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...cellular background. All cell lines and phenotypic datasets that result from this effort will be broadly shared...

...mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette for expression of both a “+” and “-” heterodimeric...

...pShuttle-CMV (Addgene #16403) plasmid with Gateway cassette Zhang 50958 ShuttleNSE For production of viruses...

... function, providing a reliable and accessible toolset for scientists worldwide. ID Recombinant Antibody...

...plasmids from different combinations of a common set of components (Weber et al., 2011). This method, ...tools, and more. The MoClo system consists of three sets of cloning vectors (Level 0, 1, and 2) which can...Bacterial Expression Thorsten Mascher , Georg Fritz Two sets of plasmids to generate constructs for Lactiplantibacillus...create plant expression vectors containing several cassettes and generate multi-construct transgenic plants...protein complexes in various hosts. Expression cassettes for individual subunits are first assembled by...

... following intravitreal injection in mice and marmosets as compared to the AAV2 serotype. The AAV2(Y444F...

... opens in a new window) COVID-19 Open Research Dataset (CORD-19) (Link opens in a new window) LitCovid...

... 21179091), as well as (v) distinct selection cassettes on pTAL7a and pTAL7b for enrichment of double-...

... and 18 months of age. A defining feature, with onset typically between 18 to 30 months, is regression...

...create stable cell lines in which the expression cassette of interest is incorporated into the host...

...-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism - AAV.CAP-B22 Cell type-biased:...

...from DNA. The Genetic Code The genetic code is a set of rules for translating the information encoded ...replicated easily and efficiently in a laboratory setting. Are stable — Plasmids are stable long-term, either...Genetic Code The genetic code can be defined as a set of rules for translating the information encoded ...

...genome, while others are restricted to certain gene sets. Read More Viral Vectors Find educational guides...

... vectors that can be safely used in laboratory settings. These engineered gamma-retroviral vectors have...safety when producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus genome. Created...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain...

...Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins...

... vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have many...lentiviral vectors are also popular in clinical settings for the delivery of gene therapies. Lentiviral...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain...

...vasculature (adult marmosets), biased toward astrocytes (adult rhesus macaque) Rats, marmosets, green monkeys... including ENS (adult marmosets, infant rhesus macaques) Mice, rats, marmosets, rhesus macaques Unknown... screened in marmosets. AAV.CAP-Mac effectively transduces vasculature in adult marmosets (Chuapoco et...transduction levels in marmoset brain, with both neurons and astrocytes targeted. While marmoset liver transduction...AAV.CAP-B22 CNS: neurons and astrocytes Mice, marmosets Unknown Goertsen, Flytzanis, et al., 2022 ... of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et al, 2021 Goertsen, Flytzanis...

...side of a stop cassette, upstream of an effector gene. In cells where the stop cassette is removed during...just a fraction of a single genetically-defined subset of cells. By labeling fewer cells in a population...cells will express the effector or retain the stop cassette following recombination. Image from Isaacman-Beck... genetic tool that allows for the labeling of a subset of cells with the same genetic makeup, overcomes...effector is expressed. In cells where the stop cassette remains, the effector is not expressed and the...attP sites to drive expression of either a stop cassette or an effector gene. HDVR (2X Hepatitis Delta ... Truncating the attP site upstream of the stop cassette results in decreased efficiency of recombination...

... satellite colonies? Ampicillin Fair {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...decorative" title="Check icon" %} Just bacteria {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...Excellent No Bacteria and eukaryotes (G418) {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...purpose="decorative" title="Check icon" %} {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...Check icon" %} Carbenicillin Excellent {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...

... switch conditional knock-in cassette. As shown in Figure 2, the cassette should contain a reporter DNA...For this FLEx switch to successfully work, the cassette would need to contain the BFP coding sequence ...antisense orientation (Figure 1, top). The entire DNA cassette would be flanked by two pairs of target sites-...in the opposing orientation, you have created a cassette that will express GeneR only in the presence of...recombination event would excise the reporter from the cassette, allowing for expression of GeneR. This allows...