We narrowed to 482 results for: vent

1. CRISPR Activation: A Practical Guide

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   ...activated. However, there are several factors that may prevent your gene from being up-regulated. For instance...

2. Plasmids 101: E. coli Strains for Protein Expression

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   ... problem for toxic protein products, which can prevent the culture from reaching the desired density within...

3. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   ...requires H2O2 which can be toxic to living cells. To circumvent these issues, the Ting lab utilized yeast display-based...

4. The Fluorescent Vegetables in Aptamer Soup

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   ...molecules to induce their fluorescence. Until a binding event occurs, neither the aptamer nor the small molecule...

5. Plasmids 101: Biotinylation

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   ...unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin is...

6. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   ...deficient in dUTPase and uracil deglycosidase. dUTPase prevents the buildup of dUTP and uracil deglycosidase removes...

7. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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   ...means to manage their work, combining data, team, inventory and project management functionalities with experiment...

8. Adeno-associated Viruses (AAVs) for Genome Editing

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   ...trap enrichment, whereas pAAV-TK-Acceptor has a conventional heterologous promoter-driven TK-neoR gene. To...

9. Quick Guide to Near-Infrared Fluorescent Proteins

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   ...biosensors NIR reporters can tell us about cellular events via  fluorescence changes occurring due to protein...

10. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...2015) Vectored antibody gene delivery for the prevention or treatment of HIV infection. Current Opinion...

11. Filming Science Videos in the Age of Social Distancing

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    ...control, and extra hand sanitizer.    Cycle 4: Venturing into the Lab Design: The lab is the beating heart...

12. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    ... fusion protein away from its usual location, preventing it from carrying out its proper function (Figure...

13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...integration, and how researchers have modified them to prevent this integration.  Lentiviruses evolved to integrate...

14. Hot Plasmids: Summer 2024

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    ...enrichment and structure determination By Mike Lacy Conventional cryogenic electron microscopy (cryo-EM) protocols...

15. Antibodies 101: Beyond Surface Labeling

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    ...surface of the CD8+ T cells and gate on the positive events for your flow analysis. However, certain downstream...

16. Evolution of Lab Techniques

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    ...real-time PCR Southern blotting, named for its inventor Edward M. Southern (1), can be used to detect ...

17. Plasmids 101: Common Lab E. coli Strains

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    ... K-12 was isolated from a patient in 1920 and eventually led to the common lab strains MG1655 and its ...

18. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...epigenetics As with many TALEN-based technologies, the advent of CRISPR has made targeting much easier! With ...

19. A Control for All Seasons

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    ...an alternative but well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples...

20. PiggyBac-ing Through the Genome Editing Field

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    ...disrupt the target site for the nuclease thereby preventing re-cutting. Transposase removal of the cassette...