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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 333 results for: cki

Showing: 21 - 40 of 333 results

  1. CRISPR Plasmids - Yeast

    Type
    Collection
    ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  3. AAV Packaged on Request

    Type
    Collection
    ...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...

  4. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  5. Arf GTPase Family

    Type
    Collection
    ... the archaeal origins of Eukaryotic Membrane-Trafficking System building blocks. Molecular Biology and...

  7. CRISPR Plasmids - Plants

    Type
    Collection
    ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  8. Open Enzyme Collection

    Type
    Collection
    ...Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) 165566 pOpen-TthRT Thermus thermophilus...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...it has been diluted. Mix by swirling or gently flicking the tube. Incubate for 5 minutes at room temperature...the walls of the tube. Mix by swirling or gently flicking the tube. f. Incubate for 20-30 minutes at room...

  10. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...business of iPSC clone picking, a 100-fold decrease is the difference between picking 100 versus 10,000 clones...efficiency.   But what’s better: PAM-blocking or guide RNA blocking mutations? This depends entirely on...efficiency of guide RNA blocking mutations. So if the locus does not allow blocking the PAM sequence (e.g...efficiency provided by blocking mutations. Essentially, you:  Step 1 - Introduce the blocking mutation together... incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our intended... same CRISPR/Cas-blocking mutation but not the intended mutation. Here, the blocking-only templates will...mutations. 1. Increasing HDR accuracy with CRISPR/Cas-blocking mutations After genome editing with CRISPR/Cas9...

  11. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...insufficient blocking) or reduced signal (too much blocking.) If you’re having issues with your blocking, first..., degraded or improperly mixed blocking buffer, or insufficient rocking during incubation steps.  Many...ResearchGate’s western blot forum.  Blocking Don’t skimp on the blocking time! Fill the time with smaller...  Troubleshooting Blocking reduces the amount of background in your blot, so blocking issues can either...you can try either longer blocking times or higher concentrations of blocking buffer. If you see a low ...Inefficient blocking Check antibody spec sheet for buffer recommendations Use a chemical blocking buffer...each lane is its own piece (3). After incubation, blocking, and washing, the membrane is reassembled and ...

  12. COVID-19 Resources

    Type
    Collection
    ... Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad Institute...

  13. Optogenetics AAV Preps

    Type
    Collection
    ...tdTomato Cre dependent 1, 5, 8 Boyden 105669 pAAV-CKIIa-stGtACR2-FusionRed CaMKII GtACR2 (soma-targeted)...

  14. Retrograde AAV viral preps

    Type
    Collection
    ...CamKII Inhibitor Optogenetics Boyden 105669 pAAV-CKIIa-stGtACR2-FusionRed CamKII Inhibitor Optogenetics...

  15. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...expression. Transgenic and knockin mice As the name suggests, transgenic and knockin mice contain an additional...Conditional knockouts or knockins A subset of transgenic mice, conditional knockout/knockin mice, use the Cre-lox...construct incorporated randomly into the genome, knockin mice have the gene construct inserted at a specific...DNA randomly into the mouse genome. In contrast, knockin strains are generated via homologous recombination...breaks, such as ZFN and TALEN, can used to engineer knockin mice. Co-injection of a targeting vector containing...based systems have been developed for generating knockin mice but because CRISPR/Cas9 engineering favors...following Cre-mediated recombination. In conditional knockin mice, the target gene is initially inhibited by...

  16. The Pleiades Promoter Project

    Type
    Collection
    ...Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK, Glenn R, ...

  17. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...cutters. You can also sort enzymes by clicking on column heads. Clicking on a particular restriction enzyme...GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software and extensive...Maps This is the first tab you’ll land on after clicking the “Analyze Sequence” button. The maps hosted...similar hover over features. As with the map tab, clicking on two separate locations in the linear sequence...Types listed are standard GenBank feature types. Clicking on a particular feature here also highlights that...binding site locations, lengths, and direction. Clicking on a particular primer here also highlights it...updates in the comments section below and keep checking in at blog.addgene.org for additional enhancements...

  18. Design Tips for Prime Editing

    Type
    Blog Post
    ...tool of choice. Nicking sgRNAs PE3 and PE5: These tools use an sgRNA to guide nicking of the unedited...editors bound to sgRNAs only drive nicking (right). Start with nicking sgRNAs ~50 bp from the prime editing... Figure 7: Nicking sgRNAs can be designed to target edited DNA, so that nicking only occurs after... prevents the Cas9 nickase from re-binding and nicking the newly synthesized strand before heteroduplex...BioRender.com. PE3b and PE5b: In this approach, the nicking sgRNA is designed to bind only after the edit is...
Showing: 21 - 40 of 333 results