...replication. They contain two genes: gag and pol. The pol polyprotein encodes the reverse transcriptase and ...reading frames (ORFs) which often terminate with a poly(A). ORF2 encodes endonuclease and reverse transcriptase...

...necessary for the production of a viral particle (Gag, Pol, Tat, Rev and Env) and the transfer plasmid encoding...this vector requires only three HIV-1 genes (gag, pol, and rev) for production. Third generation lentiviral... you will find will recommend using a polycation, like polybrene (protocol) or DEAE dextran (protocol)...

...When Publishing a Scientific Manuscript by Maaike Pols, PhD, a Developmental Editor at F1000Research ...

...activity back to polymerase. In our case, dGTP will be used in the reaction, meaning that T4 Pol will remove...appropriate). The primer length is dependent on the T4 Pol "chew back" reaction, in which a single dNTP is included...guanosine residue and become stalled. A typical T4 Pol reaction is shown. Mix the components (add the T4...at room temperature for 30 minutes. Inactivate T4 Pol by heating to 75° for 20 minutes. Volume (μl) Reagent...will interfere with the exonuclease activity of T4 Pol in the following step. This may be accomplished by...Overhangs Treat your purified PCR product with T4 Pol in the presence of dCTP (not dGTP), corresponding...ligation. Because of its dual polymerase/exonuclease functions, T4 DNA polymerase can create overhangs of varying...

...resistant DNA polymerase from the thermophilic bacterium, Thermus aquaticus (Taq) . Taq polymerase can withstand...denature DNA polymerases from other species. In addition to the template DNA and the Taq polymerase, PCR requires... Taq polymerase has an optimal temperature around 70-75°C so this step enables the DNA polymerase to synthesize...elongation and synthesis. Taq DNA polymerase: Special DNA polymerase that can withstand radical temperature...Description of Polymerase Chain Reaction with protocol, tips and FAQ Protocols... Protocols Polymerase Chain Reaction...Reaction (PCR) Polymerase Chain Reaction (PCR) You may also like... Plasmid Cloning by PCR Restriction Digest...

...incorporated into HIV-1 particles independently of the Gag/Pol precursor protein. The EMBO Journal, 16(16), 5113...viral vector particles are made while missing the polymerase gene may seem like a mystery. But it’s simple... M., & Bishop, J. M. (1973). RNA-directed DNA polymerase of Rous sarcoma virus: Initiation of synthesis...

...scaffold) in a pol-III (OsU3) promoter driven expression system. We use a single polycistronic tRNA-gRNA (...inosine), which is treated as ‘G’ by cellular polymerases. Subsequently, an original genomic A-T base pair...

...genes (viral Gag-Pol): Gag is a structural precursor protein, and Pol is a polymerase. Envelope gene (...the same gene products for packaging (i.e., Gag, Pol, and Env), the isoforms of these proteins, as well... a packaging cell line that stably expresses Gag-Pol and/or Env, eliminating the need to deliver these...developed by Garry Nolan at Stanford contains Gag-Pol and either an ecotropic envelope, Phoenix-ECO, (for.... Another variant, Phoenix-gp, contains only Gag-Pol and enables additional flexibility in pseudotyping...lentiviruses and standard γ-retroviruses use the Gag, Pol, and Env genes for packaging; however, they are different...containing Matrix, Capsid, and Nucleocapsid components. Pol in trans Precursor protein containing Reverse Transcriptase...

...All on one plasmid: Gag, Pol, Rev, Tat Two plasmids: one encoding Gag and Pol and another encoding Rev... to drive RNA pol III-directed transcription of shRNAs. cDNA expression requires RNA pol II, and thus ...contains a single packaging plasmid encoding the Gag, Pol, Rev, and Tat genes. The transfer plasmid contains...plasmids: one encoding Rev and one encoding Gag and Pol. Although safer, this system can be more cumbersome...generation packaging systems: express the HIV gag, pol, rev, and tat genes all from a single packaging plasmid...3rd generation packaging systems: express gag and pol from one packaging plasmid and rev from another, ...lentiviruses and standard retroviruses use the gag, pol, and env genes for packaging; however, they are different...

...pOpen-T4gene43 T4 DNA Polymerase 165532 pOpen-ECOpolA E. coli DNA Polymerase I 165533 pOpen-TthpolA Tth DNA polymerase... T7 DNA Polymerase (unmodified) 165542 pOpen-KTHEAQpol KlenTaq1 polymerase 165543 pOpen-Bstpol Bst DNA...Fragment 165562 pOpen-Pwopol Pwo DNA Polymerase 165563 pOpen-BsupolLF Bsu DNA Polymerase I, Large Fragment... DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase 165501...165501 pOpen-GBDpol Pyrococcus sp. Heat-Stable DNA Polymerase 165502 pOpen-9N7polA (CT) 9 degrees N-7 DNA...DNA Polymerase chain terminating 165511 pOpen-K12polLF DNA Polymerase I, Large (Klenow) Fragment 165522...pOpen-K12polLF (exo-) DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-) 165512 pOpen-THEAQpolA Taq ...

