We narrowed to 653 results for: PPL
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TypeCollection...Antibody Description Isotype Reactivity Recommended Applications Integrin Antibodies Integrins are a family of...Antibody Description Isotype Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI has developed...Antibody Description Isotype Reactivity Recommended Applications Browse Antibody Plasmids The following plasmids...
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Antibody Plasmid Collection
TypeCollection...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing... -
Fluorescent Protein Guide: Biosensors
TypeCollection...sensor's behavior in the target system. Use care when applying a biosensor in a novel context or modifying an...indicators based on mRuby (jRCaMP1a, jRCaMP1b) and mApple (jRGECO1a) (Constitutive or Cre-dependent) Sensitive... Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Sci Rep....Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry...robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat Chem Biol... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...the following three buffers. All buffers are supplemented with 0.001% Poloxamer 188 or 0.014% Tween 20... -
Brain Initiative Collection
TypeCollection... the human brain through the development and application of innovative tools enabling large-scale real-time.... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting 163909-AAV9 pAAV_hSynapsin_psychLight2...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Additional Resources To learn more visit:... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...GenHunter: #Q401 FuGENE® 6 Transfection Reagent Roche Applied Biosciences: #11814443001 OPTI-MEM® serum-free ...lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen . Moffat...Terms of Use , Addgene’s Terms of Purchase , and applicable Material Transfer Agreements between the party... -
Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps
TypeProtocol...protocols that you can use for a wide range of applications, with videos for select protocols in the right-hand...preparation Antibodies Protocols for common antibody applications. Name Description Link to Video Transfection... -
Zhang Lab CRISPR Page
TypeCollection... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol... reaction with both. MgCl 2 acts to supplement the amount supplied by the buffer stock, where it is known... -
Plan Your Experiment
TypeCollection...as an example, but many of these principles also apply to using CRISPR in other organisms. As with most...component for each category. Genetic Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids...gene you target will depend on your specific application. Knockout : Target constitutively expressed regions... -
Affinity Purification of Recombinant Antibodies with Protein A or Protein G
TypeProtocol...column in a new 50 mL LoBind collection tube. Slowly apply the sample to the center of the compact resin bed...exchange and concentration with an Amicon column Apply 15 mL of PBS to the reservoir of a Amicon Ultra-... -
Western Blot
TypeProtocol...Spectrophotometer Heat block Mini gel tank chamber Power supply iBlot 2 Gel Transfer Device Roller Spatula Platform...tank and plug the electrode cords into the power supply. Run the gel at 100 V for 10–15 min or until the... -
AAV ddPCR Titration
TypeProtocol...Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes ... the BSC to be used for droplet generation are supplied with sufficient pipette tips and reagent reservoirs... -
Kit Free RNA Extraction
TypeProtocol... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating... -
Validated gRNA Sequences
TypeCollection...upstream of a 5' NGG 3' PAM sequence). Which CRISPR application is this gRNA sequence compatible with? CRISPR...Resources page have been used to indicate the Cas9 application the gRNA was designed to accomplish. Validated...Gene Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens... -
Tags and Other Markers
TypeCollection...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies... -
Neuroscience
TypeCollection...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies... -
Fluorescent Protein Guide
TypeCollection...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins and to find plasmids... -
CRISPR Plasmids - gRNAs
TypeCollection...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...upstream of a 5' NGG 3' PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR... -
Addgene Packaged on Request: Scope of Service
TypeCollection...subject to Addgene’s Terms of Purchase and the applicable material transfer agreements (MTAs) and limited...contact you directly regarding these charges, if applicable....