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We narrowed to 302 results for: human dat

Showing: 21 - 40 of 302 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...editing. These edits can include mutations seen in human patients, protein tags, or loxP/FRT sites, among...germline editing, with the International Summit of Human Gene Editing convened in 2015 to discuss the ethics...plasmids for the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus...2013. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . ... Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu...Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A . 112(10):3002-7...DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human genome engineering via Cas9. Science . 339(6121)...

  2. p53 Pathway

    Type
    Collection
    ...p53 is the most frequently mutated gene (>50%) in human cancer; most of these mutations are missense mutations...mutagens like radiation or viruses (e.g., HPV, the human papillomavirus). These mutations interfere with .... Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget S, Khoury...45-53. PubMed PMID: 11780111 . p53 mutations in human cancers. Hollstein M, Sidransky D, Vogelstein B,...activated by cellular stresses like DNA damage, oxidative stress, and nutrient deprivation. p53 is activated...component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2...

  3. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... reveals specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. 2016 Jun 6. PubMed PMID: ...Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells." Nature Biotechnology (2016). PubMed PMID... RA, van der Oost J, Backofen R, Koonin EV. An updated evolutionary classification of CRISPR-Cas systems...Pages Check Out MpAgo from the Doudna Lab Find Validated gRNAs ...

  4. Brain Initiative Collection

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    Collection
    ...Initiative aims to revolutionize our knowledge of the human brain through the development and application of...cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter Edward Boyden 107712...pAAV-CAG-DIO-ChR2(H134R)-eYFP Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic...pAAV-syn-FLEX-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch...Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch...pAAV-syn-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct...core has 100% seq conservation between mouse and human Jonathan Ting 163509-PHPeB CN1839-rAAV-hSyn1-SYFP2...

  5. Antibody Plasmid Collection

    Type
    Collection
    ...display vector system for producing human antibodies Synthetic Human Antibodies: Selecting and Evolving...Beavil Cloning and mammalian expression of mouse and human IgG monoclonals with reduced immune effector functions...produced in-house and undergo application-specific validation and quality control by Addgene as well as by ...collection - monoclonal antibodies extensively validated for neuroscience research applications from the...

  6. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...Miller Felix D., Rist Wolfgang, and Lamla Thorsten. Human Gene Therapy Methods. August 2015, 26(4): 147-157...aliquot and store at -80 °C for long term. Sample Data Figure 1: Iodixanol gradient before ultracentrifugation...

  7. Zebrafish Plasmid Collection

    Type
    Collection
    ... stalwart research model. With more than 70% of human disease genes having an ortholog in zebrafish, this...has more recently emerged as a model system for human disease and translational research, including the...cancer, inflammation and infection, regeneration, human neurological disorders, microbiota studies, and ...Model Organism Database (ZFIN) - A curated database of genetic, genomic, and developmental data for zebrafish...CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish...expression atlas - A zebrafish-specific gene expression database that provides information about when genes are...active in developing embryos. zfRegeneration - A database for gene expression profiling during regeneration...

  8. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...) CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression plasmids for use...Gene Guide RNA expression plasmids for endogenous human genes Guide RNA expression plasmids for endogenous...described gRNA and Cas9 expression vectors and validated the use of RNAs produced by these vectors for ...

  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... beta-3 Human, Mouse IgG1 Rabbit Anti-Integrin alpha L [IPI-TS2/4] Integrin alpha-L Human IgG1 Rabbit...alpha-L Human IgG1 Rabbit Anti-Integrin alpha M [IPI-M1/70] Integrin alpha-M Mouse, Human IgG1 Rabbit...Integrin alpha-M Human IgG1 Rabbit Anti-Integrin alpha M [IPI-CBRM1/29] Integrin alpha-M Human IgG1 Rabbit...alpha-M Mouse, Human IgG2a Rat Anti-Integrin alpha M [IPI-CBRM1/5] Integrin alpha-M Human IgG2a Mouse...Integrin alpha-M Human IgG2a Mouse Anti-Integrin alpha M [IPI-LM2/1] Integrin alpha-M Human IgG2a Mouse...environments. Found on nearly every cell in the human body, integrins are key to a diverse array of processes..., and others are rat or mouse IgG2a. Additional human IgG1s are also available on demand. The 11 IPI synthetic...

  10. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...stability in human plasma The last trick GeoCas9 has up its sleeve is its increased longevity in human serum....Figure 2: Human plasma longevity of SpCas9 vs GeoCas9. SpCas9 or GeoCas9 RNPs were incubated in human plasma... in diluted human plasma. GeoCas9 remains active after being incubated in up to 30% human plasma. ...increased thermostability also had a longer lifetime in human blood. To test if this was also true for GeoCas9...al incubated SpCas9 and GeoCas9 RNPs in diluted human plasma for 8 hours at 37°C. Substrate was then added... reaction conditions. Finally, its longevity in human serum may allow for more efficient genome editing...most excited about? Thermostability? Longevity in human plasma? Something we haven’t thought of yet? Let...

  11. Adenovirus Plasmids

    Type
    Collection
    ... to cause mild to severe respiratory disease in humans. Advantages of adenoviruses in research include...Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be...plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have inserts...for the AdenoBuilder genome assembly system; consolidates the regions present in pAd5-B6 ∆E3-GFP and pAd5...

