We narrowed to 474 results for: organization Addgene

...SynBio Genome Editing Gene Silencing Reporters Addgene’s repository contains many plasmid tools that were...other species. In addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections...engineering and gene expression to reporter systems. Addgene’s collection includes plasmids containing promoters...plasmids for gene editing in plants. Please refer to Addgene's Plant CRISPR Resources for a full selection of...truncatula . Return to top Other Plant Resources Addgene Blog Blog Posts on Plant Biology RUBY-Red Siliques...arbuscular mycorrhiza-inducible promoters to label organelles, membranes and cytoskeletal elements in M. truncatula...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Addgene Depositors that have received funding from the Neuroendocrine Tumor Research Foundation...Neuroendocrine Tumors Using Adult Stem Cell-Derived Organoids Matthew Meyerson Dana-Farber Cancer Institute ...

...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...intensive. Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...

... autophagy biosensors currently available from Addgene. Rosella autophagy biosensor Rosella is a dual-...-targeted and mitochondria-targeted Rosella at Addgene! Keima autophagy biosensor Keima is a mammalian...Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy... red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that releases... flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana Maria Cuervo...PMCID: PMC5618514. Additional resources on the Addgene blog Beware of fluorescent protein oligomerization...process by which cytoplasmic material, including organelles, are targeted to lysosomes for degradation. Autophagy...

...fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system was developed...mitochondria-targeted variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou...PubMed PMID: 19185711. Additional Resources on the Addgene Blog Beware of Fluorescent Protein Oligomerization...monitoring and analyzing autophagy of cytosol and organelles in yeast cells. Autophagy (Greek for “self-eating...by a lack of nutrients and targets cytosol and organelles to the vacuole/lysosome for degradation and recycling...more fine-grained detail about what proteins or organelles are being degraded) and it is slow. Alkaline...for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy,4(2), 205-213. doi:10.4161...

...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...Plasmid Tables Additional Resources Mammalian organisms are exposed to millions of potential pathogens...presenting cells. B cells in secondary lymphoid organs recognize specific antigens through the B cell ... 25 cellular signaling pathways and processes. Addgene Blogs Adeno Associated Virus (AAV) for Cell and...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular Posts of 2014 ...year in science – as seen through the lens of Addgene’s blog and plasmid repository. Our blog is just ...Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making Your Own Competent...101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick...

...standard FPs. The moxFPs are now available through Addgene Many thanks to our guest bloggers Erik L. Snapp...Central PMCID: PMC2150754. Resources Avaliable at Addgene Read Our Blog Post on Protein Tags Read Other ...Protein Pages Find the mOX Fluorescent Proteins at Addgene   ...distinct subcellular environments, including the organelles that compose the secretory pathway, endocytic...formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible to these non-native modifications... Lee Snapp. "Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and...

...Contacts for Your Dream Job Resources on Addgene.org Addgene Careers Page ...perfect application. Additional Resources on the Addgene Blog Learn How to Experiment with New Careers ...that requires travel can show that you are self organised, reliable, and can work independently. Practical...involves traveling, showing that you are a highly organised and experienced traveler by citing specific examples...

... 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide AAV Viral Preps References  Chen...Resources and references More resources on the Addgene blog Viral Vectors 101: Viral Vector Elements Viral... Vectors. Microorganisms, 12(2), 310. https://doi.org/10.3390/microorganisms1202031038399714. PMID: 38399714...

...CRISPR History and Development Resources on the Addgene blog CRISPR Antimicrobials Treating Muscular ...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ... post was originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik.  References...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...1563. doi: 10.3390/ijms24021563 Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook...

...PubMed PMID:18647110. Additional Resources on the Addgene Blog Read More about Fluorescent Protein Imaging...are better for whole cell imaging and studying organelle trafficking. Monomeric PA-FPs are usually preferred...involve enhancing optical live imaging of proteins, organelles, and cells. Similar to FPs, PA-FPs allow for ...localization, turnover, and trafficking, as well as organelle trafficking and dynamics (fission, fusion, etc...mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during...Catch Up on Your GFP Background Resources at Addgene.org Find Photoactivatable FPs in Empty Backbones...

