...assist with proper protein folding, and deal with sub-optimal codon usage (Table 1) A few mutations are...

...a Good Conflict Resolver? The first and foremost sub-skill to have here is pretty intuitive: You need ...also be categorized as either “personal” or “substantive”. A personality-based conflict occurs when two...a lab or work environment if left unchecked. Substantive conflict occurs when people or teams who normally...”) when in fact many sources of conflict are substantive (“Person C told Person A one thing and inadvertently...compromise and arbitration might remedy short-term substantive conflicts, but they could also aggravate long-term...

... biosensors can be imaged at spatial scales from sub-cellular to whole-body in small animals, such as ...intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps...antigen-dependent fluorescent nanobodies. Nat Methods submitted (2021). Tchekanda, E., Sivanesan, D. & Michnick...

...adding different ligands to the same sample to label sub-populations of your protein of interest. For example...range of useful molecules linked to that substrate. If the substrate was linked to, say, a chemical dye or...instead become trapped in a covalent bond with its substrate. Then, all it takes is a bit of chemistry magic... gets covalently linked to derivatives of its substrate O6-benzylguanine (BG) (Keppler et al., 2003). ...be labeled with fluorescent derivatives of its substrate trimpethoprim (TMP-tag) (Miller et al., 2005)...., or other uses of your protein, such as with substrate-linked PEG, biotin, or magnetic beads. In one ...

...of techniques have been developed that allow for sub-diffraction limit imaging. Most often, these techniques...number of optical and chemical ‘tricks’ to turn subsets of fluorescent molecules on and off. This can provide...-time monitoring of neuronal activity across substantial 3D volumes. In addition to 2-photon microscopy...

... the plant-specific parts you‘ll need, including sub-cellular localization signals, plant markers, and...

...transgenes via increased nuclear export. LTR Sub-components: U3 R Sub-element: TAR U5 in cis LTR; Long terminal... bigger than ∼3kb are packaged less efficiently. Sub-components: U3; Unique 3'; region at the 3' end of...retro/lentiviral vectors. Tat binds to this region. Sub-element: TAR; 2nd generation only; Trans-activating...lentivirus and a retrovirus? Lentiviruses are a subtype of retrovirus. From an experimental standpoint ...possible approaches to using this site include subcloning or appending compatible restriction sites onto... insert of interest using PCR. The process of subcloning consists of digesting the insert of interest ...

... NTCP2 TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) ABC17, ABCB2, APT1, D6S114E, FLJ26666... TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) ABC18, ABCB3, APT2, D6S217E, PSF2...receptor subfamily C, member 3 NKG2-E, NKG2E KLRC4 killer cell lectin-like receptor subfamily C, member...macropain) 26S subunit, ATPase, 3 MGC8487, TBP1 PSMC4 proteasome (prosome, macropain) 26S subunit, ATPase, ...) 26S subunit, non-ATPase, 10 dJ889N15.2, p28 PSMD11 proteasome (prosome, macropain) 26S subunit, non-... 26S subunit, non-ATPase, 14 PAD1, POH1, rpn11 PSMD2 proteasome (prosome, macropain) 26S subunit, non-...macropain) 26S subunit, non-ATPase, 3 P58, RPN3, S3 PSMD4 proteasome (prosome, macropain) 26S subunit, non-ATPase...

...resource subdivides the 110,139 human phosphoserine site library ( 188536 ) into 10 subpools of ~11,000...Pooled Library 188526 iSPI_pSer_Subpool#1 Pooled Library 188527 iSPI_pSer_Subpool#2 Pooled Library 188528...188528 iSPI_pSer_Subpool#3 Pooled Library 188529 iSPI_pSer_Subpool#4 Pooled Library 188530 iSPI_pSer_Subpool...Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library 188532 iSPI_pSer_Subpool#7 Pooled Library 188533...188533 iSPI_pSer_Subpool#8 Pooled Library 188534 iSPI_pSer_Subpool#9 Pooled Library 188535 iSPI_pSer_Subpool... can then be used to identify candidate human substrates of kinases of interest by mass spectrometry in... positional isomers are allocated to different subpools, allowing precise knowledge of phosphorylated ...

... DSS, GTD, HHG, NROB1, SRXY2 nuclear receptor subfamily 0, group B, member 1 NR1D1 Plasmids NR1D1, EAR1...EAR1, hRev, THRA1, THRAL, ear-1 nuclear receptor subfamily 1, group D, member 1 NR1D2 Plasmids NR1D2, RVR...RVR, BD73, EAR-1R nuclear receptor subfamily 1, group D, member 2 NR1H3 Plasmids NR1H3, LXR-a, LXRA, RLD...RLD-1 nuclear receptor subfamily 1, group H, member 3 NR2E1 Plasmids NR2E1, RP3-429G5.1, TLL, TLX, XTLL...XTLL nuclear receptor subfamily 2, group E, member 1 NR2F1 plasmids NR2F1, COUP-TFI, EAR-3, EAR3, ERBAL3...NR2F2, SVP44, TCFCOUP1, TFCOUP1 nuclear receptor subfamily 2, group F, member 1 NR3C1 Plasmids NR3C1, GCCR...GCCR, GCR, GR, GRL nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) NR3C2 Plasmids...

