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TypeBlog Post... be sure to practice beforehand. Generally, two types of people will visit your poster: those that want...
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Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...gene disruption frequencies in the various cell types ranged from 18% to 65% (Maggio et al., 2014). An... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post...systems are not ideal environments for many cell types. From proteins to pathways - Ras as a model When... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...transfection, or long-term storage. But what about those pesky situations where your plasmid yield is sub-optimal... -
Experimenting with New Careers while in Grad School
TypeBlog Post...suits you best I began viewing my activities as types of experiments. I searched for new opportunities... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...perform and will save you time compared to other types of BLAST searches. If you do not know the exact... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...start thinking about languages, humans have two types of languages: one to communicate amongst each other... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post... used for plant and bacterial culture, but some types of bacteria, such as SHuffle cells, are already ... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...Journal clubs are a great way for scientists of all types to stay up to date and engaged with new advances... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...polyclonal secondary IgGs can bind to different epitopes of a primary antibody. B) Each arm of the antibody... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...alternative way to screen for loss of function phenotypes while bypassing these issues. CRISPRi libraries... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...Biology and Chemistry activities from Quatr.us Foldscopes Steve Spangler Science The Fab Lab Middle school... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...possible through other means. Easy to combine multiple types of media. High barrier to entry (people have to ... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post... of the DHS sites from 125 different human cell types from the ENCODE Project. DHS sites are the most ... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post... study (for a review click here). Alternative envelopes are powerful tools as they allow researchers to... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog Post...gene editing and allows targeting of specific cell types, it has a limited capacity to carry large DNA cargos... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post...four-base overhangs that are paired with different part types (e.g. 5’-GGAG and 3’-TACT flanking promoter parts... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...curation fields for purpose, promoters, expression types, and more, all of which help a researcher understand... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post...machinery for elongation and termination. These types of plasmids tend to be high copy number. Here, we... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...interaction varies between antibody host species and isotypes. Consequently, users need to choose the protein...