...we will not delete @addgene on X. This will additionally ensure that the @addgene handle is always associated...media, you can find us on Bluesky: @addgene.bsky.social; or @addgene on Instagram, TikTok, Facebook, and...an official Addgene account. You can reach out to us directly via email at help@addgene.org, and we invite...Addgene is announcing we have chosen to stop all activity on X, formerly Twitter. If you are interested...mainstay of conversation in the scientific community. Addgene has been considering a gradual move off X as a ...to paid content regardless of user preference. Addgene is a values-driven nonprofit. Recent remarks and...posting, monitoring, and resharing content on X.  Addgene deeply values our community and recognizes the ...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... Today on the Addgene Podcast, we have another interview conducted by European Outreach Scientist Benoit...Additional Resources from the Addgene Blog Visit our Fluorescent Proteins featured topic page Read Joachim's... addgene podcast...Visualizing Subcellular Structures and Organelles Resources at Addgene.org Find Fluorescent Protein Plasmids...

...cover it. Addgene: OK, thanks for your time, and thanks for depositing your plasmids with Addgene. Howarth... and its potential uses. Developing a new tool Addgene: Where did you get the idea to develop SpyLigase...just join one peptide tag to another peptide tag. Addgene: What is the advantage of peptide tags for building...bonds, so there were no weak links in the chain.  Addgene: What about the Streptococcus pyogenes protein ...pili that they use to anchor on to host cells. Addgene: Were there any major hurdles along the way? Howarth...capture system.) Cancer and other applications Addgene: What does this technology mean in the context ...capture or for capture using microfluidic devices. Addgene: What other uses do you foresee for this tool? ...

...Developing the Addgene microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists...materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read Our ...The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see the ...with lots of researchers who weren’t aware of Addgene’s plasmid sharing services, as well as #happydepositors...dedicated to science communication through art. Addgene Advisory Board member Siouxsie Wiles gave us a ... about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence...creating well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular screening...

...two-color pair. We'll be sending it to Addgene in a matter of days. Addgene: Can you tell us a little more about..., or other conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of...available. Visit Ed Boyden lab's Addgene page and click the link in the Addgene Alerts box. References: ...activity of two populations of neurons at once. Addgene spoke to Boyden about this latest optogenetics ...missing. Optogenetic tools for neuroengineering Addgene: For those who may not be experts, can you give...Understanding the brain through optogenetics research Addgene: What are some of the big problems that optogenetic...these tools, so we can focus on the next round. Addgene: What tools are still missing from the optogenetics...

...with detailed protocols, go to www.addgene.org/crispr/tagging. Addgene: What motivated you to adapt CRISPR...tagging system, which they’ve now made available at Addgene. For more details on the plasmids that are currently...can now look at many more transcription factors. Addgene: How does the system work? Mendenhall: The system...antibodies. The results look almost identical. Addgene: What advantages does your system have in this ...faster. It’s also easy to run things in parallel. Addgene: Besides being fast and efficient, what other challenges... hundred different versions of this out there. Addgene: Why was it important to you to make these plasmids...waiting and hands on labor. That’s a huge deal. With Addgene, now these can be available to anyone for a modest...

...PMCID: PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation...there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might...microscope, fractionate samples to isolate particular organelles and their contents, or perform in situ hybridization...Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All Fluorescent...

...communication blog posts Resources on Addgene.org Find careers at Addgene ... project I’d seen in my already long tenure at Addgene. I would work with our research team and software...pinpointed and (mostly) solved two main problems with Addgene’s cross team communication. Although these real ...these plates now” to a fellow team member. But, at Addgene, we use seven different types of “plates” (Fig....not the container was barcoded (this is pretty Addgene specific) The types or purposes of each given container...that there were a lot of words that nearly all Addgene teams used that seemed simple, but they could in...solution for this problem was the creation of an Addgene lexicon. The base lexicon was and continues to ...