We narrowed to 482 results for: vent

1. CRISPR Activation: A Practical Guide

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   ...activated. However, there are several factors that may prevent your gene from being up-regulated. For instance...

2. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   ...and other Cas proteins like SaCas9 and Cpf1 can circumvent the limitations associated with SpCas9. Cas13a...

3. Plasmids 101: Gateway Cloning

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   ...resources, time, and experimental needs. Since its invention in the late 1990s, Gateway cloning technology ...

4. Save Time with Transient Plant Leaf Transformations

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   ...species This will save you a lot of time. Don't re-invent the wheel. Test different leaf ages Usually younger...

5. New Tools Enable CRISPRa for Neuroscience Applications

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   ...adeno-associated vectors (AAV). This issue was recently circumvented by the Yang Lab with the development of a Suntag-p65...

6. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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   ...resemble spaghetti. We have two plasmids in our inventory from the lab of Eric Wieschaus that express sqh...

7. Supporting Reproducibility with a Connected ELN

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   ... the Wisconsin research community were to: (1) prevent vendor lock in by making it easy to get data out...

8. CRISPR-mediated Plant Base Editors

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   ... (Komor et al., 2016 and Nishida et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase...

9. To Codon Optimize or Not: That is the Question

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   ...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself.  Caveats ...

10. Hot Plasmids - August 2020

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    ...Michelle Cronin The benefits of nanobodies over conventional antibodies can’t be overlooked. Nanobodies are...

11. An Inside Look into Addgene's Dev Team

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    ...writing code for common tasks, it would be like re-inventing the wheel over and over again for every single...

12. Neuronal labeling with Spaghetti Monster

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    ...microscopy As indicated above, smFPs can be used with conventional microscopes, such as epifluorescence or confocal...

13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...significantly updated in 2022 by Susanna Stroik.  The advent of CRISPR/Cas9 has made it easier than ever to ...

14. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    ...systemically (retro-orbitally, RO; or intracerebroventricularly, ICV) or locally (stereotaxically, STX...

15. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ...plasmid map and identify any mismatches that are preventing your overhangs from aligning properly. Do this...

16. Twenty Years of Sharing: Expanding Accessibility

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    ...grouping similar tools together in what would eventually become our collection pages. These pages allowed...

17. Hot Plasmids - November 2023

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    ...showed they could record transcriptional activation events as bands of induced GFP-iPAK4 expressed under promoters...

18. Viral Production at Addgene

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    ... had a case of mycoplasma contamination. In the event of contamination, all of the virus produced in the...

19. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...by 2019 (Figure 5A). We then added jGCaMP7 and eventually jGCaMP8, later generations of GCaMP6, to the ...

20. Antibodies 101: The Four ELISAs and When to Use Them

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    ...set up. Then they block the capture reagent to prevent non-specific interactions. Next comes the binding...