...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive...none Cre dependent 1, 5, 9, rg* GENIE 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m none Cre-dependent...none Cre dependent 1, rg*, PHPeB Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG jGCaMP7s none Cre dependent... none Cre dependent 1, 9 Kim , GENIE 100839 pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 CAG GCaMP6m none Cre dependent...none Cre and Flp dependent 8 Deisseroth 137120 pAAV-Ef1a-Con/Foff 2.0-GCaMP6M EF1a GCaMP6m none Cre dependent...

...the tables below for CRISPR prime editing plasmids. Plasmids are available for expression in mammalian...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert...selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...prime editor plasmids for (search and replace) genome editing. CRISPR... CRISPR Prime Edit CRISPR Plasmids: Prime Edit...

...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...locus and encodes a primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In...Ampicillin none, need Cas9 plasmid Qi Do you have suggestions for other plasmids that should be added to ...replaced by guanosine to create A->G (or T->C on the opposite strand) mutations. ID Plasmid Promoter PI Publication...

...systems Packaging Plasmid All on one plasmid: Gag, Pol, Rev, Tat Two plasmids: one encoding Gag and Pol and...integration. Packaging plasmid(s) Envelope plasmid Addgene’s packaging and envelope plasmids are generalized ...single packaging plasmid encoding the Gag, Pol, Rev, and Tat genes. The transfer plasmid contains the viral...packaging system is split into two plasmids: one encoding Rev and one encoding Gag and Pol. Although safer, ...and another encoding Rev Envelope Plasmid Interchangeable: usually encodes for VSV-G Interchangeable: ...usually encodes for VSV-G Safety Safe. Replication incompetent: Uses 3 separate plasmids encoding various... 3-4 plasmids are transfected into A293T cells: one transfer plasmid one or two packaging plasmid(s) one...

...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function...locus and encodes a primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...variety of Cas-containing plasmids. Do you have suggestions for other plasmids that should be added to ...This technique creates diverse populations of mutants for directed evolution. ID Plasmid Gene/Insert Promoter...

... Vectors Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...studies Addgene has a collection of plasmids suitable for creating individual fluorescently tagged proteins...Addgene's collection of plasmids suitable for FRET experiments to measure protein interactions or conformation... Plasmid Collections Fluorescent Proteins FRET Fluorescent...of interest and the listed fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial... to Top FRET Reference Standards The following plasmids have well-characterized FRET efficiency values...

...locus and encodes a primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...paired with a wide variety of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...replaced by guanosine to create A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter...expression plasmids that have been designed for use in plants. CRISPR...

...with the post-design process such as primer and plasmid design. Examples:  “multiplex design”, “multi-method...dump of ALL strings in a genome using any IUPAC encoded pattern at any edit distance. It won’t, however...is a significant hurdle made worse by an ever increasing marketplace. Since 2013 about 11 tools have been...the user is expected to interact with the tool (a screen shot and overview of the table can be found above...re engaged in heavy CRISPR design (I’m talking screens and/or very large genomes), then it is worthwhile...

...Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections Background...interest tTA expression plasmid Components for a tetracycline on system are: Plasmid containing a tetracycline-dependent...generation lentiviral plasmid for inducible expression of shRNA; neomycin selection; plasmid 21915 has puromycin...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Tetracycline (Tet) Inducible Expression...Tetracycline Off Tetracycline On Experimental Tips Tet Plasmids Background To advance the study of gene function...uptake. Search our collection for all tetracycline plasmids. Tet System and the Tet Response Element (TRE)...

... are not encoded by the transfer plasmid, but instead are provided in trans by other plasmids or the packaging...components are needed: γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: ...γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, however... trans by specific plasmids. This method, therefore, reduces the number of plasmids that are required ...γ-retroviruses self-inactivating (SIN)? They can be. Plasmids that encode SIN γ-retrovirus lack most of the U3 region...do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to...selection. Return to Top Glossary Plasmid Type Element On Same Plasmid as Transgene? Purpose Envelope VSVG...