  12. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...similarities with humans. However, rats have some key differences in physiology, intelligence, and human correspondence...corresponds with human diseases and conditions. For example, the rat is frequently used to study human cardiovascular...sequencing and concluded that nearly all disease-linked human genes have counterparts in the rat (Gibbs, et al..., studying the axolotl may reveal the potential humans have for a similar degree of regeneration.  In ...phenotypic changes that are similar to those observed in humans. Thus, understanding their life cycle and processes...regenerative abilities is expected to shed light on human aging pathological developments (Vieira et al., ...patterns are conserved, making them great models for human disease research. One of their most attractive features...

  13. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...far enough along, scientifically, to be doing in humans; We have not had a broad, public conversation or... scientifically unproven technologies to edit a human embryo, or have a woman carry an edited embryo to...international moratorium on utilizing CRISPR/Cas9 in humans. This topic was to be revisited at meetings when...social and ethical issues of genome editing and human reproduction, the relevant document for the question... research, animal models, and several stages of human clinical trials. This model also demonstrates that...always been people who unethically experiment on humans, and they are not remembered kindly. However, despite...was recognized as an unethical experimentation on human subjects, utilizing a scientific technique not proven...

  14. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...viral proteins in human cells and used these viral “bait” proteins to fish for human “prey” proteins that...rely on the help of human proteins in their lifecycle, identifying these potential human helpers of SARS-...Leary (UCSF). Our approach is to discover key human proteins that can be targeted (hopefully by existing...BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction network in which we expressed 26 of ... with the virus. As a result, we identified 332 human proteins that the virus interacts with in cells....therapeutic monkeywrench into the virus’ hijacking of human cells. Even better, more than half the drugs hitting... of understanding how SARS-CoV-2 interacts with human proteins. For example, we used HEK293T cells in ...

  15. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...understand how each human TF can contribute to differentiation, the Zhang lab transduced human embryonic stem...accompanying expression atlas, including over 3,500 human TF isoforms, to help researchers understand this... library The transcription factor library The human genome encodes approximately 1,800 TFs, with >3,...packaged in lentiviral particles and used to transduce human embryonic stem cells. Transduced stem cells were...to that of reference cell types generated by the human fetal expression atlas. Computational analysis was...tool to be used in this capacity. Similar to the Human TFome Library, the MORF library can be used with...library amplification Zhang Lab CRISPR Plasmids The Human TFome Library  ...

  16. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...time of sampling if available. STR profiling for human, mouse, and dog cell lines is included when available...Using the data provided by the researcher, Cellosaurus was able to both update their database and sent ...Rigor and reproducibility of scientific data depends on validated reagents along with clear documentation...called Cellosaurus, a growing cell line information database describing nearly 145,000 cell lines. Cellosaurus...2 research, or adenovirus packaging cell lines dataset collections.  Depending on available information...cell line you’ve used? Submit your manuscript or data to Cellosaurus via [email protected] community...sent the updated information to the cell line distributor. A success all around! If you’re excited about ...

  17. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...science,” said Guillaume Bourque, a Professor in Human Genetics at McGill University. “There is a need ... new, centralized databases and centers to support the sharing of materials and data associated with COVID...into COVID-19 (like here at Addgene!), and new databases are supporting their access to useful resources...internationally COVID-19 Resources Canada is a database that provides information about reagents, funding...co-founded the COVID-19 Resources Canada site. To date, the portal has recruited 3,218 Canadian volunteers...supports collaborations at Rutgers, but also lists data and experimental resources that are useful to any...for COVID-19 research in their field including datasets and platforms for discussing new developments,...

  18. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...have a human counterpart, enabling study of human-relevant pathways (Liu et al., 2017). Beyond human relevance...problematic for human administration. Thus, the production of glycoproteins for human therapies, such ...have its genome fully sequenced There are several human disease models established in yeast, including a...antibodies, is possible in K. phaffii since it produces ‘human-like’ glycosylated proteins. Peroxisome studies..., Wei, et al., From Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical...sequestering certain enzymes and performing specific oxidative reactions. They are found in all eukaryotic cells...tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation K. phaffii...

  19. What the HEK?

    Type
    Blog Post
    ...proteins are necessary, HEKs are human in origin, so they eliminate non-human PTMs which is a concern with...al., Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293...Ever wondered where human embryonic kidney (HEK) cells originated from? Or why they are such a commonly... What the HEK is wrong with it?   All cultured human cell lines are susceptible to contamination by mycoplasma.... Are HEKs for you?   If you are looking for a human cell line model, HEKs might be a great option! Especially...include receptor biology, ion channels, or involve human protein preparations with PTMs. Why the HEK not?...293 cells are tumorigenic, which makes them a candidate cell line model for cancer-specific research. ...

  20. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ... synthetic promoter AAVs in mice, non-human primates and humans. Nature Neuroscience, 22, 1345-1356. https...for targeting the same cell class in humans (R=0.67).  A database of the microscopy images of all AAVs...synthetic promoters performance in mouse, monkey, and human retinas Next, the 113 AAV promoters that were active...retinas were injected into macaques and postmortem human retinas. About 25% of these AAV synthetic promoters...promoters successfully targeted cells in monkey and human retinas. By comparing the performance of promoters...targeting the same cell class in NHPs (R=0.38) and humans (R=0.32), but the odds of that promoter targeting...chances of a promoter expressing in primate and human retinas but not in mice was low (R=0.12), suggesting...
Showing: 21 - 40 of 302 results