We narrowed to 633 results for: gats

...SpCas9. xCas9 has a PAM requirement of NG, GAA, or GAT, making it a more flexible cutter with the added ...

...-NGG PAM sequences include: xCas9 — NG, GAA, and GAT; increased nuclease fidelity SpCas9-NG — NG; increased...VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9... used for small precision edits. NHEJ directly ligates the break ends without the need for a homologous...system, CRISPRi is introduced into bacteria using conjugation and stably integrated into the chromosome. Browse...CJ) 3' NNNNRYAC Neisseria meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema...). Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9. Nature Communications , 15 (1)...on the consecutive binding and degradation of negatively supercoiled invader DNA by cascade and CAS3. ...

... Protocols Centrifugation Centrifugation Protocol Intro to the Lab Bench Check...centrifuge. Immediately stop the centrifuge and investigate the issue. When you are finished spinning your...

... Protocols Ligation Independent Cloning Ligation Independent ...Overview Ligation Independent Cloning (LIC) obviates the need for the time-consuming ligation step of ...Protocol for Ligation Independent Cloning Protocols...fragments, allowing for transformation without ligation. Because of its dual polymerase/exonuclease functions...mixture, and may provide counter-selection for negative clones as in the example shown here. The manufacturer...

...Iodixanol Gradient Ultracentrifugation AAV Purification by Iodixanol Gradient Ultracentrifugation You may also...Follow this protocol using Iodixanol gradient ultracentrifugation to get pure adeno-associated virus (AAV) ...Data Figure 1: Iodixanol gradient before ultracentrifugation. The steps are clearly distinguishable thanks... 2: Left panel: Iodixanol gradient after ultracentrifugation. The arrow indicates the 60–40% interface...

... For trickier ligations (such as ligation of annealed oligos) the efficiency of ligation can be improved... Protocols DNA Ligation DNA Ligation You may also like... Plasmid...transformed into bacterial cells for propagation. The majority of ligation reactions involve DNA fragments ...ultimately are fused by the ligation reaction. The example below depicts the ligation of two sticky ends that...orientation and prevents the vector from ligating to itself during the ligation process. If the sticky ends on ...: Standard Insert + Vector DNA Ligation Before setting up the ligation reaction itself, it is important...use for the ligation reaction. The volume of vector DNA and insert DNA used in the ligation will vary depending...

...components can interfere with the conjugate or conjugation process. Sodium azide, an antimicrobial agent,... back to buffers after the conjugation reaction is complete. HRP-conjugated antibodies should not be stored...done through a conjugation reaction. Many antibodies can be purchased already conjugated, which can save...you want is not available pre-conjugated, or is not available conjugated to your signaling molecule of...indirect detection method uses an unconjugated primary antibody and a conjugated secondary antibody specific...well plate. A conjugated primary antibody is used to bind to the antigen and the conjugate, typically HRP...indirect sandwich method, a conjugated secondary antibody is added. The conjugate is activated and the output...

...Bacterial (pET), Gateway GTPase Arl4c 10123 201/192 Gateway GTPase Arl4d 379 201 Gateway GTPase Arl5a 26225...(pET), Gateway GTPase Arl8a 127829 186 Bacterial (pET), Gateway GTPase Arl8b 55207 186 Gateway GTPase ...pcDNA3), Gateway GTPase Sar1a 56681 198 Bacterial (pET), Gateway GTPase Sar1b 51128 198 Gateway GTPase ...Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf4 378...Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf6 382...Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Mammalian (pEGFP-N3) Gateway GTPase Arl2 402...Mammalian (pcDNA3), Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl4a 10124...

...rather than two. Stephen Ekker Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple...compatible with Golden Gate TALEN and TAL Effector Kit 2.0. Stephen Ekker Platinum Gate TALEN Kit Platinum...activities in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination...VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena Torres-Padilla...destination vectors for Voytas Golden Gate-assembly kit. 47875 pTALYM4 Golden Gate TALEN 2.0 48705 pTAL7a Boris...System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning...Effector Engineering Reagents Assembly via serial ligation. Validated in zebrafish somatic cells. Keith Joung...

... constructs, based on the Golden Gate method. Golden Gate (GreenGate) Jan Lohmann 1000000076 GoldenBraid... Golden-Gate cloning of synthetic operons into chloroplast transformation vectors. Golden Gate (MoClo)... Scott Lenaghan 1000000241 GreenGate 2.0 Toolkit Compatible with GreenGate Cloning System (#1000000036...plasma membrane and nuclear markers. Golden Gate (GreenGate) Alexis Maizel 1000000250 MultiGreen Kit Modular...assembly of multi-gene expression vectors. Golden Gate (GreenGate) Wayne Parrott Additional Cloning and Synthetic...CRISPR Daniel Voytas 1000000241 GreenGate 2.0 Toolkit Compatible with GreenGate Cloning System (#1000000036...ID Kit Description Cloning Type PI 1000000036 GreenGate Cloning System Plasmids for rapidly assembling...

