modifying an...indicators based on mRuby (jRCaMP1a, jRCaMP1b) and mApple (jRGECO1a) (Constitutive or Cre-dependent) Sensitive... Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Sci Rep....Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry...robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat Chem Biol... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...the following three buffers. All buffers are supplemented with 0.001% pluronic F68 or 0.014% Tween 20.... -
Centrifugation
TypeProtocol...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed ... -
Brain Initiative Collection
TypeCollection... the human brain through the development and application of innovative tools enabling large-scale real-time.... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting 163909-AAV9 pAAV_hSynapsin_psychLight2...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Additional Resources To learn more, please... -
Fluorescence Titering Assay
TypeProtocol... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol...working in the lab. Do not eat, drink, chew gum, or apply makeup in the laboratory. Before you start your ... -
Protocol - Bacterial Transformation
TypeProtocol... take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce membrane permeability... -
Immunocytochemistry
TypeProtocol...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your... -
Zhang Lab CRISPR Page
TypeCollection... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin... -
Lentivirus Production
TypeProtocol...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow... -
Handling Plasmids from Addgene - Purifying Plasmid DNA
TypeProtocol...the plasmid DNA. Either spin to pellet the DNA or apply the solution to a column that will bind the now ... -
Plan Your Experiment
TypeCollection...as an example, but many of these principles also apply to using CRISPR in other organisms. As with most...component for each category. Genetic Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids...gene you target will depend on your specific application. Knockout : Target constitutively expressed regions...