We narrowed to 588 results for: ret

1. New and Upcoming Viral Vectors - September 2019

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   Blog Post

   ...aliquots in AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde. You can also request the specific serotypes ...

2. New and Upcoming Viral Vectors - June 2019

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   Blog Post

   ...these tools both for pilot experiments and for interpreting the results of studies. When planning AAV experiments...

3. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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   Blog Post

   ...“These novel methods will be instrumental in interpreting human variation at large scales and making personalized...

4. Viral Vectors 101: Inverted Terminal Repeats

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   Blog Post

   ...transgene is flanked by two ITRs, and the ITRs are retained with the gene in a recombinant AAV (rAAV) vector...

5. "Build Your Plasmid": The Game - Play to Teach

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   ...YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies...

6. Visualizing Translation at the Single Molecule Level

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   Blog Post

   ... nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind cognate untranslated mRNAs...

7. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

   ...highly optimized bioinformatics pipeline that will return high-quality assembled and circularized plasmids...

8. With Our New Viral Service, We're Taking CRISPR Further!

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   Blog Post

   ...next-generation sequencing to ensure that the library has retained gRNA representation, produce high titer lentivirus...

9. R Bodies: Membrane-Rupturing Microscopic Tools

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   ...force-generating protein machines that can extend and retract over many microns in response to pH changes without...

10. Components of CRISPR/Cas9

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    Blog Post

    ...repeated elements “direct repeats” and the unique stretches of DNA between them “spacers” (see figure below...

11. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post

    ...proteins, imaging four colors simultaneously should be pretty straightforward. Thank You to our Guest Blogger...

12. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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    Blog Post

    ...dye! Always run a ladder. Ladders allow you to interpret the bands that you get in your sample lanes. Choose...

13. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post

    ...nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes interact with ...

14. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

    ...CRISPR arrays into guide RNAs. crRNA cleavage is retained in dCas13d and is thus HEPN-independent. These...

15. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ...incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs (right ...

16. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post

    ...instead of incorporation via plasmid or media. Theoretically, if you put all these together, the cell will...

17. Plasmids 101: Screens vs. Selections

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    Blog Post

    ...entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically not feasible...

18. Targeted Mutagenesis with EvolvR

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    Blog Post

    ... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...

19. Antibodies 101: Isotypes

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    Blog Post

    ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

20. Antibodies 101: Monoclonal Antibodies

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    ...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...