We narrowed to 542 results for: SHI

1. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

   ...photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation) domains on either side...

2. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   Blog Post

   ... experience that is gathered can be shared by publishing it as (part of) figure 1, uploading it as a preprint...

3. Choosing the B(right)est Fluorescent Protein: Photostability

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   ...does not depend on excitation power in a linear fashion. This implies that reducing the power 2-fold does...

4. Viral Vectors 101: An Introduction to AAV

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   Blog Post

   ...Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed PMID: 26427452. Issa, ...

5. The time and cost required to make a plasmid

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   Blog Post

   ...things go wrong, replace them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up...

6. Plasmids 101: The protein expression toolbox

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   Blog Post

   ...and a custom gRNA is all you need to generate a frameshift mutation that will permanently ablate protein...

7. CRISPR 101: Cas9 vs. The Other Cas(s)

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   Blog Post

   ...genome engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes...

8. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...screening method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage...

9. Antibodies 101: Selecting the Right Antibody

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   Blog Post

   ...feel free to make the decision by price point, shipping time, or other factors. Just remember that these...

10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ...Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory...

11. CRISPRainbow and Genome Visualization

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    Blog Post

    ...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...

12. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ... and reverse reactions, respectively, and then shipped along with their corresponding glycerol plate on...

13. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    ...Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript by Maaike Pols, PhD, ...

14. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post

    .... The Apollo 11 mission circuitry that took a spaceship to the moon was built on NOR gates. It’s a fundamental...

15. Working with Nuclear Receptors

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    Blog Post

    ...coregulators. We look forward to growing this important relationship with Addgene for the benefit of the entire research...

16. Optogenetics Guide

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    Guide

    ...PsChR1 Red-shifted chloride-conducting channel from Proteomonas sulcata 540 Phobos Blue-shifted iC++ variant...467 Aurora Red-shifted anion-selective ReaChR variant 517 Halorhodopsins Jaws Red-shifted, light-driven...kinetics: Examples: ChETA, C1V1(t/t), ChrimsonR Red-shifted peak action spectra Examples: VChR1, C1V1(t/t),...amplitude Examples: eNpHR, eNpHR2.0, eNpHR3.0 Red-shifted peak action spectra Example: Jaws Browse Halorhodopsin...K176R mutation. 590 PsChR2 High-efficiency blue-shifted channelrhodopsin from Platymonas subcordiformis...2014. Noninvasive optical inhibition with a red-shifted microbial rhodopsin. Nat Neurosci. 17(8):1123-9... Yizhar O, Hegemann P, Deisseroth K. 2008. Red-shifted optogenetic excitation: a tool for fast neural ...

17. Antibodies 101: Stripping and Reprobing Western Blots

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    ...Note that βME reacts with antibodies, so thorough washing is critical. Assess the stripping Now it’s time...

18. Plasmids 101: Modular Cloning

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    Blog Post

    ...identify the best cocktail of genes when the relationship between genotype and phenotype is best described...

19. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    .... PubMed Central PMCID: PMC18018. 2. Miyawaki, Atsushi, et al. "Fluorescent indicators for Ca2+ based ...

20. Hot Plasmids and Viral Preps - January 2021

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    Blog Post

    ...AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical...