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We narrowed to 799 results for: tec

Showing: 21 - 40 of 799 results

  1. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...proteins to study. These are highly useful for the biotech industry, which is in the process of capitalizing...other modifications to their bases in order to protect their DNA from host cell nucleases (4). To further...event that genome sequencing can be done, this technique can also help pinpoint the genes within a sequenced...live cells The following is a description of a technique published by Simpson, Sacher, and Szymanski in...membranes well with PBST to remove unbound cells. To detect bound cells, simply lay each membrane on an agar...

  2. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ... distributed plasmid technologies pSV-PGC1 - Ok, it's not actually a technology, but pSV-PGC1 was Addgene's...institutions. This has given us a unique window into technology trends in the life sciences. In this post, we'll...give you an inside look at the Top 10 plasmid technologies distributed through Addgene over our first 10...genetically encoded calcium indicators were the hot technology of the year in our collection. CRISPR/Cas9 - ...Year of the CRISPR." The newest genome editing technology has been used and modified by so many labs and...

  3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ....   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay Sensitivity...R​eporter un​LOCK​ing) technique Sensitivity: Single molecule per milliliter can be detected – in the zeptomolar...-1 and SARS-CoV-2 detection, AIOD-CRISPR without preamplification was able to detect as low as 1.2 copies...visualization. In this technique, however, instead of using lateral flow strips to detect the cleavage of fluorescently...: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique that uses FnCas9, a protein that...CY (2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587...times, have led researchers to turn to other technologies like CRISPR. Figure 1: An overview of...

  6. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...do this.” Biotech startups are only successful if they are 100X better than current technology and get ...Capital in Biotech  - Posted on Forbes.com, contributed by Bruce Booth How to Find a Job in Biotech and Resume...developed during graduate school and turn it into a biotech company. Transitioning an idea from academia to...Sample6 is an enrichment-free bacterial pathogen detection system that uses synthetic biology to target and...concepts to engineer bacteriophages and use those to detect unwanted bacteria. How did graduate school help...a succesful entrepreneur? As a co-founder of a biotech startup, Mike explained, his career transitioned... the best product-market fit for this set of technologies, effectively communicating the advantages embodied...

  7. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...the limited technologies available. Over the past few years, however, remarkable technological advances ...revolution in modern neuroscience, an era in which technologies like FLEx and optogenetics meet, allowing scientists...mammalian neurons and alter synaptic events: a new technology was born, and the term “optogenetics” was coined...fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing cells. The resulting fusion gene... functional topography of the neural circuit architecture of the brain regions associated with motivated... able to distribute materials containing FLEx technology. Read the genOway press release for more information...Deisseroth, Karl, et al. "Next-generation optical technologies for illuminating genetically targeted brain ...

  8. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...Branon et al. Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way we detect viral outbreaks ...often escape detection. The Alexander Green lab found a solution: a low-cost, cell-free detection assay that...isothermally, and detected through a toehold switch, an RNA sensor that can be used to detect the presence ...toehold switches to detect Zika virus (available at Addgene). The plasmids for detecting norovirus will be... have now applied the same directed evolution technique to the biotin ligase from which BioID was derived... and BioID. In the Ting Lab’s recent Nature Biotechnology paper, they describe TurboID and miniTurbo, ...contributed by Izzy Mueller  Listen to the viral detection podcast segment As the primary cause for both...

  9. Promega Plasmid Collection

    Type
    Collection
    ...for additional detection options. HiBiT technology's exceptional dynamic range can detect low-abundance...Industry Plasmid Technology Overview This collection includes specialized Promega technologies that support...about NanoLuc technology (Link opens in a new window) from Promega Read about HaloTag technology (Link opens...NanoLuc, HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins. Depositor... Ordering & Availability All Plasmids Plasmid Technology Overview Additional Resources (Link opens in ...expression analysis and protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc...support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase...

  10. CRISPR Guide

    Type
    Collection
    ... range of genome engineering techniques . Collectively, these techniques are referred to as CRISPR ( C...molecular detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform... to SHERLOCKv2 to detect multiple targets. For example, Cas12 can be added to detect the presence of infectious...have an interesting adaptive immune response that detects and eliminates foreign DNA, which scientists have...egularly I nterspaced S hort P alindromic R epeat) technologies. Before CRISPR, genome engineering approaches...CRISPR one of the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens...CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids. Acr family...

  11. Antibody Guide

    Type
    Collection
    ...amplification through the indirect detection method. In the direct detection method, the signaling molecule.... Figure 3: A western blot detecting proteins using the indirect detection method, with a secondary conjugated...conjugated, it is often called the detection antibody. If using an indirect detection method, add a secondary antibody...microscopy techniques used, so be sure to read materials specific to your assay. Most IF techniques use a ...terms, summarize antibody production and storage techniques, and explain common applications. Science...secretions (e.g. tears, saliva, mucus) where it protects mucosal membranes Dimer with a valency of 4 IgA1...function is unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving...

  12. COVID-19 Resources

    Type
    Collection
    ...Tools SHERLOCK and DETECTR - Simple, portable, and inexpensive platforms to reliably detect nucleic acids ...for utilizing SHERLOCK to detect coronavirus - Enabling coronavirus detection using CRISPR-Cas13: Open-... ) for detection of SARS-CoV-2: A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV...Reporter (DETECTR). Mammoth Biosciences has published information and protocols for utilizing DETECTR to detect...Reverse Transcriptases, etc. that may be useful for detection assays and more. Pooled Libraries : Pooled libraries... Blog: Finding nucleic acids with SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized...detect coronavirus - A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR...

