We narrowed to 517 results for: pes
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TypeBlog Post...than one theme and can be used to answer multiple types of questions. Writing a rough outline of these stories...
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Plasmids 101: Modular Cloning
TypeBlog Post...we’ll cover the basics of what MoClo is and what types of experiments it enables. What is modular cloning... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...other Cas variants described, nearly all genomic landscapes should be editable with CRISPR technology. ... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...Zhang Lab 2015) Reference Feng, S., Sekine, S., Pessino, V. et al. Improved split fluorescent proteins ... -
A Look at Addgene's QC Process
TypeBlog Post...worldwide. Depositing scientists provide different types of data for each of their plasmids, from descriptions... -
CRISPR in the Clinic
TypeBlog Post...progress in clinical trials. Figure 1: Types of delivery and editing strategies for CRISPR therapies... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post...Golden Gate Assembly worked for all the assembly types, circular assemblies yielded 500 times the number... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...mixture of antibodies recognizing many different epitopes. (Middle) Monoclonal antibodies produced from ... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post... due to their low immunogenicity and range of serotypes allowing preferential infection of certain tissues... -
Plasmids 101: FLEx Vectors
TypeBlog Post...switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping. PubMed... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post... monitor and track them. We have a number of microscopes, including an automated microscope but we need... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...fairly flexible and adaptable to cloning different types of DNA, such as cDNA, PCR fragments, or genomic ... -
10 Basic tips for mammalian cell culture
TypeBlog Post...people use 70% ethanol, but you can also use Incidin wipes) before and after your work. Keep your contaminants... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post... solution (diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature ... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...light and, practically speaking, for common lab microscopes the detection limit is 200 nm. The size of most... -
Tips to Make the Most of a Scientific Conference
TypeBlog Post... be sure to practice beforehand. Generally, two types of people will visit your poster: those that want... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...streamlined suite of tools for using CRISPRi in many types of bacteria: lab-adapted, environmental isolated... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...gene disruption frequencies in the various cell types ranged from 18% to 65% (Maggio et al., 2014). An... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...off of plasmids in bacteria, and they can target epitopes that are hard for antibodies to get to, like ones... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...again see quick adaptation and steady use of all types of libraries, once made available through the repository...