...His, TEV polH Parkinson's Michael J Fox Foundation MJFF 40375 pBACgus/LRRK2 C-His LRRK2 His polH Parkinson's...His polH Parkinson's Michael J Fox Foundation MJFF 40379 pBACgus/LRRK2 C-His_T2031E LRRK2 His polH Parkinson's...His polH Parkinson's Michael J Fox Foundation MJFF 40381 pBACgus/LRRK2 C-His_G2019S LRRK2 His polH Parkinson's... LRRK2 His polH Parkinson's Michael J Fox Foundation MJFF 40385 pBACgus4x/Parkin PRKN polH Parkinson's...GST polH Parkinson's Michael J Fox Foundation MJFF 40407 LRRK2_1867_2143 LRRK2 His, TEV, GST polH Parkinson's...GST polH Parkinson's Michael J Fox Foundation MJFF 40409 LRRK2_1867_2134 LRRK2 His, TEV, GST polH Parkinson's... polH Parkinson's Michael J Fox Foundation MJFF 40505 pEMB018 / LRRK2_BC_1309_2187 LRRK2 His polH Parkinson's...

...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase, catalytic, gamma polypeptide PI3CG, PI3K...gonadotropin, beta polypeptide CGB3, hCGB CGB1 chorionic gonadotropin, beta polypeptide 1 - CGB2 chorionic... gonadotropin, beta polypeptide 2 - CGB5 chorionic gonadotropin, beta polypeptide 5 HCG, MGC119822 CGB7... beta polypeptide 7 CG-beta-a, FLJ35403, FLJ43118 CGB8 chorionic gonadotropin, beta polypeptide 8 - CHGA... - GIP gastric inhibitory polypeptide - GIPR gastric inhibitory polypeptide receptor MGC126722 GKN1 gastrokine...factor alpha polypeptide PDGF-A, PDGF1 PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma...

...Single polycistronic lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral...27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression of human...(8):1221-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for...Non-integrating mini-intronic plasmid (MIP) polycistronic expression of human Oct4, Klf4, Sox2, c-Myc ...episome Human Non-integrating EBNA1-mediated polycistronic expression of human Sox2, KLF4, L-Myc, Lin28...Replicating EBNA1 episome Human Non-integrating polycistronic expression of human Oct4, Klf4, Sox2, c-Myc,...episome Human Non-integrating EBNA1-mediated polycistronic expression of human Oct4, Sox2, Myc, Klf4, BCL2L1...

...lower background signals than polyclonal antibodies. However, polyclonal antibodies are more sensitive...clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that are specific... antibodies are typically more expensive than polyclonal antibodies. Environmental factors, binding partners...available epitope on your protein of interest. Polyclonal antibodies are typically collected directly from...targeting various epitopes on the same antigen. Polyclonal antibodies are often more environmentally stable...protein in any form it may be present in. However, polyclonals can vary significantly from lot to lot, as immune...consider the following questions: Do you need polyclonal or monoclonal antibodies? Will you be using a...

...dependent 1, 5 Yizhar 154948 CaMKII-somBiPOLES-mCerulean CamKII BiPOLES (soma-targeted) mCerulean Constitutive... 5, 9, rg* Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn BiPOLES (soma-targeted) mCerulean Cre dependent... Jaws stGtACR iC++ OPN3 PdCO PPO Bidirectional BiPOLES Promoter CAG CaMKII CBA Dlx EF1a/nEF Synapsin E2...

... PCR and surveyor. I used DNA polymerase Takara Ex Taq ™ DNA Polymerase for my genomic PCR, but couldn't...In our hands, Herculase II Fusion polymerase or Kapa Hifi Polymerase work very well. Some groups have ...your HR by doing Restriction Fragment Length Polymorphism (RFLP) ( see Figure 4 of the Cong, et. al, 2013...

...Marchantia polymorpha MpU6 none S. pyogenes Hara-Nishimura pMpGE_En03 71535 Other/Marchantia polymorpha MpU6...pyogenes Ventura pCRISPRyl 70007 Other/Yarrowia lipolytica SCR1'-tRNA yes, cut S. pyogenes LEU2 Wheeldon...pyogenes Draetta pCAS1yl 73226 Other/Yarrowia lipolytica yes, cut S. pyogenes LEU2 Yang pLCKO 73311 Mammalian...Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with up to 3 ...in a single vector. sgRNAs are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG ...

...Ligation Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated...acceptable. The bulk sorted cells are composed of a polyclonal population exposed to sgRNA-A and sgRNA-B (see... band” in separate reactions. NOTE: Numerous polymerases may be used for step 6.3 . Run samples in a thermocycler...a single reaction. Optimize multiplexing in a polyclonal population ( i.e., bulk sorted cells) before ...biallelic deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning...

...mEGFP EZH2 Polycomb repressive complex 2 164500 POLR2A-mEGFP AICSDP-117 mEGFP RPB1 RNA polymerase II 168798...

...Mammalian TAA Simon Elsaesser 157925 pMAH-POLY-eRF1(E55D) POLY-eRF1(E55D) E. coli para-substituted phenylalanine...proteins for various purposes from therapeutics to biopolymers. Tips for Success Before beginning to reprogram...azidonorleucine (Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG...