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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 472 results for: Sele

Showing: 441 - 460 of 472 results

  1. Ligation Independent Cloning

    Type
    Protocol
    ...the reaction mixture, and may provide counter-selection for negative clones as in the example shown here...removing the stuffer sequence and sacB counter-selection marker (see our restriction digest protocol )....

  2. Genetic Code Expansion

    Type
    Collection
    ... tRNA. Applications By making small changes in selected amino acids within a protein, any alterations ...

  3. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...sequence to be amplified (usually 18-21bp). When selecting restriction sites, you should use a DNA analysis... longer the expected PCR product is. You should select an annealing temperature based on the melting temperature...

  4. Brain Initiative Collection

    Type
    Collection
    ...scientific research. Addgene's AAV are made from a selected set of plasmids that are currently in our repository...

  5. Kit Free RNA Extraction

    Type
    Protocol
    ...for 1 hour. Lithium Chloride (Option B): LiCl selectively precipitates RNA versus DNA or proteins. Add ...

  6. Protocol - How to Design Primers

    Type
    Protocol
    ...chemically by joining nucleotides together. One must selectively block and unblock repeatedly the reactive groups...

  7. Centrifugation

    Type
    Protocol
    ...Learn about selecting and using a centrifuge to separate different components in a liquid sample....

  8. Fluorescence Titering Assay

    Type
    Protocol
    ...adapted to a variety of target cell lines and selection markers. Note, because this protocol uses microscopy...

  13. AAV ddPCR Titration

    Type
    Protocol
    ...target types. When the plate layout is complete, select 'Run' to begin the droplet reading. When the droplet...

  14. Lentivirus ddPCR Titration

    Type
    Protocol
    ...target types. When the plate layout is complete, select 'Run' to begin the droplet reading. When the droplet...

  15. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... et al. 2010. A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. BMC Biology...

  17. Guide to Using Pooled Libraries

    Type
    Guide
    ...employ a selection method relevant to the phenotype being studied. Examples of published selection mechanisms...screen: Apply library Apply selection Most cells die or do not pass selection (in the case of a reporter...on a control sample (no selection) In parallel, apply library Apply selection; most cells will survive...identify those cells that survive post-selection. The selective pressure must be strong enough that most...survive the selection mechanism. You’ll infect two sets of cells and subject one set to selection while the...evaluate which library components are influenced by selection, so it’s essential that you have access to this...pass (“win”) Get a list of genes involved in the selection NEGATIVE screen: Apply library Perform Next Generation...

  18. Plan Your Experiment

    Type
    Guide
    ...Experiment Introduction Select Genetic Manipulation Design and Clone gRNA Select Expression and Delivery...expression vectors . Select Your Expression and Delivery System Once you have selected your target and designed... to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering... CRISPR experiment. Created with BioRender.com. Select Your Desired Genetic Manipulation Different genetic...manipulations require different CRISPR components. Selecting the genetic manipulation you plan to perform will... in mind when designing and cloning your gRNAs. Select Genetic Element to be Targeted The exact region...of your protein expressed in your system. HDR : Select a cut site as close as possible to the location...

  19. Molecular Biology Reference

    Type
    Guide
    ...promoters reference page . Selectable Marker The selectable marker is used to select for cells that have successfully... the purpose of replication. The selectable marker enables selection of a population of cells that have...plasmids Antibiotics commonly used for plasmid selection DNA sequencing for plasmid verification Resources...the plasmid within bacteria, while allowing for selection against any bacteria not carrying the plasmid....to use. Antibiotic Resistance Gene Allows for selection of plasmid-containing bacteria by providing a ...and out-populate bacteria with plasmids, thus selecting against the propagation of these plasmids through...ampicillin. Under these conditions, there is a selective pressure to retain the plasmid DNA, despite the...

  20. Addgene's eBook Collection

    Type
    Guide
    ...Addgene's eBooks are here to help! 0 of 0 Selected Download Select Plasmids 101 September 2023 | Fourth...promoters and cloning techniques! Close About Overlay Select CRISPR 101 January 2021 | Third Edition About...libraries in genome-wide screens. Close About Overlay Select Fluorescent Proteins 101 August 2017 | First...fluorophores; and much more! Close About Overlay Select Viral Vectors 101 July 2024 | Second Edition...production and use, and much more! Close About Overlay Select Science Career Guide November 2018 | First ...the job application process. Close About Overlay Select CRISPR 101 en español January 2024 | First ...libro electrónico, CRISPR 101. Close About Overlay Select Antibodies 101 New! October 2024 | First Edition...
Showing: 441 - 460 of 472 results