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We narrowed to 532 results for: SHI

Showing: 21 - 40 of 532 results
  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...entry with other transfection methods. The Junwei Shi Lab addressed this problem by optimizing the Cas ...Monomeric StayGold variants By Mike Lacy In 2022, Atsushi Miyawaki and colleagues identified a highly bright...
  2. CRISPR Guide

    Type
    Guide
    ..., 156 (5), 935–949. PMID: 24529477 Nishimasu, H., Shi, X., Ishiguro, S., Gao, L., Hirano, S., Okazaki,...Wang, H., Yang, H., Shi, L., Katz, Y., Theunissen, T. W., Rangarajan, S., Shivalila, C. S., Dadon, D. B...30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert...Reviews Genetics . 16 (5), 299–311. PMID: 25854182 Shi, J., Wang, E., Milazzo, J. P., Wang, Z., Kinney, ... Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N.,...32203383 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., ... Cell , 46 (5), 595–605. PMID: 22521689 Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and...
  3. Lentiviral Vector Guide

    Type
    Guide
    ...23311447 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert...10.1126/science.1247005 PMID: 24336571 Shao, L., Shi, R., Zhao, Y., Liu, H., Lu, A., Ma, J., Cai, Y., ...
  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, ...plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi, H.A., Funabiki, H. (2024) MagIC-Cryo-EM: Structural...
  5. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    .../genetics.115.176594 Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R (2013) One-Step Generation... find that many, if not all, the mice carry a frameshift mutation. For knock in with a donor oligonucleotide... genome. However, with only 3.4% of founders establishing germline transmission of the transgene, this...https://doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD...doi.org/10.1038/s41467-017-02706-7 Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R ...
  6. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...determine the best concentration for your assay. Washing steps can be done by hand but if available, use...pipette to add 200 µL wash buffer to each well. Washing can be done by hand but if available, use an automated...
  7. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ...comfortable shoes that cushion your feet. Eye protection such as goggles and/or face shields can be used as needed...Close-toed shoes Gloves Eye protection and face shields, as needed Guidelines Your lab coat should be buttoned...
  8. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ... 2011. 767: p. 411-8. PubMed PMID: 21822892. 33. Shi, Y., P. Kirwan, and F.J. Livesey, Directed differentiation...thanks to our guest blogger, Kusumika (Kushi) Mukherjee. Kusumika (Kushi) Mukherjee is the Editor of Trends...This post was contributed by Kusumika (Kushi) Mukherjee. The ultimate goal in the field of regenerative... pursue a career in science communication and publishing after completing her postdoctoral training from...441(7097): p. 1061-7. PubMed PMID: 16810240. 2. Takahashi, K., et al., Induction of pluripotent stem cells... 131(5): p. 861-72. PubMed PMID: 18035408. 3. Takahashi, K. and S. Yamanaka, Induction of pluripotent ...
  9. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ....2013.04.025  Yang, H., Wang, H., Shivalila, C. S., Cheng, A. W., Shi, L., & Jaenisch, R. (2013). One-Step...10.1016/j.cell.2011.06.019  Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F...
  10. Optogenetics AAV Preps

    Type
    Collection
    ....1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Karl Deisseroth 130995...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Karl Deisseroth 130998...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent 1, 5 Karl Deisseroth 131004...6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive 8 Karl Deisseroth 137158 ...pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Karl Deisseroth 137159...Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Cre and Flp dependent 8 Karl Deisseroth...Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Flp dependent 8 Karl Deisseroth 137161...
  11. Antibody Production

    Type
    Collection
    ... testing, it may ship at room temperature. However, Addgene determines ideal shipping conditions for each...order, ensuring each item ships safely and most economically. Actual shipping conditions will be shown ...(Link opens in a new window) page. Storage and Shipping Stability Addgene does not recommend repeated ...20 °C. Each antibody catalog item is tested for shipping stability by incubating at 37 °C for 2 weeks, ...
  12. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mStayGold Atsushi Miyawaki 212019 pcDNA3/F-tractin=mStayGold Actin filaments F-tractin mStayGold Atsushi Miyawaki...EGFP Noboru Mizushima 38272 pMXs-IP GFP-WIPI-1 Autophagosomes WIPI1 EGFP Noboru Mizushima 21074 ptfLC3...19031 pABCb10-GFP Mitochondria ABCb10 EGFP Orian Shirihai 27705 CAV1-mCherry Caveolae Cav1 mCherry Ari Helenius...Golgi apparatus in vesicles to be packaged and shipped. On the other hand, the endolysosomal pathway recylces...ptfLC3 Autophagosomes LC3 mRFP1, EGFP Tamotsu Yoshimori 110060 FUW mCherry-GFP-LC3 Autophagosomes LC3 mCherry...)=GianCreg Golgi apparatus Giantin td5StayGold Atsushi Miyawaki 231777 pCMV-mGold2t-MannII-N-10 Golgi ...
  13. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...material received. Shipping While Addgene assists importation by including thorough shipping documents and ...Packaged on Request material is generally ready for shipment within 8 weeks after the approval of the MTA by...may not yet have been completed at the time of shipment. If any discrepancies are identified during this...request, within three years of the order being shipped, Addgene can provide the QC validation data performed...
  14. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...plasmid page and will be shipped as bacterial stabs. Add to Cart $ 385 USD + shipping Available to academics...and 3 individually packaged strains, which are shipped as bacterial stabs at room temperature. Individual...19798082 . Kit Contents The following items will be shipped as stabs at room temperature. ID Name 13421 pAC-Kan-alphaGal4...
  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...genetically encoded calcium sensor T-GECI Blue-shifted genetically encoded Ca 2+ indicator with enhanced...protein, QUEEN-37C. bioRxiv 2021.10.08.463131. Yasushi Okada ATP (extracellular) GRAB_ATP1.0/ATP1.0mut...Biol. 2021 Jun 29. pii: S2451-9456(21)00301-9. Takashi Tsuboi Glucose iGlucoSnFR-TS biosensor for intracellular...cell imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Maltose Single fluorescent protein biosensor...cell imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Pyruvate Green (GreenPy1) and red (ApplePy1.... Mol Cell. 2016 Nov 17;64(4):835-849. Noboru Mizushima Autophagy Autophagosome maturation reporter (pmRFP-LC3...fluorescent-tagged LC3. Autophagy. 2007;3(5):452-60. Tamotsu Yoshimori Autophagy Expresses pHluorin-mKate2-hLC3 (PK-hLC3...
  16. AAV Packaged on Request

    Type
    Collection
    ...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...approval, your institution’s approval of the MTA, and shipping, mean that the actual time from request to delivery...
  17. Ligation Independent Cloning

    Type
    Protocol
    ...stop the exonuclease function of the enzyme and shift its activity back to polymerase. In our case, dGTP...
Showing: 21 - 40 of 532 results