...effectiveness and interpretation of RNAi. Fortunately, researchers studying plants and other organisms lacking this...concatenated. hpRNA has been extensively used in plant research and many different constructs are available to...and is a popular RNAi trigger in the mammalian research community. Fortunately, many tools for shRNA in...fluorescence readout of RNAi activity. As a plant researcher, the traditional hpRNA and visual screening method...

...how and where you pursue it. It can manifest via research and data gathering, regulatory efforts, policy...fellowships have a strong focus on early career researchers who have decided to transition out of academia... host “Hill Days” where they train and bring researchers to Congress to advocate for increased science...incentivizing the real-world impacts of health services research. She is particularly interested in driving scientific...

...135. https://doi.org/10.12688/f1000research.21018.1 Munoz-Lopez M, Garcia-Perez J (2010) DNA Transposons...different transposon systems adapted for use as research tools: Sleeping beauty, PiggyBac, and Tol2.  Sleeping...encode reporter genes, mutagenic cassettes, or barcodes. When transposons are delivered to cells or a ...

... design of these constructs, allowing novice researchers to quickly and easily start genome editing (Schwartz...donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289...CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805...Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013) Heritable Custom Genomic...

...efficiency. Together, these factors prevent stem cell researchers from generating clonal knock-in lines, especially... allele. For N-terminal tagging, specifically searching for error-free knock-in at both alleles may be...for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing CRISPR/Cas9 genome-editing...Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S...

...processes that are crucial in the world of biological research, medical therapy, and even drug development (Grimm...stable, and versatile imaging tools in biological research. This does make finding the right dye for your...complicated. We hope this post helps you in your search for the perfect way to light up your science. Simon...Methods (Vol. 19, Issue 2, pp. 149–158). Nature Research. https://doi.org/10.1038/s41592-021-01338-6 Grimm...

...reagents and data, we’re also publishing research. Addgene’s research team also investigates new strategies...vector jokes and comics. Other new resources for researchers using viral vectors In addition to “going viral...

... an important animal model for research, particularly genetics research, for decades. Its short life cycle...filmed at Addgene:     If you're working in fly research, I hope you find this new technology as helpful...

...publication where you described them. Search for your article as you would search for it in PubMed and choose the... share plasmids. To achieve this goal, we will archive any plasmids you've deposited with us and distribute... your plasmids are online and available to the research community. This article was originally written...

...technology is so useful it forever enhances biological research and discovery. CRISPR is a great example (the ...infect many different types of cells. This means researchers who use VSV-G can deliver their genes of interest...autophagy, CRISPR, and so much more (find the full, searchable list here). Tips for using pMD2.G At Addgene,...

...kept Addgene at the forefront of life sciences research for the past decade. We’ve also learned along ...Summaries of the latest technologies and breakthrough research. Insights about how to navigate through all ...graduate student, post-doctoral fellow, lab manager, research technician, or Principal Investigator. How-to...

...plasmids? Do you spend a lot of time in PubMed searching for research articles? Well then, my scientist friend...Filters” page, select the category “LinkOut” and search for “Addgene”. Then check the “Link Icon” box next...

...AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service...we've made to our viral vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA...

...Human Development (NICHD) in 1986, to enable basic research by banking and distributing monoclonal antibodies... We have also created the Monoclonal Antibody Research Institute dedicated to: 1) developing new ways...Data Freedom: The Expansion of Data Sharing in Research Publications ...

...approach, originally published in Nature, is a “search-and-replace” genome editing technique that mediates...efficiency Building upon prior reverse transcriptase research, the Liu lab created and evaluated 19 PE1 variants...Liu lab contributed an improved prime editing “architecture” that can be combined with any of the PE2-PE5...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

... Mentor Learn How the Future of Research Looks to Improve the Research Process  Browse our Career Posts... Browse Wu Lab Plasmids Find Plasmids for Your Research  ...

...also noted, papers detailing Nobel Prize-winning research typically weren’t among the most cited. And, as...media too. Services like Mendeley are allowing researchers to track and share their own libraries and offering....0006022 Aragon AM. "A measure for the impact of research" (2013) Scientific Reports 3, Article number: ...

...scientists find the plasmids they need for their research, show labs how they can benefit from depositing...has been exciting to learn about all the amazing research being done with plasmids. I heard an impressive...and Harvard and the McGovern Institute for Brain Research) – and an Addgene depositing scientist – stop ...

...can help fight stress and depression, and now researchers have reported a mechanism based on studies of...exercise, show resistance to depression. For the researchers led by Dr. Jorge Ruas of the Karolinska Institutet...really wasn’t what they’d expected. "Our initial research hypothesis was that trained muscle would produce...

...know we've discussed it before, but CRISPRs allow research to move so fast." On the very same day the monkey... more precise and useful tool. A week later, researchers at the Broad Institute and MIT published another...these editing tools that are more specific to our research needs. We may even be able to alter the type of...