...Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids from Serotypes 1, 2, and 5 Display Differential...

...with the award. We are often more qualified and capable than we know or believe we are. Funded Research...

...Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological...

... a chosen focal volume and thus the microscope captures only fluorescence coming from this volume. The...

...photons are being emitted, making it easier to capture a clear image above background. The majority of...

...hope that these perspectives, the optimism they encapsulate, and the solutions they promote help motivate...

...builds on the YTK and adds several additional capabilities, including conditional gene expression, multiplexed...

...where primary and secondary antibodies are used to capture the protein of interest and detect its presence...

...A., and Kent Redman. "British anti-Lewisite (dimercaprol): an amazing history." Annals of emergency medicine...

... of a regular nucleotide for broader pairing capabilities. Taking into consideration the information above...

...bath, and be sure to know what the maximum fill capacity is. Look for a “fill” line inside the water bath...

...to ABE7.10. ABE8s have an improved base editing capacity, even at sites previously difficult to target....

... is generally accomplished by fusing an enzyme capable of converting one base to another to dCas9. The...

... others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...by the Feng Zhang lab ) combine the large-scale capacity of transposases with the precise DNA-targeting...programmability for different genomic loci, and the capability to detect multiple targets simultaneously. CRISPR...and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian Xu lab , utilizes... Cas12a also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single...carrying an ADAR truncation that retain RNA editing capabilities but are small enough to be packaged in AAV particles...two nuclease domains inactivated. This enzyme is capable of cleaving only one strand of target dsDNA. Off-target...

... of and gently in contact with the container (capillary action between the container and the liquid will...

...vol) N-laurosylsarcosine (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol® or similar product such as TRI Reagent...

...overloaded. If you find that your pellet outstrips the capacity listed in the Maxiprep protocol, scale the reagent...

...two components results in a transgene packaging capacity of >8 Kb. Constructs contain left and right arms...transgenes, the use of pAdEasy-2 can increase the capacity of the adenoviral vector. pAdEasy-2 does not contain... immune evasion, deleted to increase packaging capacity E4 Essential for viral transcription; Supplied...

... by the immune system, antibodies are proteins capable of highly specific binding to other proteins. Antibody-based...conjugated to a signaling molecule, can be used to capture, quantify, and identify proteins of interest. Read...

... total, and the stuffer insert is shown in all capital letters. I.2. Recipes Luria Broth Agar (LB agar...