We narrowed to 568 results for: bli

1. Getting to Know Expansion Microscopy

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   Blog Post

   ...experiment or evaluating ExM images in a new publication.         Why Expansion Microscopy?  Traditional...

2. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

   ...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

3. DNA Purification Without a Kit

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   Blog Post

   ...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

4. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

   ...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ...

5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   Blog Post

   ...-toxic and could be expressed in living cells, enabling the study of dynamic, physiological processes....

6. CRISPR Guide

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   Collection

   ...Experiment Web References PAM Sequences Glossary Publications CRISPR Overview Bacteria have an interesting...to check the library information and original publication to see if a library is suitable for your experiment...specified by the gRNA plus the genomic PAM sequence Publications (by topic) CRISPR basics Anders, C., Niewoehner.... D., Gross, C. A., & Rosenberg, O. S. (2019). Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

7. New Tools Enable CRISPRa for Neuroscience Applications

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   Blog Post

   ...expression of this construct is Cre-dependent, enabling spatiotemporal control over its expression. Constitutive...

8. Analyzing CRISPR Editing Results with ICE from Synthego

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   Blog Post

   ...quantitative, NGS-quality analysis of CRISPR editing - enabling a ~100-fold reduction in cost relative to NGS-...

9. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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   Blog Post

   ...removal of the residual viral particles and establishment of virus-free hiPSCs usually require about 5...

10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post

    ...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b...

11. How to Write a Scientific Review Article

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    Blog Post

    ...include every piece of information that was ever published. Obviously this isn’t possible. So, define your...

12. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post

    ...Andreas Rett, who tried to raise awareness and published his observations in a German medical journal. ...

13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post

    ...have been used successfully in experiments and published in peer reviewed journals. If you need to generate...

14. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...specific attachment sites via sequence engineering, enabling even more efficient integration activity. They...

15. Antibodies 101: Reading a Flow Plot

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    Blog Post

    ...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This...

16. Hot Plasmids - November 2023

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    Blog Post

    ...points appear as fluorescent bands in the fiber, resembling a “ticker tape” recording. The authors created...

17. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post

    ...protocol. I also recommend searching PubMed for any publications associated with the library and reading their...

18. QC Sequencing Technologies at Addgene

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    Blog Post

    ...efficiency through methods like automation. All assemblies from our pipeline are now run through an auto-align...

19. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

    ...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...

20. Hot Plasmids: Fall 2024

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    Blog Post

    ...and in ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided...