We narrowed to 482 results for: vent

1. Hot Plasmids and Viral Preps - May 2021

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   ...INTRSECT viral preps available! We are expanding our inventory of ready to use viral preps from the Deisseroth...

2. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   ...through two sequential homologous recombination events: The plasmid carrying the desired genetic modification...

3. Addgene's Cancer Collection Pages Connect the Research Community

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   ...of cancer, however, the apoptotic pathway is circumvented because of the specific genes involved. Downregulation...

4. Plasmids 101: Optimizing Plasmid Yields

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   ...optimal growth, as insufficient gas exchange will prevent your cultures from reaching the desired density...

5. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...bioeconomy, and that it’s been 40 years since the invention of genetic engineering. What's incredible is the...

6. MXS Chaining

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   ...: PMC2574415. 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic assembly of...

7. Tips for arabidopsis transformation

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   ...resistant plants will be affected by the selection eventually. Genotype putative T1 transformants: When your...

8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...tethered to a repressive domain, such as KRAB, to prevent efficient transcription from sgRNA targets. CRISPRi...

9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   ...recently been described in vitro, with the hope of eventually using this method for in vivo applications. This...

10. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...inhibitors are tested in parallel for their ability to prevent BiFC resulting from the assayed PPI. This high-...

11. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ...the dilemma of creating a technology that could eventually replace scientists themselves.  The sections ...

12. Negative Can Be Positive: Open AAV Data with Addgene

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    ...recombinant AAV2 particle aggregation and methods to prevent its occurrence during vector purification and formulation...

13. Summer SciComm Series: Modes of Communication

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    ...accessible via web searches; likewise, most in-person events and venues will have a solid understanding of their...

14. An Introduction to Adenovirus

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    ...intracellularly-exposed proteins and downstream cellular events, all recruit strong innate and adaptive immune ...

15. CRISPR Antimicrobials

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    ... both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance genes...

16. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...arguing against this approach. First, this would prevent the study of the proteins independently from each...

17. Tips for Screening with Yeast Two Hybrid Systems

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    ... proteins must gain access to the nucleus. To circumvent this requirement for transmembrane proteins, ...

18. Plasmids 101: Protein tags

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    ...fusion proteins to your protein of interest would eventually get ridiculous (you generally don’t want more...

19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...technologies may be very effective in transforming conventional research. So, I hope my younger colleagues may...

20. Your Lentiviral Plasmid FAQs Answered

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    ...the 3'LTR in the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target...