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We narrowed to 550 results for: nol

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Showing: 21 - 40 of 550 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also have an older system using two...express up to three sgRNAs (Swiech et al., Nature Biotechnology 2014). Full references are below. Available ...mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of genetic elements...Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;31(9):827-32. doi: 10.1038/nbt.2647...Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6. doi: 10.1038/nbt.3055...

  2. AAV Molecular Tools

    Type
    Collection
    ... promoter in a 'Cre-on' dependent manner. 1 Don Arnold 169128 pAAV-DIO-EF1α-mKate2-PDE4D3-Cat EF1a-driven...viruses infected in postsynaptic cells. 5, 8 Don Arnold 232352 pAAV-DIO-ATLASsnFlp Syn-driven, Cre-dependent...fDIO-Reporter viruses infected in postsynaptic cells. 8 Don Arnold 232353 pAAV-DIO-BACE-HA Syn-driven, Cre-dependent...expressed (1:1) with either ATLAS protein. 8 Don Arnold Don’t See What You’re Looking For? Our Packaged...

  3. Western Blot

    Type
    Protocol
    ...15 min in 20% ethanol in deionized water. To prepare 20% ethanol, dilute 2 mL of ethanol into 8 mL of ...Microcentrifuge tubes BCA Assay, Thermo Fisher 23227 β-mercaptoethanol 4X protein loading buffer Precast SDS-PAGE ...-PAGE running buffer Prestained protein ladder Ethanol iBlot 2 PVDF Mini Stack, Thermo Fisher IB24002 ...sample for loading as follows: Add 10% v/v β-mercaptoethanol to the 4X protein loading buffer. Dilute 4X...

  4. Institute for Protein Innovation

    Type
    Collection
    ...opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...cone receptors. Developed using yeast display technology, these antibodies enable high-confidence targeting...synaptic cleft. Developed using yeast display technology, these antibodies provide researchers with powerful...

  5. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England...fixed cells. The plasmids associated with this technology are available at Addgene for distribution to ...NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase...

  6. Antibody Plasmid Collection

    Type
    Collection
    ...monoclonal antibody fragments by phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas...and utilisation of recombinant antibodies. BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system...selected from Tomlinson I+J phagemid libraries. Immunol Lett. 2015 Oct;167(2):95-102. Joanna Bereta Sybody...

  7. Microbiology Resources

    Type
    Collection
    ...units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression...CRISPR Guide for a general introduction to CRISPR technology. Check out our CRISPR reference page for CRISPR...resources Bacterial CRISPR Resources The CRISPR technology was developed from bacterial immune systems, ...

  8. Zebrafish Plasmid Collection

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR resources for a full ...zebrafish resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - ...- A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines ...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...encoded calcium indicator for in vivo imaging. Nat Biotechnol. 2021 Mar;39(3):368-377. Vladislav Verkhusha ...pentose and disaccharide accumulation in bacteria. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. Wolf Frommer Maltose...indicator for in vitro and in vivo studies. Nat Biotechnol. 2018 Sep;36(8):726-737. Yulong Li Acetylcholine...imaging of endocannabinoid dynamics in vivo. Nat Biotechnol. 2021 Nov 11. pii: 10.1038/s41587-021-01074-4...measurements of membrane charges in live cells. Nat Biotechnol. 2017 Mar 13. Katharina Gaus Endo/Exocytosis ...

  10. Lentivirus Plasmids

    Type
    Collection
    ...interest) can be cloned into the plasmid. Garry Nolan 12257 pWPXL 2nd EF-1alpha-driven constitutive transgene...vector system. Expresses gag-pol and rev . Garry Nolan 8455 pCMV-dR8.2 dvpr 2nd Contains gag , pol , rev...recommended for the FELIX (FIV-based) system. Garry Nolan 8454 pCMV-VSV-G VSV-G-expressing envelope plasmid...

  11. Rett Syndrome

    Type
    Collection
    ... Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach for precise...CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources for a full ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV Huntington's Arnold Boersma 175061 pQ39-VC HTT Venus, mCherry CMV Huntington's Arnold Boersma 175062...mCherry CMV Huntington's Arnold Boersma 175064 pQ79 HTT mCherry CMV Huntington's Arnold Boersma 175066 pVC-...mCherry CMV ALS Arnold Boersma 175067 pVC-FUS-R521C FUS Venus, mCherry, HA CMV ALS Arnold Boersma 175068...Corporation 237172 EIF2AK2(252-551)-NanoLuc Fusion Vector EIF2AK2 NanoLuc CMV VWM disease Promega Corporation...2A Tau WT WPRE MAPT CBA Parkinson's, FTD Matthew Nolan 138134 pT7NT*-Bri2 113-231 R221E ITM2B NT T7 Familial...175068 pFUS-WT-VC FUS Venus, mCherry, HA CMV ALS Arnold Boersma 175307 pH6HTC_HNRNPA1-Halo HNRNPA1 Halo, His... Arrowsmith 211083 pNLF1-C_EIF2B2:1-351 EIF2B2 NanoLuc CMV VWM, CACH Cheryl Arrowsmith 211112 pBiT3.1-...

  13. Plan Your Experiment

    Type
    Collection
    ...more information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different... for CRISPR-Based genome editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech... analysis of CRISPR–Cas experiments. Nature Biotechnology , 38 (7), 813–823. https://doi.org/10.1038/s41587...

  14. Zinc Finger Consortium Reagents

    Type
    Collection
    ...research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas... nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...

  15. Water Bath Protocol

    Type
    Protocol
    ...the lab. Equipment Water Bath 10% bleach or 70% ethanol Distilled water Disinfectant Thermometer Water ...water. You may use a 10% bleach solution or a 70% ethanol solution to wipe down the inside of the water bath...marker that is also resistant to chemicals such as ethanol. Writing on the tops of the tubes and lids of items...

  16. Video Library

    Type
    Protocol
    ...produced in mammalian cells AAV Purification by Iodixanol Gradient Ultracentrifugation Protocol Mulitchannel...preparation. AAV Titration by qPCR Using SYBR Green Technology Protocol Agarose Gel Electrophoresis Gel electrophoresis... process when requesting samples from Addgene Technology Transfer How to Order A step-by-step walkthrough...

  17. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...use Watch the Video! DNA Purification Miniprep, phenol-chloroform extract, and precipitate DNA DNA Quantification...the target of your choice AAV Purification by Iodixanol Gradient Ultracentrifugation Purify adeno-associated...Video! AAV Titration by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer of your...

  18. Distribution to Industry

    Type
    Collection
    ...Ordering and MTA Information Featured Collections NEW NanoLuc and HaloTag Fusions from Promega COVID-19 SARS-...distribution. Please see the Sample Agreements on our Technology Transfer Information page for more information...

  19. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Production in HEK293T Cells AAV Purification by Iodixanol Gradient Ultracentrifugation AAV Titration by ... ddPCR AAV Titration by qPCR Using SYBR Green Technology Content last reviewed: 10 September 2025 Do you...

  20. Open Enzyme Collection

    Type
    Collection
    ...biology, DNA origami, immunology, biochemistry, and more. The foundational technologies enabled by this collection...
Showing: 21 - 40 of 550 results