We narrowed to 586 results for: ret

1. Plasmids 101: Terminators and PolyA signals

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   Blog Post

   ...Enhances Expression of Transgenes  Delivered by Retroviral Vectors. Journal of Virology. 1999. PubMed PMID...

2. The Materials Science of Optogenetics Experiments

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   Blog Post

   ...inconsistent, and behavioral results impossible to interpret. Polishing the fiber optics with diamond polishing...

3. Experimenting in an Open Source lab: from CRISPR to Cats

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   Blog Post

   ...Microsoft Research working on speech recognition, he returned to school to merge his interests in biology and...

4. Degrading DNA with Cascade-Cas3

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   Blog Post

   ...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...

5. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...not as quantitative as other methods of PCR. Theoretically scientists should be able to determine the quantity...

6. The Scientific Conference Poster Session: Tips for Success

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   Blog Post

   ...your poster on your computer screen than it is to pretend you’re standing at the session. Present with confidence...

7. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ...With a few extra lines of code, we enabled it to return guides and primers in batch, optimized for HDR....

8. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   ...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation...

9. Plasmids 101: Degron Tags

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   Blog Post

   ...addition of a small molecule, and protein levels can return to normal once the drug has been removed. The degradation...

10. Transferable Skills Guide: Managing a Team

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    Blog Post

    ... realized that at the time I could likely have retained this talented undergrad. The point here is managing...

11. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ... is docked on the membrane of the endoplasmic reticulum. And graduate students, momentarily forgetting...

12. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post

    ...of normal protein function, and ultimately misinterpretation of the protein’s cellular role. One way to...

13. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ...NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE...

14. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    ... in a protein expression strain, we suggest retransforming the plasmid into a strain meant for DNA purification...

15. Experimenting with New Careers while in Grad School

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    Blog Post

    ...a lot of attention on social media. I gained a concrete example to use to promote future attempts and ...

16. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

    ... the region between the microhomology and the retention of a single microhomology sequence. For more mechanistic...

17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post

    ...easy Very hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing Dividing ...

18. Genome-wide Screening Using CRISPR

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    Blog Post

    ... to skewed results. Selecting a cell type - Theoretically, any cell type can be used in a CRISPR screen...

19. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ...recently to explore their use in plants, including FRET tools, double dimerization GFPs, and circular permutation...

20. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...This option is not as fast as megablast, but can return longer alignments to compare with your sequencing...