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We narrowed to 903 results for: nes

Showing: 481 - 500 of 903 results

  1. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well as a latent microglial line, treatment ...via HIV-1 promoter-driven GFP expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9...Cas9 have also been used to create mutations in genes essential to HIV-1 replication. However, this approach...modification targeting either the LTRs or essential genes. Many of these escape mutations were located near...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ... Cre-dependent transgenes can also be paired with Cre-expressing transgenic mouse lines to restrict transgene...used and emerging capsids to help you decide which ones may be suitable for your experiment. Like all viral...inducing an immune response against the capsids or transgenes. Sensory neurons and liver appear especially ...appropriate cell type-specific promoters or Cre lines. Spatial transcriptomics suggests that AAV-PHP.eB... Seed, C., & Deverman, B. E. (2019). Delivering genes across the blood-brain barrier: LY6A, a novel cellular...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    ...them. There are suspected cases of insertional mutagenesis in humans (1) and AAVs are non-enveloped viruses... estate mantra, the choice of serotype (which determines which tissues and cells an AAV can infect) can... Russell, David W. "AAV vectors, insertional mutagenesis, and cancer." Molecular Therapy 15.10 (2007):... PubMed Central PMCID: PMC3098627. 6. Salegio, Ernesto A., et al. "Axonal transport of adeno-associated...

  5. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...methods. However, most investigators use single cell clones for in vitro CRISPR experiments. The likelihood...may not be worth the cost and labor when single clones are selected. Conversely, translational research..., Yanfang, et al. "High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...mutations triggered by engineered nucleases." G3: Genes| Genomes| Genetics 5.3 (2015): 407-415. PubMed PMID...

  6. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...decision. If I’d spent my senior year isolating pheromones from various C. elegans mutants by myself, I ...etc. If you find that there is not someone (or someones) taking on these responsibilities on a team to...really starts functioning (or malfunctioning). Clear lines of communication Few people actually like meetings...purpose, an excellent leader, and perfectly clear lines of communication, but if teammates don’t trust each...

  7. Typing CRISPR Systems

    Type
    Blog Post
    ... analysis, neighborhood analysis of surrounding genes, and experimental data. In some cases, CRISPR systems...researchers began building optimal plasmids to harness the power of Class 1 systems. Class 1 CRISPR systems...cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2, which are critical for inserting...A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A. Cas9 was the first to be adapted...

  8. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ..., energy is propagated into the sample via an evanescent wave that only excites fluorophores within a ...through the fluorophore’s interaction with an evanescent energy field. Lightsheet microscopes that project...sheets of excitation light through multiple focal planes of a sample may also be useful for providing fast...Protein Resources Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...protein, you can site-specifically label it with finesse using tRNA suppression technology (Fig. 1) (Dumas...cells nonspecifically, and they cannot cross cell membranes, so they are best used either with plasma membrane... Most importantly, these compounds cross cell membranes, thus enabling FRET measurements in intact cells...Fessenden is currently an Assistant Professor in the Anesthesia Research department at Brigham and Women’s Hospital...

  11. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...sequence between two target sites. Modifying multiple genes at once. Using multiplexed gRNAs can target multiple...CRMAGE system is a fast, multiplexable method that combines CRISPR and recombineering-based MAGE (Multiplex...simultaneous multiplex editing of six different genes. This system doesn’t rely on any additional or host-dependent.... Yin, Linlin, et al. “Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs...

  12. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ... experience and judgement to a wide variety of business decisions that are not “legal” at all. Q: How ...Addgene board member? BN: Having seen so much in the business world from the mindset of a lawyer can bring a...if we do this, then what?” I hope to bring that business experience and judgement to the organization and...

  14. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...writing, clinical, regulatory, product development, business development, consulting, policy, big data and ...medicine and health, education, law, accounting, business specialties, theology, and the arts, and requiring...your work has impacted your field (i.e. finding vaccines for deadly disease, building algorithms to program...regulatory, project management, alliance management, business development, finance, and operations. To learn...

  15. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...that’s considered unlikely to change – but the illness has been fatal in 70 percent of the most recent...infection also makes it a critical component of vaccines and the target of neutralizing antibodies and ...Ebola representing the latest outbreak and earlier ones, along with links to the relevant scientific literature...

  16. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...science and business or programming were new to some, who were surprised at “how much business there is ...Last week, 20 students and four chaperones from Denis-Yarmouth Regional High School visited our labs ...

  17. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...the CRISPR/Cas9 system to precisely edit specific genes in monkeys. "Our study shows that the CRISPR/Cas9...system enables simultaneous disruption of two target genes in one step without producing off-target mutations...the news on CRISPR has gotten, consider these headlines in The New York Times and Forbes respectively:...

  18. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...to knock-out single genes in clonal populations and to knock-out thousands of genes in mixed population...targeting over 19,000 and 20,000 human and mouse genes respectively, use lentiCRISPR v2 as the backbone...

  19. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    ...My son is an engineer and my daughter has a business degree. From the very start of their undergraduate...of driving systemic change in academic career readiness training. It's been an honor to work with such... essential that we integrate inclusive career readiness training in this educational path before we lose...

  20. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ... the biomedical research community. Along these lines, Darrell recently joined the Addgene Board of Directors... various lung diseases. Chapter 10: How Darrel defines Open Science and how he practices and promotes ...open science in the lab - sharing reagents, cell lines, data, and knowledge without exclusivity. Chapter...
Showing: 481 - 500 of 903 results