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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 393 results for: prep

Showing: 381 - 393 of 393 results

  1. General Transfection

    Type
    Protocol
    ... (PES) filter Syringes for filtering Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM L-alanyl-...empirically determined for each new batch of 1 mg/mL PEI prepared. There may be variation between batches of PEI...empirically determined. Once a batch of PEI is prepared, transfect cells with a fluorescent plasmid using...

  2. Immunocytochemistry

    Type
    Protocol
    ...primary antibodies raised in a mouse. Reagent Preparation Permeabilization buffer: Dilute 20 µL of Triton...100 in 50 mL PBS. 300 nM DAPI working solution: Prepare a 300 µM DAPI stock solution by diluting 2.1 µL...DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution by diluting 5 µL...

  3. Lentivirus Production

    Type
    Protocol
    ...plasmid encoding your insert of interest Reagent Preparation DMEM Complete (also known as D10): 10% v/v FBS...Incubate the cells at 37 °C, 5% CO 2 for ~20 h. Prepare a mixture of the three transfection plasmids into...empirically determined. Once a batch of PEI is prepared, transfect cells with a fluorescent plasmid using...

  4. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...their resistance genes, as one does not need to prepare separate batches of antibiotic-containing agar....or other antibiotic resistance) Procedure Day 1 Prepare carbenicillin to a concentration of 1 mg/mL – 4...

  5. Science Guides

    Type
    Guide
    ...Pooled Libraries Pooled libraries are single preparations of many different plasmids. Plasmids within ...

  7. Chemogenetics Guide

    Type
    Guide
    ...available. Browse our collection of Ready-to-use AAV Preparations of Chemogenetic Plasmids ! Table 4. Common promoters...

  8. Centrifugation

    Type
    Protocol
    ...many different experimental procedures, such as minipreps and RNA extractions. Plus, centrifuges are useful...

  10. Using a Light Microscope Protocol

    Type
    Protocol
    ...image of that object! Equipment Light Microscope Prepared Slides (or other sample that can fit on a microscope...

  13. Kit Free RNA Extraction

    Type
    Protocol
    ...Protocol Before starting this protocol, make sure to prepare solution D (see reagent section above for the recipe...
Showing: 381 - 393 of 393 results