We narrowed to 416 results for: nans
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TypeBlog Post...Taylor et al., 2016; Hooper and Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become ...
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An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...Andy Fire’s lab used oligo-mediated knock-in of a dominant mutation, known as co-conversion (Arribere et ... -
Finding Your Science Policy Path
TypeBlog Post...writing for the Journal of Science Policy and Governance. Get involved! The best way to learn about science... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...: managerial duties ex: order materials, lab maintenance Administrative assistant: assists PI with admin... -
AAV Production in HEK293 Cells
TypeProtocol...cells. Pass the cells twice a week during the maintenance phase and do not allow cells to reach 100% confluence... -
AAV Production in HEK293 Cells
TypeProtocol...cells. Pass the cells twice a week during the maintenance phase and do not allow cells to reach 100% confluence... -
Typing CRISPR Systems
TypeBlog Post...https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K. S., Demircioglu... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post... happens due to a process called Fluorescence Resonance Energy Transfer (FRET). Figure 1: Emission... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post... the annealed cr:tracrRNA is then mixed with recombinant Cas9 protein to form the ribonucleoprotein complex... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...mouse line itself. Generation, breeding, and maintenance of a mouse line is also an expensive and time-consuming... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...Rajakumar, P. D., Prekop, H.-T., Chew, Y. T., Manivannan, S. N., & Chew, W. L. (2021). Programmable C:... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...management, alliance management, business development, finance, and operations. To learn more, visit www.propelcareers.com... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post... . https://doi.org/10.1021/ja512664v Jain PK, Ramanan V, Schepers AG, Dalvie NS, Panda A, Fleming HE,... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post... K., Feliciano, P. R., Arif, A., Bohnuud, T., Fernandez, T. P., Gehrke, J. M., Grayson, P., Lee, K. D.... -
Adenovirus Guide
TypeGuide...in a higher background of non-recombinant adenoviral plasmids. Recombinant adenoviral plasmids are then...vectors for recombinant adenoviral vector production that do not contain GFP. Recombinant adenoviral vector...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type adenoviruses have...have been modified by researchers to create recombinant adenoviral (rAdV) vectors in order to deliver genetic...recombination have not occurred. Once verified, the recombinant plasmid is linearized with PacI to create a linear.... Common Uses of Adenoviral Vectors Although recombinant adeno-associated viral (rAAV) vectors are often... -
Adenovirus Guide
TypeGuide...in a higher background of non-recombinant adenoviral plasmids. Recombinant adenoviral plasmids are then...vectors for recombinant adenoviral vector production that do not contain GFP. Recombinant adenoviral vector...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type adenoviruses have...have been modified by researchers to create recombinant adenoviral (rAdV) vectors in order to deliver genetic...recombination have not occurred. Once verified, the recombinant plasmid is linearized with PacI to create a linear.... Common Uses of Adenoviral Vectors Although recombinant adeno-associated viral (rAAV) vectors are often... -
Antibody Guide
TypeGuide...Antibodies produced this way are called recombinant antibodies. Recombinant antibodies are created by cloning... time and labor intensive. Recombinant expression validation A recombinant expression strategy is used...cultured immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals... of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced from plasmids...abundant isotype in blood, also found in tissues; predominant isotype in secondary immune response Monomer ...Single-domain antibodies (sdAbs), also called nanobodies, are ~12-15 kD single heavy domain fragments ...Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared... -
Antibody Guide
TypeGuide...Antibodies produced this way are called recombinant antibodies. Recombinant antibodies are created by cloning... time and labor intensive. Recombinant expression validation A recombinant expression strategy is used...cultured immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals... of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced from plasmids...abundant isotype in blood, also found in tissues; predominant isotype in secondary immune response Monomer ...Single-domain antibodies (sdAbs), also called nanobodies, are ~12-15 kD single heavy domain fragments ...Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared... -
Adeno-associated virus (AAV) Guide
TypeGuide...Guide . Recombinant AAV Vectors Wild-type AAVs have been modified by researchers to create recombinant adeno-associated...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...in order to deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the ...Created with BioRender.com. Genome Integration Recombinant AAVs are generally considered to not integrate...cystic fibrosis and cancer. Neuronal Tracing Recombinant AAV vectors have been widely used by neuroscientists...P. J., Mandel, R. J., & Muzyczka, N. (2004). Recombinant AAV viral vectors pseudotyped with viral capsids...sites in AAV virions during AAV wild-type and recombinant AAV vector production . Journal of Virology, ... -
Adeno-associated virus (AAV) Guide
TypeGuide...Guide . Recombinant AAV Vectors Wild-type AAVs have been modified by researchers to create recombinant adeno-associated...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...in order to deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the ...Created with BioRender.com. Genome Integration Recombinant AAVs are generally considered to not integrate...cystic fibrosis and cancer. Neuronal Tracing Recombinant AAV vectors have been widely used by neuroscientists...P. J., Mandel, R. J., & Muzyczka, N. (2004). Recombinant AAV viral vectors pseudotyped with viral capsids...sites in AAV virions during AAV wild-type and recombinant AAV vector production . Journal of Virology, ...