We narrowed to 665 results for: SIM;

...gene, as well as a target site common to a set of similar sequences. Organisms include human, mouse, rat...for NmCas9 and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49...

...imaging of actin filaments with mGold2. Top: TIRF-SIM imaging of COS-7 cells expressing LifeAct fused to... (learn more in our recent blog post on TnpB). Similar to CRISPR-Cas, TnpB is directed to genomic targets...Rodriguez-Fraticelli recently developed an approach called Simultaneous Tracking of Recombinase Activation and Clonal...

...soma-targeted) mCerulean Constitutive 5, 9, rg* Simon Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn...soma-targeted) mCerulean Cre dependent 5, 9, rg* Simon Wiegert No available items found to match all requested...

...based on shared characteristics and evolutionary similarity. These are grouped into two large classes based...conditions. Genome editing with CRISPR is not only simple, but also scalable. Multiple gRNAs targeting multiple...

...-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols...cancer modeling Using Cas9 mice, Platt et al . simultaneously modeled the dynamics of KRAS , p53 and LKB1...

...non-specific labeling observed Publications Ben-Simon, Y., Hooper, M., Narayan, S., Daigle, T. L., Dwivedi...Bishaw, Y. M., Bendrick, J. L., Gore, B. B., Ben-Simon, Y., Opitz-Araya, X., Martinez, R. A., Way, S. W...

...with sequence similarity 3, member C GS3786, ILEI FAM3D family with sequence similarity 3, member D EF7...FLJ16023, KIAA0832, NR3B3 FAM3B family with sequence similarity 3, member B 2-21, C21orf11, C21orf76, ORF9, PANDER...platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) FLJ12858...leukocyte protein of 76kDa) SLP-76, SLP76 LOC652578 similar to Fc fragment of IgG, low affinity IIIa, receptor...

...Zap) New alternately colored FRET sensors for simultaneous monitoring of Zn 2+ in multiple cellular locations...detect subthreshold membrane potential changes Simultaneous two-photon imaging of action potentials and ...

...mini-promoters to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant...

...CMV ALS Sanford Simon 115331 pLNCX2-mCherry-CHMP2B CHMP2B mCherry CMV ALS Sanford Simon 115333 pLNCX2-mCherry-CHMP2B-siRNAres...Green 50487 pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ...-CHMP2B-siRNAres CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human CSF-1Rwt-IRES-GFP CSF1R Hereditary...pcDNA4/TO/GFP-GIGYF2 GIGYF2 GFP CMV Parkinson's Simon Bekker-Jensen 141323 pENTR-TARDBP-G287A TARDBP ALS...

...including that it is easy to implement due to the simplicity of gRNA design, programmable for different genomic...

...of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry to genes of...

... transcriptional activator target sites and to simplify the construction of TALEN expression plasmids ...

...expressing mCherry and firefly luciferase to simultaneously measure the # of cells containing reactivated...

... OPEN OPEN cCGCTTCAGCggtctGTGGACTCGa zebrafish similar to grp151 (or gpcr-2037) OZ535 and OZ536 OPEN OPEN...

...recombinant antibody fragments in the form of scFvs A simple vector system to improve performance and utilisation...

...TE) assays (Link opens in a new window) have a similar premise, but examine the proximity of a chemical...

...pAdx-CMV-LacZ (Addgene #73345) is uniquely suited to simplify the cloning process as it can be used in blue–...

...mNaChBac and (physically separate) tdTomato. 8 Massimo Scanziani 34910 paavCAG-pre-mGRASP-mCerulean CAG-driven...

...a "helper" virus, such as adenovirus or herpes simplex virus, to replicate in the host cell and complete...