We narrowed to 954 results for: TES

...Phosphopeptide Library ). The phosphorylation sites (“phosphosites”) consist of a central phosphoserine site...of ~110,000 phosphosites is subdivided into 10 iSPI subpools of ~11,000 unique phosphosites. Iterative ... where it recognizes the UAG amber codon and incorporates phosphoserine into the growing polypeptide chain...phosphorylated proteins with one or more phosphorylation sites (Pirman et al., 2015). Improved phosphoprotein production...expressing recombinant phosphoproteins. These attributes make rEcoli XpS (Bacterial Strain #192872) and...site-specific incorporation of phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber et al., ...tRNA ( SupD (Plasmid #68307) ) to generate the phosphosites with serine instead of phosphoserine (i.e., ...

...additional sites throughout the genome. These sites are called off-targets. Be aware of off-target sites when...efficiency. So while testing multiple gRNAs is a good idea for most CRISPR experiments, testing multiple pegRNA...efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Scientific...that there could be downstream translation start sites that could allow for expression of a truncated protein...PAM sequences or Cas9 enzymes with flexible PAM sites . Base Editing : Target the site of your desired...complement to your target sequence with no homologous sites elsewhere in the genome. In reality, a given gRNA...genetic cargo (in this case, CRISPR components) integrates into the genome. Lentiviral vectors are a good...

...increasing on-target editing rates and decreasing off-target editing rates. Advances in this area include...offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful defensive...elucidated until 2007. Scientists hypothesized that prokaryotes used CRISPR as part of an adaptive immune system...would bind. (5) After the complex binds, Cas9 separates the double stranded DNA target and cleaves both...seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed repair (HDR) allows...packaged in AAV. Cas9 orthologs also have distinct PAM sites that do not cross react, allowing multiple Cas9s...engineered mutant Cas enzymes with alternative PAM sites . Cpf1 , another CRISPR nuclease, recognizes a 5...

...interesting adaptive immune response that detects and eliminates foreign DNA, which scientists have modified for... used for small precision edits. NHEJ directly ligates the break ends without the need for a homologous...large genomic deletions or inversions with two gRNA sites on the same chromosome. Most multiplex systems enable...most gRNA targeting sequences will have additional sites of partial homology throughout the genome, called...Modifications Using Homology Directed Repair (HDR) HDR generates specific genomic edits by copying from a DNA donor...mutation, while whole plasmid or linear dsDNA templates work better for large edits or insertions. When..., where Cas9n nicks the non-target strand and generates a 3’ flap. However, the longer pegRNA also encodes...

...a new window) . Figure 1: Selection, prep, and testing of AAV enhancers in mice. Enhancers were selected...different brain regions. Labeled cell populations were tested in mouse brains. This collection contains plasmids...minBG-SYFP2-WPRE3-BGHpA AiP12558 AiE0390m_3xC2 SYFP2 Astrocytes Whole Brain 208163 pAAV-AiE0390m_3xC2-minBG-iCre...-iCre(R297T)-BGHpA AiP14801 AiE0390m_3xC2 Cre Astrocytes Whole Brain 208143 pAAV-AiE0390h_3xC2-minBG-SYFP2...minBG-SYFP2-WPRE3-BGHpA AiP13572 AiE0390h_3xC2 SYFP2 Astrocytes Whole Brain 208133 pAAV-AiE0387m-minBglobin-SYFP2...-SYFP2-WPRE3-BGHpA AiP12102 AiE0387m SYFP2 Astrocytes Whole Brain 208166 pAAV-AiE0387m-minBG-iCre(R297T...R297T)-BGHpA AiP13999 AiE0387m Cre Astrocytes Whole Brain 220643 pAAV-AiE2128m-minBG-SYFP2-WPRE3-BGHpA AiP1357...

...response has developed, the antibodies are collected, tested against the antigen, packaged, and sold. This is...immortalized B cells). The genetic reorganization that creates variety in the antibody allowing for their extreme...class therefore has a valency, or number of binding sites available per antibody unit, which is typically ...multiplied by two, as each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses...Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving allergic reactions...in the lab. Check for and record any expiration dates. Buffers Buffers are a concern for conjugating and...reaction in the presence of several different substrates. The luminescence produced by the reaction can...

... mTORC1 integrates these signals to determine the nutritional state of the cell and regulates the switch...signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many cancers. Cancer...anabolic to catabolic processes. Active mTORC1 promotes protein and lipid synthesis, and increases energy...Enhanced signaling through either mTORC1 or mTORC2 correlates with poor prognosis in many cancers, including... cancer. Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial...mTORC1. The hyperactivation of mTORC1 in cancer promotes the conditions needed for uncontrolled cell growth... which appears to signal upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway ...

...ΔA all TAG sites changes to UAG, RF1 function removed George Church 48999 C321 all TAG sites changes to...all TAG sites changes to UAG, RF1 function removed Farren Isaacs 87359 C321.∆A.opt all TAG sites changes...to UAG George Church 49018 C321.ΔA.exp all TAG sites changes to UAG, RF1 function removed, MutS restored...mutation rate George Church 68306 C321.ΔA all TAG sites changes to UAG, RF1 function removed, deletion of...Strain 192872. Jesse Rinehart 69493 MCJ.559 TAG sites, RF1 function removed, genomic deletions for improved...incorporation Michael Jewett 69495 rEc.13.delA 13 amber sites changed to TAA, RF1 function removed Michael Jewett.... George Church 98564 C321.Ub-UAG-sfGFP all TAG sites changes to UAG, RF1 function removed, with Ubiquitin-UAG-sfGFP...

