...leading a professional development workshop. Specifically, Jo, Brook, and Angela are co-leading "Making...

...open platform for scientists using AAV to share practical information about their experiences. It will contain...

...a technology is so useful it forever enhances biological research and discovery. CRISPR is a great example...

...Rob Hurt, whose expertise and suggestions were critical to the development and writing of two blog posts...

...demethylation. AAV Q&A with Tim Miles: While not technically a research story, I couldn't resist adding in...

...on Verifying Your Plasmid Table 1: Comparing Typical Comericial Ladders with the PSU Ladders DNA ...

...Addgene.  Learn how discoveries were made and get practical tips on how to use the plasmids in your own lab...

...with Addgene Board Member, depositor, and Harvard Medical School researcher Connie Cepko. Listen to the podcast...

...February 13, 2021 Westenberg, a Professor of Biological Sciences at Missouri University of Science and...

...Bassik's Lab - A new tool for making diverse, localized point mutations. Listen to Our Segment on CRISPR...

... plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to create...

...Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite you to head...

...Mark your calendars for November 16-20, 2020 for #DepositorWeek on Twitter!  That’s today through Friday...

...Plasmids for FRET Find Plasmids for Subcellular Localization ...

...Once your request is received, we’ll review for technical and legal compliance, and then send you an email...

...learned a lot about how FRET can be used to study biological phenomena and about how fluorophores other than...

...empower those that need to face these challenges locally. We look forward to more collaborations with Seeding...

... any tags or fusions that are on your insert. Typically you would list any tag or fusion protein in the...

...project and constructing ever more complicated biological devices. Chapter 6: - Making research go faster...

...02139 Have a question? Email: [email protected] Call: +1 (617) 225-9000         ...

... global coffee supply… My back-of-the-napkin calculations put the daily consumption here at ~ 60,000 cups...

...   References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout screening in human cells." ...

... your DNA sequence Finally, when you do see a miscalled peak, don’t be shy. Feel free to edit it. Most...

...disorders are frequently overshadowed by other medical conditions, so these topics have become a cornerstone...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...

...about how my ideas would be translated through Zoom calls and Google docs. I pondered on the ways that I wouldn't...

...Joanne Kamens, responded by starting something we call “Random Lunches.” Once or twice a year, she’ll mix...

...approach." Proceedings of the Royal Society B: Biological Sciences 272.1577 (2005): 2117-2126. PubMed PMID...

...the carats in the datatable headers to sort alphabetically or in ascending or descending order, in the...

...accomplishments, research/professional goals and feedback. Calendar: Month-by-month look at the next year, including...

.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...

...transfer plasmid sequences into the host genome. Typically it is the sequences between and including the ...whereas standard retroviruses can only infect mitotically active cell types. This means that lentiviruses...Lentiviral transfer plasmids are not designed specifically for transient transfections. Therefore, there...and cloned into the lentiviral vector. This is basically shuffling restriction sites between vectors until... entry vector at a region flanked by sequences (called attP1 and attP2) that allow the insert to recombine...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...

...benefited from the business rigor and efficiencies typically adopted by for-profit companies, while making ...

...exploration in this area will accelerate the launch of clinical applications. During the panel discussion, Dr...

...easier to ship back. Be that person! If you use serological pipettes from Corning®, Falcon®, or Axygen®, ...

...even changes the DNA sequence through a mechanism called depurination. In depurination, the hydrolysis of...

...sessions and reinforced our newfound knowledge with practical understanding of how to apply it. The personal...

...Runne-Janczy’s first day at eScience Labs was not your typical first day. “I walked in and they asked me, 'How...

... when she signed up for a 10 week class at Iowa called “Teaching Your Research.” This course has participants...