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We narrowed to 949 results for: ache

Showing: 461 - 480 of 949 results

  1. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...role model Make sure the mentors you choose are teaching the stuff you want to know and display character...members speak disrespectfully of supervisors and/or each other. You are not allowed to speak to lab members... a mentor that sees you only as a paper making machine. Choose one that is a true mentor, supporting career...collaboration? If you are considering a career at a small, teaching college you might want to consider less expensive...

  2. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...it is also a good idea to run an uncut sample of each plasmid to help with troubleshooting if your digests...approximately 1:3. Since the number of base pairs for each varies, it is difficult to calculate this based ... alone. One method is to conduct 2 ligations for each plasmid you are trying to create, with varying ratios...2), or roughly equivalent numbers of colonies on each plate (unsuccessful 3).     If you have a high ...

  4. Typing CRISPR Systems

    Type
    Blog Post
    ...to arrive at this hierarchy, the main aspects of each class and type are easy to understand. Classes are...involved in plasmid competition or can hijack machinery from other CRISPR systems to carry out various...further broken down into type II, type V, and type VI, each relying on a unique Cas effector — Cas9, Cas12, ...basic diversity and the surface-level function of each type could lead you to new directions in your research...

  5. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...discuss. If you can think it, MoClo might help you achieve it! MoClo applications and specialized tools Synthetic...applications in bacteria or cell-free systems. The approach is particularly useful for producing drug precursors...a broad range of parts, including copy number machinery, chromosomal integration modules, and many more...can dream up an experiment, MoClo can help you achieve it! More recently, a CRISPR MoClo kit termed Fragmid...

  6. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ...Performing In Vivo CRISPR Screens Using the FITS Approach Caitlin Faulds, who introduced the very first...Applications and Kits We are very grateful for each and every contributor to the blog. Thank you! More...  Rachel Leeson...

  7. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    ...finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing temperatures...This edition of the contest is our traditional approach. You might decorate your lab in pipette tip snowflakes...home editions. We will select up to 3 winners for each edition and the winners will be revealed on Twitter...

  8. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...tasked the students to separate out colonies from each individual strain using the streak plate technique...University of Science and Technology, has been teaching the microbiology lab every year for over 20 years...strains are easily amenable to other experiments to teach other aspects of molecular biology. “What I'd like...

  9. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...generally publish 5-10 Hot Plasmid articles with each of our newsletters. You can find all of these articles...you haven’t got time to sit down and read through each one, plug in some headphones to learn more about...through this podcast segment. Below, we break down each of the Hot Plasmids segments into its own (highly...

  11. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...different enough in size to be easily resolved from each other. However, by choosing an enzyme or enzymes...others. When choosing restriction enzymes for this approach, it is often a good idea to choose two different...orientation so long as the expected products from each orientation are different sizes. Simply run the ...

  12. Video Library

    Type
    Protocol
    ...Addgene Careers series, we sit down with Addgene Outreach Scientist Jessica Welch to discuss her career ...scientific community. Jane Hannon Our Marketing & Outreach Specialist Jane Hannon discusses her career path...scientists looking to expand their international reach. Joel McDade Joel McDade, Addgene's Business Development...

  13. Centrifugation

    Type
    Protocol
    ...separate different components in a liquid sample. It achieves this by using centrifugal force, which is generated...keep in mind when using any centrifuge. However, each centrifuge may have specific instructions so be ... pellets form in approximately the same place in each sample. Place the centrifuge’s internal lid on the...

  14. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...Making the Right Connection by Joachim Goedhart Guest blogger Joachim Goedhart shares some strategies...molecule, are often named after vegetables; Broccoli, Spinach, and Corn, for example. Learn more about the aptamer...

  15. Editor's Choice, September 2016

    Type
    Blog Post
    ... is my pleasure to announce that we once again reached new heights on the Addgene blog: we surpassed 60,000...Proteins," was contributed by guest blogger Jessica Sacher from the University of Alberta and describes a ...currently on my way back from a very successful Outreach Trip in San Francisco and would like to give my...

  16. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ... huge, there are times when a more incremental approach is prudent. What's in the CRISPR kinome library...available from Addgene as pools provided in two sets, each with 4 gRNAs per kinase, so screens can be performed...gRNA, for use with cells that already have Cas9). Each library consists of approximately 3,000 gRNAs, as...

  18. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...sets, each of which includes plasmids with different varieties of tags, both N- and C-terminal. Each set...Central PMCID: PMC4227841. 2. Young, Carissa L., Zachary T. Britton, and Anne S. Robinson. "Recombinant ...

  19. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...genomic deletions We were inspired to produce intrachromosomal deletions based on the experiments of Kim ...to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised... RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites within a single ...

  20. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...works better for you) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled...code your Post-its and write brief descriptions on each. Place the notes sticking up out of the top of the...from webpage clippings, photographs, and file attachments. Organize your notes into notebooks, create checklists...
Showing: 461 - 480 of 949 results