...Catalytic subunit alpha B1,B2: non-catalytic subunit beta G1, G2, G3: non-catalytic subunit gamma PTEN...protein 1 PIK3 Catalytic Subunits PIK3CA PIK3CB PIK3CD PIK3CG Regulatory Subunits PIK3R1 PIK3R2 PIK3R3 PIK3R4...the gene list below. For proteins with multiple subunits or isoforms, individual links to each gene page...plasmids for the gene. For components with multiple subunits or isoforms, individual links to each gene page...receptor-related receptor IRS IRS1 IRS2 Insulin receptor substrate JUN Jun proto-oncogene KSR KSR1 KSR2 Kinase suppressor...bisphosphate 3-kinase catalytic and regulatory subunits - Class I PIN1 Peptidylprolyl cis/trans isomerase...Plexin B1 PPP1CA Protein phosphatase 1 catalytic subunit alpha PREX2 Phosphatidylinositol-3,4,5-trisphosphate-dependent...

...Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service...blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA. Nat Commun. 2014 Jun ...genetically encoded sensors for population and subcellular imaging of cAMP in vivo. Nat Methods. 2022 Nov...FRET-based cGMP biosensor SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation. Cell Rep...2021 Jun 29. pii: S2451-9456(21)00301-9. Takashi Tsuboi Glucose iGlucoSnFR-TS biosensor for intracellular...imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Maltose Single fluorescent protein biosensor for...imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Sucrose FRET-based sensor to monitor sucrose levels...

...isozyme 1 PI3K Catalytic Subunits PIK3CA PIK3CB PIK3CD PIK3CG Regulatory Subunits PIK3R1 PIK3R2 PIK3R3 PIK3R4...and mTORC2. Each complex contains a unique subunit: raptor for mTORC1, and rictor for mTORC2. Background... the gene list below. For multiple isoforms or subunits, individual links to each gene page are provided...gene. For components with multiple isoforms or subunits, individual links to each gene page are provided...Neurofibromin 1 PRAS40 Also known as AKT1S1; AKT1 substrate 1 (proline rich) PTEN Phosphatase and tensin homolog...bisphosphate 3-kinase catalytic and regulatory subunits - Class I PKC PKC alpha PKC beta PKC delta PKC...

...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ensure...Isotype Description Configuration and Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears...should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate after thawing and therefore...reaction in the presence of several different substrates. The luminescence produced by the reaction can...different species of origin, isotypes, and isotype subclasses of a primary antibody, which becomes the limiting...and block the membrane. Activate the HRP with a substrate kit. Image the membrane using either X-ray film... cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires...

...-v2 subpooled libraries 83980 — 83986 Activation Human Weissman 3rd 5 Varies hCRISPRi-v2 subpooled libraries...-v2 subpooled libraries 83998 — 84004 Activation Mouse Weissman 3rd 5 Varies mCRISPRi-v2 subpooled libraries...the entire genome or a specific class of genes (subpools). Species - the gRNAs within a given CRISPR library...KSHV) Cow ( B. taurus ) Library Size Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ...Activation Human Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043...

...regions: Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum Whole...BGHpA AiP1277 AiE2051m SYFP2 Claustrum Cortical subplate 220711 pAAV-AiE2051m-minBG-iCre(R297T)-BGHpA AiP1786... AiP1786 AiE2051m Cre Claustrum Cortical subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m... AiE2051m FlpO Claustrum Cortical subplate 191720 pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA AiP13038 ...AiE0410m Cre Oligodendrocytes Whole Brain * = Substantial non-specific labeling observed Publications Ben-Simon...Tasic B. Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron. 2021 May 5;109(9):1449...JT, Levi BP. Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex...

...Bind to the alpha subunit, outside the ligand-binding pocket Bind to the beta subunit, outside the ligand-binding...pocket Bind specific subunit pairs, outside the ligand-binding pocket Bind specific subunit pairs, blocking...mechanotransduction receptors, comprised of alpha-beta subunit heterodimers. The IPI collection contains antibodies...

...Manipulating the genome directly can give you more subtle control over protein expression and activity thereby... and lacO variants that can be expressed in B. subtilis Various combinations of N and C-terminal Strep... express a protein of interest in E. coli , B. subtilis and P. pastoris . All plasmids use the same restriction...or their derivatives. Binding between the RNAP subunit α fusion protein in pBRα-β flap and the λCI fusion...pB1H2w derivatives as a fusion to RNA polymerase subunit ω. Transcription factor binding to a putative promoter...

...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...enhanced transduction of the retina following subretinal administration and enhanced transduction and ...transduction and lateral spread in the retina following subretinal injection. It was identified by John Chiorini...transduction and lateral spread in the retina following subretinal injection as compared to the parental AAV44.9...

...After production, all virus preps are titered and subjected to quality control by Addgene before being distributed...VP2 and VP3. Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed...Silver staining of purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins ...gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization purposes...