We narrowed to 46 results for: pSC
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TypeBlog Post...on transgene design and delivery. They generated hPSC lines with AAVS1-integrated, inducible, and fluorescent...
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Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...basal forebrain cholinergic neurons derived from iPSCs. From Rohn et al. 2018; see figure 3 for full details... -
Human Germline Editing Using CRISPR
TypeBlog Post...They observed higher targeting efficiency than in iPSCs, but with the caveat of mosaicism. To circumvent... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...other photoswitchable kinases: psMEK2, psRaf1, and psCDK5. MEK2 is a serine/threonine kinase with a similar... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post... of many human malignancies. Additionally, the Tapscott Lab identified regulators of DUX4, a transcriptional... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...of AAV mediated genome editing in human ESCs and iPSCs from ~0.2% to ~1.5%. In more recent work, researchers... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...certain processes, like cellular reprogramming of iPSCs, more efficient. In general, epigenetic chromatin... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...in Lung Epithelial Cells Generated from Patient iPSCs.,” Cell Rep., vol. 12, no. 9, pp. 1385–90, Aug. ... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...to viral vector delivery of transgenes, like for iPSC reprogramming and gene and cell therapy, and have... -
Plasmids for Stem Cell Research
TypeCollection... cells (iPSCs) in both mouse and human cells in 2006 and 2007, respectively. To create iPSCs, fully-differentiated...developed many approaches to generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically... can be used to create iPSCs or another cell type. You can also search the iPSC Neurodegenerative Disease...Mol Syst Biol. 2014 Nov 17;10(11):760. Weiss iPSCs & iPSC-NPCs Neurons Lentiviral Human Rapid Ngn2-induction...for the generation and study of stem cells, from iPSC generation to directed differentiation. Plasmid...development, disease modeling, and regenerative medicine. iPSCs, much like their ES cell cousins, have the hallmarks...the capacity to give rise to an entire organism. iPSC technology removes the possible ethical concerns... -
Rett Syndrome
TypeCollection...17289941 2010 - Allyson Muotri's lab develops the first iPSC model for Rett syndrome. (Link opens in a new window...Cell Line Models Induced pluripotent stem cells (iPSCs) are a powerful system for generating Rett syndrome-specific...Patient-specific lines (Link opens in a new window) RSRT iPSC lines and isogenic controls , patient-specific Infinity...Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link opens in a new window) PMID: 26944080 (Link...) Alysson Muotri Q83X C247T Q83X M Fibroblasts & iPSC (Link opens in a new window) PMID: 26944080 (Link...Muotri c.806delG 806delG G269Afs*20 M Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold...Gold AN_BU 808delC Arg270Glufs*19 F Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold... -
Neurodegeneration Research Collection
TypeCollection...2023 May 4. See More iPSC Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into... Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins...expression of aggregation-prone proteins with the iPSC toolbox . Lam et al. bioRxiv. 2022 Dec 2. Use PiggyBac... -
Neurodegeneration Plasmid Collection
TypeCollection...Diane Lipscombe 26578 CaV2.1 (pcDNA6) CACNA1A His, V5 CMV Spinocerebellar ataxia 7 Diane Lipscombe 26674...Fu 189554 pSCM167.2-Venus-VF-hADAM10(aa1-213) ADAM10 VF Alzheimer's Haian Fu 189555 pSCM167-Venus-VF-hADAM10... 189556 pSCM167.2-Venus-VF-hADAM10(aa455-550) ADAM10 VF Alzheimer's Haian Fu 189557 pSCM167.2-Venus-VF-hADAM10...Fu 189558 pSCM167.2-Venus-VF-hADAM10(aa1-654) ADAM10 VF Alzheimer's Haian Fu 189559 pSCM167-Venus-VF-hADAM10... 189560 pSCM167-Venus-VF-hADAM10(aa220-654) ADAM10 VF CMV Alzheimer's Haian Fu 189561 pSCM167-Venus-VF-hADAM10...-N'His SOD1 His T7 ALS Elizabeth Fisher 118744 pScarlet-Kif5a KIF5A mScarlet CMV ALS Beverly Koller 118745...mEGFP-OPTN OPTN GFP ALS Michael Lazarou 189552 pSCM167.2-Venus-VF-hADAM10 ADAM10 VF UbC Alzheimer's Haian... -
Bacterial Expression Systems
TypeCollection...Propionate Escherichia coli Jay Keasling 113634 pSCrhaB2 PrhaB Rhamnose Burkholderia cenocepacia Miguel...nm) Escherichia coli Christopher Voigt 164226 pSCrhaB2plus PrhaBAD Rhamnose Burkholderia sp. Silvia Cardona...Fluorescence (mRFP1) Escherichia coli Mary Dunlop 124605 pSCM001 Cytoplasmic pH Fluorescence (mCherry) Escherichia... -
Tetracycline Inducible Expression
TypeCollection...differentiating iPSCs into glutamatergic neurons via human NGN2 expression. See more from the iPSC Neurodegenerative...dox-inducible human NGN2 into AAVS1 safe harbor site, for iPSC differentiation into cortical neurons Michael Ward... -
Allen Institute for Cell Science Plasmid Collection
TypeCollection...fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines...cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the Allen Institute... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...capable of promoting induced-pluripotent stem cells (iPSCs) to take on a “neuron-like” phenotype when targeting... -
Luciferase Plasmid Collection
TypeCollection...or tet-inducible luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression...expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc Renilla CAG Vector for packaging self-... -
Depositor Collections
TypeCollection...Research Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative Disease Initiative (iNDI) Collection...