We narrowed to 545 results for: SEC

1. Water Bath Protocol

   Type

   Protocol

   ... Oftentimes, the water bath will have a spot to secure the thermometer in place. Turn the water bath on...placed inside. Water bath floats can be used to secure tubes in place and water bath weights can hold ...

2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   ...be more challenging for sequences with complex secondary structure. Today, multiple companies offer long...

3. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   ...fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins to make better...

4. CRISPR 101: Off-Target Effects

   Type

   Blog Post

   ...Predicting off-target events As mentioned in the above section, there are gRNA softwares available which score...

5. Viral Vectors 101: AAV Variables That Matter

   Type

   Blog Post

   ...may your needed volume (de Backer et al., 2010).  Second, high efficiency transduction coupled with a strong...

6. Plasmids 101: Dimers and Multimers

   Type

   Blog Post

   ... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

7. Technique: Probe Phage Genomes for Host Binding Proteins

   Type

   Blog Post

   ...Therefore, in addition to their utility in the health sector, phages represent a reservoir of carbohydrate binding...

8. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   ...for which we have germline competent ESC lines. Second, the process is much quicker. It takes 3 months...

9. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   ...and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid, you could use...

10. Gibson Assembly Protocol

    Type

    Protocol

    ...annealing to the target sequence. Avoid strong secondary structures in the homology region. Hairpins in...activity of T5 Exonuclease and furthermore reduces secondary structure of ssDNA. (Rabe & Cepko, 2020). Incubate...

11. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    ...from Pyrococcus furiosus." Acta Crystallographica Section F: Structural Biology and Crystallization Communications...

12. Finding Your Science Policy Path

    Type

    Blog Post

    ...also happen at the state and local level. The big secret about science policy that I didn’t learn until ...

13. Live and Let Dye: Self-Labeling Protein Tags

    Type

    Blog Post

    ...Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer mitochondrial membrane...

14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    ...through Addgene’s viral service and the methods sections of scientific articles (gasp). To my delight, ...

15. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    ... in your system. It is very rare for a methods section to include all of the variables that need to be...

16. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    ...active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human cells. piggyBac...

17. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    ... was interested in microbes until the end of my second year! Conclusion and other pieces of advice Give...

18. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    ...PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted optical control...

19. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    ... flow cytometry. Let's briefly go through each section of this article again. In the beginning, we talked...

20. Typing CRISPR Systems

    Type

    Blog Post

    ...of CRISPR overall. Type I systems degrade large sections of DNA thanks to the dual action power of Cas3...