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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 863 results for: SOM

Showing: 21 - 40 of 863 results

  1. TALEN Guide

    Type
    Collection
    ...that recognize various nucleotide triplets. With some trial and error, ZF arrays are able to recognize...average genome are every 500 bp. TAL effectors have some restrictions (for example, the target must start...Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. ...Pubmed PMID: 21493687 . Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander...

  2. Bacterial Expression Systems

    Type
    Collection
    ...that’s interesting from an applied perspective, sometimes it’s best to delete genes from or introduce new... expressed, you’ll likely need to tag it with something that makes it easy to see or easy to blot. The...this plasmid. Return to Top Protein Purification Sometimes looking at a protein inside cells just isn’t enough...detect the presence of that small molecule and sometimes even quantify it. Bacterial two-hybrid systems...

  3. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium ...CRISPR-dCas9 gene regulation has also been established for some of these parasites. Together, these tools have enabled...and development. This collection brings together some of the plasmids that have been used in these efforts...

  4. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ..., as titered by droplet digital PCR (ddPCR) . In some cases, we may expect lower titers for a particular...here or by email, Addgene will contact you to offer some options for moving forward with a helpful solution...

  5. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ..., SC22CB-5E3.5, NR1C1, PPAR, PPARalpha, hPPAR peroxisome proliferator-activated receptor alpha PPARD Plasmids... PPARD, FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB peroxisome proliferator-activated receptor delta PPARG Plasmids...CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma peroxisome proliferator-activated receptor gamma RARA Plasmids...

  6. Antibody Production

    Type
    Collection
    ...conditions will be shown in your cart. Need Some Help? Browse some of our other resources to help you choose...catalog page or by contacting [email protected] . Some of the antibodies distributed by Addgene were developed...

  7. Microbiology Resources

    Type
    Collection
    ...Leishmania sp. Plasmodium sp. Toxoplasma gondii Trypanosoma sp. Plasmids for Viruses Species Dengue Hepatitis... (WNV) Zika Don’t see your field listed? Know of some great plasmid tools for the community that aren'...plasmid kit : Integration of DNA into prokaryotic chromosomes - Endy and Shearwin Labs Ralstonia-GG-Kit : Assembly...

  8. Arf GTPase Family

    Type
    Collection
    ...traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid droplets, the nucleus...full list of plasmids available. Please note that some genes may not yet have plasmids available for request...

  9. Viral Vectors

    Type
    Collection
    ...genome, with some having an RNA genome while others have a DNA genome. In addition, some viruses function... of the strong immunogenic response they induce. Some (oncolytic) adenoviruses are utilized for cancer...

  10. Ras Pathway

    Type
    Collection
    ...RPS6KA2 RPS6KA3 RPS6KA6 Ribosomal protein S6 kinase RPS6KB RPS6KB1 RPS6KB2 Ribosomal protein S6 kinase B1...subunits - Class I PIN1 Peptidylprolyl cis/trans isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein...

  11. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... 2018). Iterative Synthetically Phosphorylated Isomers (iSPI). This new resource described in (Gassaway...188535 ). Importantly, phosphorylation positional isomers are allocated to different subpools, allowing precise...Protocols Iterative Synthetically Phosphorylated Isomers (iSPI) described in: A multi-purpose, regenerable...

  12. Lentivirus Plasmids

    Type
    Collection
    ...Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and useful types of viruses used...enabling long term transgene expression. There are some safety considerations to evaluate when working with...) are generally divided among multiple plasmids. Some plasmids (typically called packaging and envelope...

  13. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...3-4bp upstream of the PAM sequence. There can be some off-target DSBs using wildtype Cas9. The degree ...efficient guide (most guides would work, but they sometimes have different efficiency). And it is best if ...polymerase or Kapa Hifi Polymerase work very well. Some groups have successfully used Herc II or Kapa Hifi...

  14. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...transgenics, and more. See the next two tables for some other common uses. Viral Vector Delivery Although...Although transient expression is sufficient for some experiments, scientists often want to create stable...viral vector page for more information. Below are some common delivery methods. Delivery method...

  15. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... genes employ varied mechanisms to block CRISPR. Some prevent the CRISPR-Cas complex from binding to DNA...targeting : Cas13 enzymes target RNA rather than DNA, sometimes requiring a protospacer flanking sequence (PFS...Pederson T. 2015. Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...libraries in Addgene’s collection differ based on some important criteria, and it is important to know ... library best suits your particular experiment. Some important variables include: Type of genetic modification... 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout Human Sabatini...

  17. Antibodies

    Type
    Collection
    ...explain common applications. View Guide Need Some Help? Browse some of our other resources to help you choose...

  18. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Other/Trypanosoma cruzi yes, cut S. pyogenes Neo Docampo tdTomato/pTREX-b 68709 Other/Trypanosoma cruzi...without expression of Cas9. Highlighted below are some multiplex gRNA vectors from our collection. Plasmids...

  19. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...transposase components (TnsA, TnsB, TnsC, and TniQ). Some type I CASTs can result in bidirectional insertions...most often used in bacteria genome editing, though some mammalian systems have been developed. Figure 1:...

  20. All Antibodies

    Type
    Collection
    ...the National Institutes of Health. Need Some Help? Browse some of our other resources to help you choose...
Showing: 21 - 40 of 863 results