We narrowed to 588 results for: ret

1. Sweating the Small Stuff: Details in the Lab

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   ...used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu/robertsonlab...

2. Antibodies 101: Isotypes

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   ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

3. Antibodies 101: Monoclonal Antibodies

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   ...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...

4. GPCRs: How Do They Work and How Do We Study Them?

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   ...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...

5. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...more about the rules.” He said that there is no theoretical limit on the number of genes that can be manipulated...

6. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   ...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...

7. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   ...most experienced users and puts it in the hands of pretty much all investigators. Our kit makes it extremely...

8. RANbodies: Reporter Nanobody Fusions

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   ... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...

9. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   ...grad school with the plan of being a scientist. I pretty quickly figured out that I just don’t have the ...

10. Visualizing Protein Turnover In Situ

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    ...on Addgene.org Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids...

11. RaPID Detection of RNA-protein Interactions

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    ...Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction...

12. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...

13. CRISPRainbow and Genome Visualization

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    ...Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed targeting capacity. By using ...

14. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...mutants coding for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995...

15. Plasmid Tools for Microbiome Studies

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    ...with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by conjugation...

16. Human Germline Editing Using CRISPR

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    ...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...

17. Choosing the B(right)est Fluorescent Protein: Photostability

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    ...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the...

18. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular...

19. To Each HIS Own

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    ... specific proteins within a system. This can be pretty complicated given that a single cell has tens of...

20. Antibodies 101: Selecting the Right Antibody

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    ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...