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Showing: 21 - 40 of 649 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...based on shared characteristics and evolutionary similarity. These are grouped into two large classes based...conditions. Genome editing with CRISPR is not only simple, but also scalable. Multiple gRNAs targeting multiple...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...of genes and genetic elements. Here we present a simple methodology for CRISPR design, cloning, and delivery...plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but does not...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols...cancer modeling Using Cas9 mice, Platt et al . simultaneously modeled the dynamics of KRAS , p53 and LKB1...

  4. Bacterial Expression Systems

    Type
    Collection
    ...proper conditions and, epitope tags can be used to simplify the purification process. Using plasmids in this...arabinose binding protein (AraF) in the plasmid. Other similar reporters for detecting maltose can be found here...

  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ...with sequence similarity 3, member C GS3786, ILEI FAM3D family with sequence similarity 3, member D EF7...FLJ16023, KIAA0832, NR3B3 FAM3B family with sequence similarity 3, member B 2-21, C21orf11, C21orf76, ORF9, PANDER...platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) FLJ12858...leukocyte protein of 76kDa) SLP-76, SLP76 LOC652578 similar to Fc fragment of IgG, low affinity IIIa, receptor...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV ALS Sanford Simon 115331 pLNCX2-mCherry-CHMP2B CHMP2B mCherry CMV ALS Sanford Simon 115333 pLNCX2-mCherry-CHMP2B-siRNAres...Green 50487 pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ...-CHMP2B-siRNAres CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human CSF-1Rwt-IRES-GFP CSF1R Hereditary...pcDNA4/TO/GFP-GIGYF2 GIGYF2 GFP CMV Parkinson's Simon Bekker-Jensen 141323 pENTR-TARDBP-G287A TARDBP ALS...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Zap) New alternately colored FRET sensors for simultaneous monitoring of Zn(2)(+) in multiple cellular ...detect subthreshold membrane potential changes Simultaneous two-photon imaging of action potentials and ...

  8. Depositor Collections

    Type
    Collection
    ...mini-promoters to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant...

  10. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none...

  12. TALEN Engineering

    Type
    Collection
    ... transcriptional activator target sites and to simplify the construction of TALEN expression plasmids ...

  14. Antibody Plasmid Collection

    Type
    Collection
    ...recombinant antibody fragments in the form of scFvs A simple vector system to improve performance and utilisation...

  15. AAVED

    Type
    Collection
    ...experimental goals. How does this method compare to other similar methods? Where does this method fit in the big ...

  16. Plant Plasmids and Resources

    Type
    Collection
    ...system based on tobacco rattle virus (TRV) that simplifies gene silencing and genome editing logistics -...

  17. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... enhancer. pAdx-CMV-LacZ is uniquely suited to simplify the cloning process as it can be used in blue-...

  18. Zebrafish Plasmid Collection

    Type
    Collection
    ...cumulative genome editing - Jay Shendure Lab Simultaneous single-cell profiling of lineages and cell types...

  20. Ras Pathway

    Type
    Collection
    ...proto-oncogene Raf-1 proto-oncogene RAL RALA RALB v-ral simian leukemia viral oncogene homolog (ras related) RALBP1...
Showing: 21 - 40 of 649 results