Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 333 results for: cki

Showing: 21 - 40 of 333 results

  1. CRISPR Plasmids - Yeast

    Type
    Collection
    ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  3. AAV Packaged on Request

    Type
    Collection
    ...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...

  4. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  5. Arf GTPase Family

    Type
    Collection
    ... the archaeal origins of Eukaryotic Membrane-Trafficking System building blocks. Molecular Biology and...

  7. CRISPR Plasmids - Plants

    Type
    Collection
    ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  8. Open Enzyme Collection

    Type
    Collection
    ...Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) 165566 pOpen-TthRT Thermus thermophilus...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...it has been diluted. Mix by swirling or gently flicking the tube. Incubate for 5 minutes at room temperature...the walls of the tube. Mix by swirling or gently flicking the tube. f. Incubate for 20-30 minutes at room...

  10. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...business of iPSC clone picking, a 100-fold decrease is the difference between picking 100 versus 10,000 clones...efficiency.   But what’s better: PAM-blocking or guide RNA blocking mutations? This depends entirely on...efficiency of guide RNA blocking mutations. So if the locus does not allow blocking the PAM sequence (e.g...efficiency provided by blocking mutations. Essentially, you:  Step 1 - Introduce the blocking mutation together... incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our intended... same CRISPR/Cas-blocking mutation but not the intended mutation. Here, the blocking-only templates will...mutations. 1. Increasing HDR accuracy with CRISPR/Cas-blocking mutations After genome editing with CRISPR/Cas9...

  11. COVID-19 Resources

    Type
    Collection
    ... Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad Institute...

  12. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...insufficient blocking) or reduced signal (too much blocking.) If you’re having issues with your blocking, first..., degraded or improperly mixed blocking buffer, or insufficient rocking during incubation steps.  Many...ResearchGate’s western blot forum.  Blocking Don’t skimp on the blocking time! Fill the time with smaller...  Troubleshooting Blocking reduces the amount of background in your blot, so blocking issues can either...you can try either longer blocking times or higher concentrations of blocking buffer. If you see a low ...Inefficient blocking Check antibody spec sheet for buffer recommendations Use a chemical blocking buffer...each lane is its own piece (3). After incubation, blocking, and washing, the membrane is reassembled and ...

  13. Optogenetics AAV Preps

    Type
    Collection
    ...tdTomato Cre dependent 1, 5, 8 Boyden 105669 pAAV-CKIIa-stGtACR2-FusionRed CaMKII GtACR2 (soma-targeted)...

  14. Plan Your Experiment

    Type
    Collection
    ... the observed efficiency of CRISPR knockout or knockin. Select gene and genetic element to be manipulated...

  15. Retrograde AAV viral preps

    Type
    Collection
    ...CamKII Inhibitor Optogenetics Boyden 105669 pAAV-CKIIa-stGtACR2-FusionRed CamKII Inhibitor Optogenetics...

  16. The Pleiades Promoter Project

    Type
    Collection
    ...Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK, Glenn R, ...

  19. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...expression. Transgenic and knockin mice As the name suggests, transgenic and knockin mice contain an additional...Conditional knockouts or knockins A subset of transgenic mice, conditional knockout/knockin mice, use the Cre-lox...construct incorporated randomly into the genome, knockin mice have the gene construct inserted at a specific...DNA randomly into the mouse genome. In contrast, knockin strains are generated via homologous recombination...breaks, such as ZFN and TALEN, can used to engineer knockin mice. Co-injection of a targeting vector containing...based systems have been developed for generating knockin mice but because CRISPR/Cas9 engineering favors...following Cre-mediated recombination. In conditional knockin mice, the target gene is initially inhibited by...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...normal conditions, the TetR protein binds to tet O, blocking transcription of the downstream gene. If tet or...
Showing: 21 - 40 of 333 results