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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 651 results for: PPL

Showing: 21 - 40 of 651 results

  1. Plan Your Experiment

    Type
    Collection
    ...Cas9 in mammalian cells, many of these principles apply to using CRISPR in other organisms. First, consider...may not be a perfect plasmid for your specific application, and in such cases, it may be necessary to customize...reagent to suit your needs. Genetic Manipulation Application Cas9 gRNA Additional Considerations Knockout ...method Expression System Components of System Application Mammalian expression vector Cas enzyme promoter...gene you target will depend on your specific application. For example: To activate or repress a target...validate your edit will depend upon your specific application. However, there are several common ways to verify...

  2. Antibodies

    Type
    Collection
    ...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

  3. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England Biolabs offers non-toxic...Single constructs are compatible with multiple applications (different fluorophores, biotin, bead purification...MCP-tag Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP...purchasing coenzyme A disodium salt from a commercial supplier and conjugating it to the label of their choice...

  4. Microbiology Resources

    Type
    Collection
    ...our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology... cloning and expression systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors...Densmore Lab EcoFlex MoClo : Modular cloning for applications like recombinant protein purification and cell-free...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

  5. Bacterial Expression Systems

    Type
    Collection
    ...cellular activity in a way that’s interesting from an applied perspective, sometimes it’s best to delete genes... Christopher Voigt When combined with pCph8 and pPLPCB(S), makes lacZ expression inducible by red light...sensor protein, pCph8 , to be used with pJT106b3 and pPLPCB(s). pJT118 31395 PcpcG2 Green Light (532 nm) Christopher...Christopher Voigt When combined with pPLPCB(S), encodes a green light responsive sensor protein that activates...

  6. Institute for Protein Innovation

    Type
    Collection
    ...Antibody Description Isotype Reactivity Recommended Applications Integrin Antibodies Integrins are a family of...Antibody Description Isotype Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI has developed...Antibody Description Isotype Reactivity Recommended Applications Browse Antibody Plasmids The following plasmids...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Bacterial Expression mApple 568 592 37 6.5 30 min Monomer mApple-N1 - Mammalian Expression mApple-C1 - Mammalian...Mammalian Expression mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-...

  8. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...

  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...sensor's behavior in the target system. Use care when applying a biosensor in a novel context or modifying an...indicators based on mRuby (jRCaMP1a, jRCaMP1b) and mApple (jRGECO1a) (Constitutive or Cre-dependent) Sensitive... Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Sci Rep....Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry...robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat Chem Biol...

  11. Brain Initiative Collection

    Type
    Collection
    ... the human brain through the development and application of innovative tools enabling large-scale real-time.... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting 163909-AAV9 pAAV_hSynapsin_psychLight2...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Additional Resources To learn more, please...

  12. Cre-lox system

    Type
    Collection
    ...60930 pPL5071_TEF1*-Cre_URA3 Cre expressed at low levels mutant TEF2 Yeast Piper 60931 pPL5608_TEF1*-Cre_TRP1...60932 pPL5606_TEF1*-Cre_ADE2 Cre expressed at low levels mutant TEF1 Yeast Piper 60933 pPL5628_TEF1*-Cre_MET15...

  13. Zhang Lab CRISPR Page

    Type
    Collection
    ... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ...advantages and disadvantages depending on the application. Firefly luciferase is considerably brighter ...luciferase-based plasmid systems for many different applications. Browse the Collection Highlights section to...respectively, for increased usefulness in in vivo applications. LuxSit-i : An artificial luciferase desined...

  15. Centrifugation

    Type
    Protocol
    ...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed ...

  16. Fluorescence Titering Assay

    Type
    Protocol
    ... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...

  18. Validated gRNA Sequences

    Type
    Collection
    ...upstream of a 5' NGG 3' PAM sequence). Which CRISPR application is this gRNA sequence compatible with? CRISPR...Resources page have been used to indicate the Cas9 application the gRNA was designed to accomplish. Validated...Gene Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens...

  20. Immunocytochemistry

    Type
    Protocol
    ...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...
Showing: 21 - 40 of 651 results