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Showing: 381 - 400 of 416 results
  1. Finding Your Science Policy Path

    Type
    Blog Post
    ...writing for the Journal of Science Policy and Governance.  Get involved! The best way to learn about science...
  2. AAV Production in HEK293 Cells

    Type
    Protocol
    ...cells. Pass the cells twice a week during the maintenance phase and do not allow cells to reach 100% confluence...
  3. AAV Production in HEK293 Cells

    Type
    Protocol
    ...cells. Pass the cells twice a week during the maintenance phase and do not allow cells to reach 100% confluence...
  4. Typing CRISPR Systems

    Type
    Blog Post
    ...https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K. S., Demircioglu...
  5. Adenovirus Guide

    Type
    Guide
    ...in a higher background of non-recombinant adenoviral plasmids. Recombinant adenoviral plasmids are then...vectors for recombinant adenoviral vector production that do not contain GFP. Recombinant adenoviral vector...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type adenoviruses have...have been modified by researchers to create recombinant adenoviral (rAdV) vectors in order to deliver genetic...recombination have not occurred. Once verified, the recombinant plasmid is linearized with PacI to create a linear.... Common Uses of Adenoviral Vectors Although recombinant adeno-associated viral (rAAV) vectors are often...
  6. Adenovirus Guide

    Type
    Guide
    ...in a higher background of non-recombinant adenoviral plasmids. Recombinant adenoviral plasmids are then...vectors for recombinant adenoviral vector production that do not contain GFP. Recombinant adenoviral vector...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type adenoviruses have...have been modified by researchers to create recombinant adenoviral (rAdV) vectors in order to deliver genetic...recombination have not occurred. Once verified, the recombinant plasmid is linearized with PacI to create a linear.... Common Uses of Adenoviral Vectors Although recombinant adeno-associated viral (rAAV) vectors are often...
  7. Antibody Guide

    Type
    Guide
    ...Antibodies produced this way are called recombinant antibodies. Recombinant antibodies are created by cloning... time and labor intensive. Recombinant expression validation A recombinant expression strategy is used...cultured immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals... of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced from plasmids...abundant isotype in blood, also found in tissues; predominant isotype in secondary immune response Monomer ...Single-domain antibodies (sdAbs), also called nanobodies, are ~12-15 kD single heavy domain fragments ...Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared...
  8. Antibody Guide

    Type
    Guide
    ...Antibodies produced this way are called recombinant antibodies. Recombinant antibodies are created by cloning... time and labor intensive. Recombinant expression validation A recombinant expression strategy is used...cultured immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals... of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced from plasmids...abundant isotype in blood, also found in tissues; predominant isotype in secondary immune response Monomer ...Single-domain antibodies (sdAbs), also called nanobodies, are ~12-15 kD single heavy domain fragments ...Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared...
  9. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Guide . Recombinant AAV Vectors Wild-type AAVs have been modified by researchers to create recombinant adeno-associated...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...in order to deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the ...Created with BioRender.com. Genome Integration Recombinant AAVs are generally considered to not integrate...cystic fibrosis and cancer. Neuronal Tracing Recombinant AAV vectors have been widely used by neuroscientists...P. J., Mandel, R. J., & Muzyczka, N. (2004). Recombinant AAV viral vectors pseudotyped with viral capsids...sites in AAV virions during AAV wild-type and recombinant AAV vector production . Journal of Virology, ...
  10. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Guide . Recombinant AAV Vectors Wild-type AAVs have been modified by researchers to create recombinant adeno-associated...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...in order to deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the ...Created with BioRender.com. Genome Integration Recombinant AAVs are generally considered to not integrate...cystic fibrosis and cancer. Neuronal Tracing Recombinant AAV vectors have been widely used by neuroscientists...P. J., Mandel, R. J., & Muzyczka, N. (2004). Recombinant AAV viral vectors pseudotyped with viral capsids...sites in AAV virions during AAV wild-type and recombinant AAV vector production . Journal of Virology, ...
Showing: 381 - 400 of 416 results