  13. TALEN Guide

    Type
    Collection
    ...TALEN technology and using TALEN plasmids... TALENs TALEN Guide Addgene's Guide to TALEN Technologies You ...'s December 2011 Newsletter Overview of TALEN Technology Imagine being able to manipulate a specific region..., zinc finger (ZF) arrays have been the go-to technology for targeting enzymes and other useful protein.... With the template for studying this type of technology already laid by the zinc finger community ( see...DNA regions into a single construct would be technically challenging. Thanks to efforts by the Bogdanove...Addgene. This kit, first described in Nature Biotechnology in August, uses a serial ligation protocol to...

  14. AAVED

    Type
    Collection
    ...this technology is used for targeted neuronal manipulation. Practical tips for using the technology in ...Education and Development (AAVED) Series AAV Technologies and Methods for Targeted Neuronal Manipulation...the guide. We hope that this guide will improve technology adoption and scientific outcomes. Event Details...Cepko 2:45 PM Break 3:00 PM (Group Discussion) Technologies and Resources Missing in the Field Kenton Woodard...presentation is to: Give a general overview of the technology and how it can be used for targeted neuronal ...focused on: Potential combination with other technologies to achieve different experimental goals. How... manipulation? Best practices for using this techonology in the lab (main issues/factors that researchers...

  15. Fluorescent Protein Guide: Biosensors

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    Collection
    ...sensors Single-color, ratiometric biosensors for detecting signaling activities in live cells. eLife. 2018...or Cre-dependent) A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo. Neuron...Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities. Nat Cell Biol. 2018 ...sensor Single-color, ratiometric biosensors for detecting signaling activities in live cells. eLife. 2018...pentose and disaccharide accumulation in bacteria. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. Wolf Frommer Maltose...intracellular tryptophan levels and flux Nanosensor detection of an immunoregulatory tryptophan influx/kynurenine...indicator for in vitro and in vivo studies. Nat Biotechnol. 2018 Sep;36(8):726-737. Yulong Li Acetylcholine...

  16. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... genome-wide screening. CRISPR Technological Advances CRISPR technology development has occurred at a ...various biological and translational technologies. Biotechnology companies are exploring therapeutic applications...applications, eclipsing past genome engineering technologies like zinc finger nucleases (ZFNs) and TALENs...Scientists have also developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors... it’s clear that CRISPR is a truly disruptive technology for basic research. The beauty of CRISPR is that...nuclease specificity using truncated guide RNAs. Nat Biotechnol . 32(3):279-84. PMID: 24463574 Fujita T, Fujii.... High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature . 529(...

  17. TALEN Engineering

    Type
    Collection
    ...Zebrafish ( Sander et al., Nat Biotechnol. 2011 ) Rats ( Tesson et al., Nat Biotechnol. 2011 ) Human somatic cells...cells ( Miller et al., Nat Biotechnol. 2011 ; Reyon and Tsai et al., Nat Biotechnol 2012 ) Human pluripotent...Ligation) assembly method (Sander et al., Nat Biotechnol. 2011), the REAL-Fast assembly method (Reyon ...) assembly method (Reyon and Tsai et al., Nat Biotechnol 2012). All three of these platforms assemble ...assemble TAL effector repeat arrays on a single architectural framework and the DNAs produced by all three methods...pluripotent stem cells ( Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...son of sevenless homolog 2 (Drosophila) FLJ25596 TEC tec protein tyrosine kinase MGC126760, MGC126762, PSCTK4...subfamily 4, group A, member 3 CHN, CSMF, MINOR, NOR1, TEC NR5A1 nuclear receptor subfamily 5, group A, member...potential pathogens each day. Their ability to protect against infection depends on two systems that work... have been infected by a foreign pathogen. They detect infected cells via receptors such as KIR and NKG2D... (C-C motif) ligand 25 Ckb15, MGC150327, SCYA25, TECK CCL26 chemokine (C-C motif) ligand 26 IMAC, MGC126714...

  19. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1* ( 188537 ) is a ROP minus plasmid...by tandem mass spectrometry (Barber et al, Nat. Biotech. 2018). This phosphosite library was generated ...split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent protein-peptide interactions...interactions (“mode #2” expression, Barber et al, Nat. Biotech. 2018). The phosphosite library is fused to the...kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library ...Gerstein M, Sampson JR, Isaacs FJ, Rinehart J. Nat Biotechnol. 2018 Aug;36(7):638-644. PubMed (Link opens in...

  20. Bacterial Expression Systems

    Type
    Collection
    ...tags, which can also later be used for protein detection in western blots, or removed using protease cleavage...bimolecular fluorescence complementation (BiFC) are techniques that have been widely used to study protein–protein...bring both FPs together and you will be able to detect fluorescence both from the donor and the acceptor...Reporter Plasmids Reporter plasmids can be used to detect events and molecules in and around a bacterium....small molecule, the reporter plasmid can be used to detect the presence of that small molecule and sometimes...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors...
Showing: 21 - 40 of 799 results