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  1. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids...shuttle plasmids to insert a second guide within a U6-guide cassette. This process is compatible with all...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Yildiz 159787 pGL3-U6-pegRNA_KCNA1-EGFP KCNA1 U6 Episodic ataxia Xiaolong Wang 159788 pGL3-U6-sgRNA_KCNA1-mcherry...190900 pAAV2ss-U6-sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-...7sk-Cas9 HTT U6, 7sk Huntington's Nicole Deglon 190902 pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1 HTT GFP U6, EFS Huntington's...Deglon 190903 pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9 HTT GFP U6, EFS Huntington's Nicole Deglon 191487...78622 pX335_HR_Prnp_3 PRNP U6 Dementia Walker Jackson 78623 pX459_HR_Prnp_3 PRNP U6 Dementia Walker Jackson...MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP U6 Dementia Walker Jackson...SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS Rajat Rohatgi...

  3. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...none, need Cas9 plasmid Bullock and Port 49411 pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3 BbsI Injection ...Virmilion none, need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 ...

  6. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...47549 pDD162 (Peft-3::Cas9 + Empty sgRNA) R07E5.16 U6 yes, cut Goldstein 42250 DR274 T7 BsaI In vitro transcription...

  7. Retrovirus Plasmids

    Type
    Collection
    ...and Neo resistance Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses...

  8. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...target sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary to ...nucleotide to the start of my target sequence? The human U6 promoter prefers a 'G' at the transcription start...

  9. Cre-lox system

    Type
    Collection
    ... Nanog locus mNanog Mammalian Hanna 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Cre... and sgRNA expression EFS AAV Zhang 60229 AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR Cre and sgRNA... sgRNA coexpression Cbh AAV Zhang 60231 AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-...lentiviral vector Cbh Lentiviral Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre Cre Chicken cardiac troponin T AAV Pu...

  10. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...shRNA against mouse TNF-alpha mRNA from the mouse U6 promoter pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro 73022 ...

  11. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...of the 20-mer is not G. sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion... for each sample and sequence each colony using a U6 promoter forward primer: CGTAACTTGAAAGTATTTCGATTTCTTGGC...

  12. Mammalian RNAi Tools

    Type
    Collection
    ...Sabatini 11795 pLL3.7 Expresses shRNA under the mouse U6 promoter; A CMV-EGFP reporter cassette is included...

  13. Sequencing Primers

    Type
    Guide
    ...LKO.1 5' GACTATCATATGCTTACCGT (Weinberg Lab) Human U6 promoter, forward primer LucNrev CCTTATGCAGTTGCTCTCC...LKO.1 5' GACTATCATATGCTTACCGT (Weinberg Lab) Human U6 promoter, forward primer LNCX AGCTCGTTTAGTGAACCGTCAGATC... forward primer mU6-F CAGCACAAAAGGAAACTCACC Mouse U6 promoter, forward primer Myc GCATCAATGCAGAAGCTGATCTCA... TRC-F CAAGGCTGTTAGAGAGATAATTGGA (Root lab) Human U6 promoter, forward primer Ubx-F AACTCGTACTTTGAACAGGC...

  15. Promoters

    Type
    Guide
    ...Tetracycline response element promoter U6 Constitutive Human U6 nuclear promoter for small RNA expression...

  16. Plan Your Experiment

    Type
    Collection
    ...constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically used for gRNA May contain reporter...

  17. Lentiviral Guide

    Type
    Guide
    ...included within a transfer plasmid: for example, the U6 promoter is included in the pSico plasmid to drive...shRNA‐expressing lentiviral plasmids, such as pLKO.1, use a U6 or H1 promoter in order to drive RNA pol III-directed...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...

  19. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...

  20. Plan Your Experiment

    Type
    Guide
    ...constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically used for gRNA May contain reporter...
Showing: 21 - 40 of 40 results