...-ccdB - Yeast Gateway expression vector (see more Gateway vectors) pCAP03... TRP1 Yeast pGBKCg - Gateway compatible Y2H vector LEU2 Yeast pGADT7-GW - Gateway compatible Y2H vector...vector - AD pGADCg - Gateway compatible Y2H vector BASTA Plant pGTQL1211YN - Gateway compatible BiFC vector... Gradia Lab plasmids for mammalian expression, Ligation Independent Cloning (LIC) pAG CMV Empty Puro -... Gradia Lab plasmids for bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial...RPS5A, Ubiquitin pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1...collection page Yeast GAL4, PGK, ADH1, ADE2, TRP1 Gateway destination vectors - Inducible...

...OPEN OPEN cACCTGCAGCagattGAAGAAGAGg cftr OZ517 and OZ518 OPEN OPEN gTGCAACCGCagctttTAGGACGGAt fox1 (a2bp1l...OPEN OPEN gCCCCTCAGCccagatgGGAGGAGATg dpf2 OZ531 and OZ532 OPEN OPEN cTGCTACTTCtggatGGAGAAACGa pitx2 OZ533...CODA CODA gTCCAGCAGCGGTGAGGATGAAGACg unc119b OZ581 and OZ582 CODA CODA tTGCTGCCGCGGATGCTGTGGCGGTTa cx52.9...half-site CACNA1F OZ507 and OZ508 OPEN OPEN gCGTGACCTCgctgatAAGAAGAGg xylt1 OZ509 and OZ510 OPEN OPEN cACCCTCTACaggacaGTAGCGGGTg...gpcr-2037) OZ535 and OZ536 OPEN OPEN aGCCATCATCaggttcaGGATGAGCCc Kif6 OZ537 and OZ538 OPEN OPEN aAGCTTCCTCttcattgGCTGACTGCa...Gene (sbds) OZ545 and OZ546 OPEN OPEN tGTTGCCGTCgtgagGATGAAGAAa CACNA1D (2of2) or cav1.3b OZ547 and OZ548...corepressor 2 OZ553 and OZ554 OPEN OPEN gCTCACCATCtgttggaTGCGATGGAa cdkn1a (p21) OZ555 and OZ556 OPEN OPEN...

...Gram-negative bacteria Philip Poole 14460 pOT2 Promoter activity Fluorescence (GFPuv) Gram-negative bacteria...plasmids suitable for replication in many gram-negative bacteria with different purification tag combinations...promoters and purification tags compatible with Golden Gate modular cloning (MoClo) for use in E. coli . Addgene... (Addgene #1000000222) is a collection of Golden Gate compatible vectors for bimolecular fluorescence ...activity GUS activity and fluorescence (mRFP1) Gram-negative bacteria Philip Poole 14473 pRU1156 Promoter activity...GUS activity and fluorescence (GFPmut3.1) Gram-negative bacteria Philip Poole 14471 pRU1144 Promoter activity... activity Fluorescence (mRFP1) Gram-negative bacteria Philip Poole 14462 pRU1097 Promoter activity Fluorescence...

...Insertion of 5' promoter/enhancer regions using Gateway cloning. Jorge Ferrer 16539 pBV-Luc Firefly Insertion...Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF) Firefly, Renilla Creating...212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription Debrya Groskreutz...® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc...® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2...® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X...® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 106253 pLS-mP-Luc Firefly...

...1 PX458_FOXP1_2 GATAD1 Human FLAG pFETCh_GATAD1 PX458_GATAD1_1 PX458_GATAD1_2 GATAD2A Human FLAG pFETCh_GATAD2A...pFETCh_GATAD2A PX458_GATAD2A_1 PX458_GATAD2A_2 HHEX Human FLAG pFETCh_HHEX PX458_HHEX_1 PX458_HHEX_2 HBP1...

...microscopy after negative staining. Empty vector particles can be identified after negative staining and ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...final vector preparation, the quantity of gram-negative bacterial endotoxin is ensured to be less than...containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a 0.45 µm polyethersulfone...possible, all plasmids used for viral production are propagated in the endA -mutated NEB Stable strain of E. ...

...expression, TRE-gateway, with puromycin selection. See Root Lab pLEX and pLIX Gateway backbones for additional.... Linzhao Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible... Tet-based inducible shRNA or cDNA expression, gateway plasmid, and constitutive hygromycin selection....variants. Iain Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression. Miguel...pULTRA versions . Malcolm Moore 25895 pLX301 3rd Gateway plasmid for CMV driven expression of cDNA. See ...

...contains pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and Signaling...Anderson Lab Plasmids and Phagemids Balazsi Lab Negative Autoregulation and Yeast-Mammalian Circuit Transfer...Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit...Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular...Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium...

...same reaction which ligates a promoter into an empty backbone can be repeated to ligate your gene of interest... 21) Prefix: GAATTC ATG AGATCT Prefix Enzymes: EcoR1 , BglII Suffix: T GGATCC TAA CTCGAG Suffix Enzymes...Enzymes: BamHI , Xhol Scar: GGATCT Features: Scar encodes Gly-Ser in-frame with prefix start codon; Employs...

...pMH-Halo tag - Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/C-Halo...engineered to covalently bind a synthetic substrate conjugated to an organic fluorophore or other small molecule...molecule. By using different ligand-dye conjugates, you can label one fusion protein with different colors...- Mammalian Expression pENTR4-SNAPf (w878-1) - Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf - Xenopus...- Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag - Bacterial...Neveu Lab MXS Chaining Kit Lindquist Lab Yeast Gateway Kit Reading and Resources Addgene Blog: Which Fluorescent...