...functionally distinct interneurons in mice, non-human primates and humans. Nat Neurosci. 2020 Dec;23(12):1629...ER-targeted CEPIA1er ER-mitochondria tethering by PDZD8 regulates Ca 2+ dynamics in mammalian neurons. Science. ...Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Curr Biol...the adenosine sensor GRAB_Ado1.0 in neurons or astrocytes (Constitutive or Cre-dependent) Li Lab GRAB_Ado...cytoplasmic, membrane-displayed, expression in neurons, astrocytes) A genetically encoded single-wavelength sensor..., photostability and reduced ph interference demonstrates augmented transmembrane chloride movement by...biosensor for extracellular L-lactate, in neurons, astrocytes or mammalian cells A genetically encoded fluorescent...

... in mammalian systems. Parasites CRISPR plasmids designed for use in parasites. Plant CRISPR plasmids ...bacteria, yeast, Drosophila, zebrafish, C. elegans, parasites, and Xenopus. Addgene CRISPR Resources CRISPR ...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on ... promoting DSB formation. Delete Cascade-Cas3 generates large genomic deletions. Insert CRISPR transposases... Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...

...function of zebrafish astrocytes. Knock-in tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent...Zebrafish Society (IZFS) - An organization that promotes and advocates for zebrafish research in the international...Morsch Lab. A vertebrate model to study ALS-linked aggregates in real time. Live imaging of astroctyes - Kelly...Resources Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome...

...Institute Identifying Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine... Cancer Institute Finding the Causes of Small Intestinal Neuroendocrine Tumors Ramesh Shivdasani Dana-...Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou...Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley ...

...access tool from Synthego allows you to determine rates of CRISPR editing at a given locus. To use the tool... window) A tool that identifies putative target sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus...probability of off-target effects. Cas-Designer integrates information from Cas-OFFinder and Microhomology...a new window) Identifies candidate sgRNA target sites by off-target quality. Validated for gene inactivation...in a new window) Identification of sgRNA target sites and off-target evaluation for base edits using adenine...window) Can be used to identify novel gRNA target sites in a single gene, as well as a target site common...

...simultaneously with dual labeling when used with two substrates Can be used in live or fixed cells, and for internal...protein tags based on the acyl carrier protein. Substrates are derivatives of Coenzyme A (CoA). These tags... Please see the note below for information on substrates and synthases needed for use of these tags. Plasmids...product portfolio, including the CoA-derivative substrates and the ACP and SFP Synthases. Users may still... their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme A disodium salt from a...

...being used for their distinct features. Addgene distributes many plasmids that can help researchers plan ...western blots, or removed using protease cleavage sites. Other protein tags and signal peptides are used... reduces bacterial protein contamination and facilitates extraction. Browse our most popular plasmids ...protein purification. Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis...Solubility tags: MBP, SUMO, TrxA, Mocr, NusA Cleavage sites: TEV protease, factor Xa, enterokinase, thrombin...fluorophore if they are within a certain distance. You can test if two proteins bind or interact together by fusing...

...nuclease operates as a dimer, cutting in the spacer region between two distinct ZF target sites. (B) A ...species almost as easily as correcting a typo in the latest draft of your dissertation. Well, that will probably...corresponding zinc fingers, potential targetable sites in your average genome are every 500 bp. TAL effectors... a T), but they still have potential targetable sites approximately every 35bp. Researchers are still ...

...sequences and gRNA target sites. The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR ...plasmids in this CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human...plasmids in the following articles provide PCR templates for amplification of the tag (eg GFP, Flag, YFP...elegans . This system uses a 10-day protocol, generates “clean” homozygous mutants with no co-integrated...

...Cell type-biased: Astrocytes - AAV-PHP.AX with GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with...digital PCR, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated virus...regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER Center...

... TALE transcriptional activator target sites and then generates individual user-friendly graphical roadmaps... ( Sander et al., Nat Biotechnol. 2011 ) Rats ( Tesson et al., Nat Biotechnol. 2011 ) Human somatic cells...monomeric TALE transcriptional activator target sites and to simplify the construction of TALEN expression...

...inducible - VIP vasoactive intestinal peptide MGC13587, PHM27 VIPR1 vasoactive intestinal peptide receptor 1 ...immune response is triggered within the first few minutes of exposure to a pathogen and is the dominant defense...Chemokines recruit innate and adaptive immune cells to sites of inflammation throughout the body. Cytokines Cytokines...its cognate antigen along with co-stimulation activates the T cell, whereas T cell tolerance or anergy... NK cells, macrophages, dendritic cells, and granulocytes. TLR The toll like receptors (TLR) play a key...Chemokines recruit innate and adaptive immune cells to sites of inflammation throughout the body. Symbol Name...chemokine (C-C motif) ligand 5 D17S136E, MGC17164, RANTES, SCYA5, SISd, TCP228 CCL7 chemokine (C-C motif)...